Recombinant Human EDIL3 Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
Val17-Glu480 with a C-terminal 6x His
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
EGF‑like repeat and discoidin I‑like domain‑containing protein 3 (EDIL3; also Del‑1 and integrin‑binding DEL1) is a 52 kDa extracellular matrix protein that is expressed by endothelial tissues during embryonic vascular development (1). Human EDIL3 is synthesized as a precursor with a 16 amino acid (aa) signal sequence and a 464 aa mature chain (SwissProt # O43854). The mature chain is composed of three epidermal growth factor (EGF) repeats and two discoidin‑I‑like domains (1). The second EGF repeat contains an RGD motif (1). Splicing variants produce two isoforms for human EDIL3. Isoform 2 has an A‑>G substitution at aa 66 and a deletion of
aa 67‑76 found in isoform 1. Mature human EDIL3 shares 96% aa sequence identity with mature mouse EDIL3. The RGD motif of EDIL3 binds alpha v beta 5 integrin, which, in turn, leads to increased angiogenic transcription factor HoxD3 expression (2). HoxD3activates alpha v beta 3 and uPA, resulting in the transformation of resting endothelial cells to an angiogenic state (2‑4). EDIL3 becomes quiescent at the time of birth, and is no longer expressed in normal adult tissues. It has been found, however,
re‑expressed in a number of human tumors as well as in ischemic muscles and ischemic brain tissue, which may play an important role in adult angiogenesis (2, 5‑6). EDIL3 promotes adherence and migration of endothelial cells, and acts as an endothelial cell survival agent through up‑regulation of Bcl‑2 expression (7). Exogenous application of EDIL3 has been demonstrated to augment angiogenesis and improve blood flow and tissue function in murine models of hind‑limb ischemia (6, 8), cardiac ischemia (9) and cerebral ischemia (2). EDIL3 has also been shown to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA‑1‑dependent leukocyte‑endothelial adhesion (10).
- Hidai, C. et al. (1998) Genes Dev. 12:21.
- Fan, Y. et al. (2008) Brain Res. 1219:1.
- Penta, K. et al. (1999) J. Biol. Chem. 274:11101.
- Rezaee, M. et al. (2002) Am. J. Physiol. Heart Circ. Physiol. 282:H1924.
- Aoka, Y. et al. (2002) Microvasc. Res. 64:148.
- Ho, H.K. et al. (2004) Circulation 109:1314.
- Pollman, M.J. et al. (1999) J. Cell Physiol. 178:359.
- Zhong, J. et al. (2003) J. Clin. Invest. 112:30.
- Kown, M.H. et al. (2003) Ann. Thorac. Surg. 76:1246.
- Choi, E.Y. et al. (2008) Science 322:1101.
Citations for Recombinant Human EDIL3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Erythromycin inhibits neutrophilic inflammation and mucosal disease by upregulating DEL-1
Authors: T Maekawa, H Tamura, H Domon, T Hiyoshi, T Isono, D Yonezawa, N Hayashi, N Takahashi, K Tabeta, T Maeda, M Oda, A Ziogas, V? Alexaki, T Chavakis, Y Terao, G Hajishenga
JCI Insight, 2020;0(0):.
Sample Types: Cell Culture Supernates
Serum developmental endothelial locus-1 is associated with severity of sepsis in animals and humans
Authors: WY Kim, SH Lee, DY Kim, HJ Ryu, GR Chon, YY Park, Y Fu, JW Huh, CM Lim, Y Koh, EY Choi, SB Hong
Sci Rep, 2019;9(1):13005.
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Tumor derived EDIL3 modulates the expansion and osteoclastogenesis of myeloid derived suppressor cells in murine breast cancer model
Authors: Z Kun, G Xin, W Tao, Z Chenglong, W Dongsheng, T Liang, L Tielong, X Jianru
J Bone Oncol, 2019;16(0):100238.
Sample Types: In Vivo
Applications: In Vivo
The extracellular matrix protein Edil3 stimulates osteoblast differentiation through the integrin ?5?1/ERK/Runx2 pathway
Authors: SH Oh, JW Kim, Y Kim, MN Lee, MS Kook, EY Choi, SY Im, JT Koh
PLoS ONE, 2017;12(11):e0188749.
Sample Types: Whole Cells
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