Recombinant Human Ephrin-A2 Fc Chimera Protein, CF

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Recombinant Human Ephrin-A2 Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit proliferation of PC‑3 human prostate cancer cells. The ED50 for this effect is 5-25 ng/mL.
Mouse myeloma cell line, NS0-derived human Ephrin-A2 protein
Human Ephrin-A2
Accession # O43921
N-terminus C-terminus
Accession #
N-terminal Sequence
Starts at Glu27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45.1 kDa (monomer)
55-60 kDa, reducing conditions

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Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Ephrin-A2

Ephrin‑A2, also known as ELF‑1, HEK7‑L, LERK‑6, and EPLG6, is an approximately 20 kDa member of the GPI‑linked Ephrin‑A family of proteins that bind and induce the tyrosine autophosphorylation of Eph receptors. In particular, Ephrin‑A2 preferentially interacts with receptors of the EphA family of proteins. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, axon guidance and cancer progression. (1‑3). Human Ephrin‑A2 is synthesized as a 213 amino acid (aa) preproprecursor that contains a 24 aa signal peptide, a 164 aa mature chain, and a 25 aa C‑terminal propeptide that is removed prior to GPI linkage of Ephrin‑A2 to the membrane (4, 5). The mature region is structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands (1, 3), and shares 93% aa sequence identity with mouse and rat Ephrin‑A2. Ephrin‑A2 is expressed in discrete regions of the developing nervous system and limb buds (6‑9). Its distribution complements the pattern of Eph receptor expression, and this plays an important role in tissue morphogenesis (9‑11). Ephrin‑A2 exerts an axon repulsive signal which is important for the accurate pathfinding of retinal ganglion cell axons to the tectum and hippocampal axons to the lateral septum (10, 12). Its up‑regulation on astrocytes at sites of optic nerve damage may prevent re‑innervation by retinal ganglion cells (13). Ephrin‑A2 is also expressed on neural progenitor cells in the subventricular zone (SVZ). It interacts with EphA7, triggering reverse signaling through Ephrin‑A2 and inhibition of progenitor cell proliferation (10). In the developing limbs, Ephrin‑A2 regulates cartilage morphogenesis and the projection of motoneuron axons (8, 9, 14).

  1. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  2. Astin, J.W. et al. (2010) Nat. Cell Biol. 12:1194.
  3. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  4. Aasheim, H.-C. et al. (1998) Biochem. Biophys. Res. Commun. 252:378.
  5. Cerretti, D.P. and N. Nelson (1998) Genomics 47:131.
  6. Cheng, H.-J. et al. (1994) Cell 79:157.
  7. Kenmuir, C.L. et al. (2012) Anat. Rec. (Hoboken) 295:105.
  8. Ohta, K. et al. (1997) Mech. Dev. 64:127.
  9. Wada, N. et al. (2003) Dev. Biol. 264:550.
  10. Gao, P.-P. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11161.
  11. Holmberg, J. et al. (2005) Genes Dev. 19:462.
  12. Nakamoto, M. et al. (1996) Cell 86:755.
  13. Symonds, A.C.E. et al. (2007) Eur. J. Neurosci. 25:744.
  14. Eberhart, J. et al. (2000) Dev. Neurosci. 22:237.
Entrez Gene IDs
1943 (Human); 13637 (Mouse)
Alternate Names
Cek7-L; EFNA2; ELF-1; EPH-related receptor tyrosine kinase ligand 6; EphrinA2; Ephrin-A2; EPLG6HEK7 ligand; HEK7-L; HEK7-ligand; LERK6; LERK6LERK-6; ligand of eph-related kinase 6


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