Recombinant Human EDIL3 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human EDIL3 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 5 x 104 cells/well are added to Recombinant Human EDIL3 coated plates (5 µg/mL, 100 µL/well), >45% will adhere after 60 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Chinese Hamster Ovary cell line, CHO-derived human EDIL3 protein
Val17-Glu480 with a C-terminal 6x His
Accession #
N-terminal Sequence
Val17, Asp24
Predicted Molecular Mass
52 kDa
60-65 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: EDIL3

EGF‑like repeat and discoidin I‑like domain‑containing protein 3 (EDIL3; also Del‑1 and integrin‑binding DEL1) is a 52 kDa extracellular matrix protein that is expressed by endothelial tissues during embryonic vascular development (1). Human EDIL3 is synthesized as a precursor with a 16 amino acid (aa) signal sequence and a 464 aa mature chain (SwissProt # O43854). The mature chain is composed of three epidermal growth factor (EGF) repeats and two discoidin‑I‑like domains (1). The second EGF repeat contains an RGD motif (1). Splicing variants produce two isoforms for human EDIL3. Isoform 2 has an A‑>G substitution at aa 66 and a deletion of
aa 67‑76 found in isoform 1. Mature human EDIL3 shares 96% aa sequence identity with mature mouse EDIL3. The RGD motif of EDIL3 binds alpha v beta 5 integrin, which, in turn, leads to increased angiogenic transcription factor HoxD3 expression (2). HoxD3activates alpha v beta 3 and uPA, resulting in the transformation of resting endothelial cells to an angiogenic state (2‑4). EDIL3 becomes quiescent at the time of birth, and is no longer expressed in normal adult tissues. It has been found, however,
re‑expressed in a number of human tumors as well as in ischemic muscles and ischemic brain tissue, which may play an important role in adult angiogenesis (2, 5‑6). EDIL3 promotes adherence and migration of endothelial cells, and acts as an endothelial cell survival agent through up‑regulation of Bcl‑2 expression (7). Exogenous application of EDIL3 has been demonstrated to augment angiogenesis and improve blood flow and tissue function in murine models of hind‑limb ischemia (6, 8), cardiac ischemia (9) and cerebral ischemia (2). EDIL3 has also been shown to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA‑1‑dependent leukocyte‑endothelial adhesion (10).

  1. Hidai, C. et al. (1998) Genes Dev. 12:21.
  2. Fan, Y. et al. (2008) Brain Res. 1219:1.
  3. Penta, K. et al. (1999) J. Biol. Chem. 274:11101.
  4. Rezaee, M. et al. (2002) Am. J. Physiol. Heart Circ. Physiol. 282:H1924.
  5. Aoka, Y. et al. (2002) Microvasc. Res. 64:148.
  6. Ho, H.K. et al. (2004) Circulation 109:1314.
  7. Pollman, M.J. et al. (1999) J. Cell Physiol. 178:359.
  8. Zhong, J. et al. (2003) J. Clin. Invest. 112:30.
  9. Kown, M.H. et al. (2003) Ann. Thorac. Surg. 76:1246.
  10. Choi, E.Y. et al. (2008) Science 322:1101.
Entrez Gene IDs
10085 (Human); 13612 (Mouse)
Alternate Names
DEL1; DEL1developmental endothelial locus-1; Developmentally-regulated endothelial cell locus 1 protein; EDIL3; EGF-like repeat and discoidin I-like domain-containing protein 3; EGF-like repeats and discoidin I-like domains 3; Integrin-binding protein DEL1; MGC26287

Citations for Recombinant Human EDIL3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Erythromycin inhibits neutrophilic inflammation and mucosal disease by upregulating DEL-1
    Authors: T Maekawa, H Tamura, H Domon, T Hiyoshi, T Isono, D Yonezawa, N Hayashi, N Takahashi, K Tabeta, T Maeda, M Oda, A Ziogas, V? Alexaki, T Chavakis, Y Terao, G Hajishenga
    JCI Insight, 2020;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA(Detection)
  2. Serum developmental endothelial locus-1 is associated with severity of sepsis in animals and humans
    Authors: WY Kim, SH Lee, DY Kim, HJ Ryu, GR Chon, YY Park, Y Fu, JW Huh, CM Lim, Y Koh, EY Choi, SB Hong
    Sci Rep, 2019;9(1):13005.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  3. Tumor derived EDIL3 modulates the expansion and osteoclastogenesis of myeloid derived suppressor cells in murine breast cancer model
    Authors: Z Kun, G Xin, W Tao, Z Chenglong, W Dongsheng, T Liang, L Tielong, X Jianru
    J Bone Oncol, 2019;16(0):100238.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. The extracellular matrix protein Edil3 stimulates osteoblast differentiation through the integrin ?5?1/ERK/Runx2 pathway
    Authors: SH Oh, JW Kim, Y Kim, MN Lee, MS Kook, EY Choi, SY Im, JT Koh
    PLoS ONE, 2017;12(11):e0188749.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay


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