Recombinant Human Ephrin-A2 Fc Chimera Protein, CF

R&D Systems | Catalog # 7856-A2

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Ephrin-A2 Fc Chimera Protein (7856-A2)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

O43921

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Ephrin-A2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Ephrin-A2 protein
Human Ephrin-A2
(Glu27-Asn188)
Accession # O43921		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Starts at Glu27

Predicted Molecular Mass

45.1 kDa (monomer)

SDS-PAGE

55-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation of PC‑3 human prostate cancer cells.
The ED50 for this effect is 5-25 ng/mL.

Formulation, Preparation, and Storage

7856-A2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-A2

Ephrin‑A2, also known as ELF‑1, HEK7‑L, LERK‑6, and EPLG6, is an approximately 20 kDa member of the GPI‑linked Ephrin‑A family of proteins that bind and induce the tyrosine autophosphorylation of Eph receptors. In particular, Ephrin‑A2 preferentially interacts with receptors of the EphA family of proteins. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, axon guidance and cancer progression. (1‑3). Human Ephrin‑A2 is synthesized as a 213 amino acid (aa) preproprecursor that contains a 24 aa signal peptide, a 164 aa mature chain, and a 25 aa C‑terminal propeptide that is removed prior to GPI linkage of Ephrin‑A2 to the membrane (4, 5). The mature region is structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands (1, 3), and shares 93% aa sequence identity with mouse and rat Ephrin‑A2. Ephrin‑A2 is expressed in discrete regions of the developing nervous system and limb buds (6‑9). Its distribution complements the pattern of Eph receptor expression, and this plays an important role in tissue morphogenesis (9‑11). Ephrin‑A2 exerts an axon repulsive signal which is important for the accurate pathfinding of retinal ganglion cell axons to the tectum and hippocampal axons to the lateral septum (10, 12). Its up‑regulation on astrocytes at sites of optic nerve damage may prevent re‑innervation by retinal ganglion cells (13). Ephrin‑A2 is also expressed on neural progenitor cells in the subventricular zone (SVZ). It interacts with EphA7, triggering reverse signaling through Ephrin‑A2 and inhibition of progenitor cell proliferation (10). In the developing limbs, Ephrin‑A2 regulates cartilage morphogenesis and the projection of motoneuron axons (8, 9, 14).

References

  1. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  2. Astin, J.W. et al. (2010) Nat. Cell Biol. 12:1194.
  3. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  4. Aasheim, H.-C. et al. (1998) Biochem. Biophys. Res. Commun. 252:378.
  5. Cerretti, D.P. and N. Nelson (1998) Genomics 47:131.
  6. Cheng, H.-J. et al. (1994) Cell 79:157.
  7. Kenmuir, C.L. et al. (2012) Anat. Rec. (Hoboken) 295:105.
  8. Ohta, K. et al. (1997) Mech. Dev. 64:127.
  9. Wada, N. et al. (2003) Dev. Biol. 264:550.
  10. Gao, P.-P. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11161.
  11. Holmberg, J. et al. (2005) Genes Dev. 19:462.
  12. Nakamoto, M. et al. (1996) Cell 86:755.
  13. Symonds, A.C.E. et al. (2007) Eur. J. Neurosci. 25:744.
  14. Eberhart, J. et al. (2000) Dev. Neurosci. 22:237.

Alternate Names

Cek7-L, EFNA2, ELF-1, EphrinA2, LERK6

Entrez Gene IDs

1943 (Human); 13637 (Mouse)

Gene Symbol

EFNA2

UniProt

O43921

Additional Ephrin-A2 Products

Product Documents for Recombinant Human Ephrin-A2 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Ephrin-A2 Fc Chimera Protein, CF

For research use only

Related Research Areas

Ephrin Ligands and Eph Receptors

Citations for Recombinant Human Ephrin-A2 Fc Chimera Protein, CF

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