Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1129-ER-050
1129-ER-01M
Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF SDS-PAGE
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Product Details
Citations (33)
FAQs
Supplemental Products
Reviews (9)

Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to block anti-ErbB2 mediated inhibition of SK‑BR‑3 human breast cancer cell proliferation. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875. The ED50 for this effect is 0.3-1.2 μg/mL in the presence of 0.6 µg/mL Anti-Human ErbB2/Her2 Monoclonal Antibody (Trastuzumab, Catalog # MAB9589).
Source
Mouse myeloma cell line, NS0-derived human ErbB2/Her2 protein
Human Erb B2
(Thr23-Thr652)
Accession # NP_004439
IEGRMD Human IgG1
(Pro100-Lys330)
6 His-tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Thr23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
96.8 kDa (monomer)
SDS-PAGE
125-130 kDa, reducing conditions
220-250 kDa, non-reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1129-ER

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and  220-250 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).

References
  1. Coussens, L. et. al. (1985) Science 230:1132.
  2. Yamamoto, T. et. al. (1986) Nature 319:230.
  3. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et. al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs
2064 (Human); 13866 (Mouse); 24337 (Rat)
Alternate Names
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Citations for Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. Cancer immunotherapy with T cells carrying bispecific receptors that mimic antibodies
    Authors: S Ahn, J Li, C Sun, K Gao, K Hirabayash, H Li, B Savoldo, R Liu, G Dotti
    Cancer Immunol Res, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T (Bioassay)
  2. d-Amino Acid Peptide Residualizing Agents for Protein Radioiodination: Effect of Aspartate for Glutamate Substitution
    Authors: M Pruszynski, CM Kang, E Koumariano, G Vaidyanath, MR Zalutsky
    Molecules, 2018;23(5):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Single molecule localization microscopy coupled with touch preparation for the quantification of trastuzumab-bound HER2
    Authors: SJ Tobin, DL Wakefield, V Jones, X Liu, D Schmolze, T Jovanovi?-
    Sci Rep, 2018;8(1):15154.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. The chimeric TAC receptor co-opts the T cell receptor yielding robust anti-tumor activity without toxicity
    Authors: CW Helsen, JA Hammill, VWC Lau, KA Mwawasi, A Afsahi, K Bezverbnay, L Newhook, DL Hayes, C Aarts, B Bojovic, GF Denisova, JM Kwiecien, I Brain, H Derocher, K Milne, BH Nelson, JL Bramson
    Nat Commun, 2018;9(1):3049.
  5. Molecular design of radiocopper-labelled Affibody molecules
    Authors: V Tolmachev, TJ Grönroos, CB Yim, J Garousi, Y Yue, S Grimm, J Rajander, A Perols, M Haaparanta, O Solin, R Ferdani, A Orlova, CJ Anderson, AE Karlström
    Sci Rep, 2018;8(1):6542.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: SPR
  6. Mabfilin and Fabfilin - New antibody-scaffold fusion formats for multispecific targeting concepts
    Authors: M Kahl, F Settele, P Knick, U Haupts, E Bosse-Doen
    Protein Expr. Purif., 2018;0(0):.
    Applications: Bioassay
  7. Efficient generation of single domain antibodies with high affinities and enhanced thermal stabilities
    Authors: N Shinozaki, R Hashimoto, K Fukui, S Uchiyama
    Sci Rep, 2017;7(1):5794.
    Species: N/A
    Sample Types: Proteins
    Applications: Bioassay
  8. Insertion of a ligand to HER2 in gB retargets HSV tropism and obviates the need for activation of the other entry glycoproteins
    Authors: B Petrovic, T Gianni, V Gatta, G Campadelli
    PLoS Pathog., 2017;13(4):e1006352.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Enhanced and long term immunogenicity of a Her-2/neu multi-epitope vaccine conjugated to the carrier CRM197 in conjunction with the adjuvant Montanide
    Authors: J Tobias, J Jasinska, K Baier, M Kundi, N Ede, C Zielinski, U Wiedermann
    BMC Cancer, 2017;17(1):118.
    Species: Mouse
    Sample Types: Serum
    Applications: Bioassay
  10. A High-Affinity Repebody for Molecular Imaging of EGFR-Expressing Malignant Tumors
    Authors: M Yun, DY Kim, JJ Lee, HS Kim, HS Kim, A Pyo, Y Ryu, TY Kim, JH Zheng, SW Yoo, H Hyun, G Oh, J Jeong, M Moon, JH Min, SY Kwon, JY Kim, E Chung, Y Hong, W Lee, HS Kim, JJ Min
    Theranostics, 2017;7(10):2620-2633.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: ELISA development
  11. Salt Effect Accelerates Site-Selective Cysteine Bioconjugation
    Authors: Peng Dai
    ACS Cent Sci, 2016;2(9):637-646.
    Applications: Bioassay
  12. A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents
    Authors: Sachdev S Sidhu
    J. Mol. Biol., 2016;0(0):.
    Applications: Western Blot Standard
  13. Pegylated Trastuzumab Fragments Acquire an Increased in Vivo Stability but Show a Largely Reduced Affinity for the Target Antigen
    Authors: F Selis, G Focà, A Sandomenic, C Marra, C Di Mauro, G Saccani Jo, S Scaramuzza, A Politano, R Sanna, M Ruvo, G Tonon
    Int J Mol Sci, 2016;17(4):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA capture
  14. A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents
    J. Mol. Biol., 2016;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Direct ELISA
  15. Neural Stem Cells Secreting Anti-HER2 Antibody Improve Survival in a Preclinical Model of HER2 Overexpressing Breast Cancer Brain Metastases.
    Authors: Kanojia D, Balyasnikova I, Morshed R, Frank R, Yu D, Zhang L, Spencer D, Kim J, Han Y, Yu D, Ahmed A, Aboody K, Lesniak M
    Stem Cells, 2015;33(10):2985-94.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  16. Exclusive Transduction of Human CD4+ T Cells upon Systemic Delivery of CD4-Targeted Lentiviral Vectors.
    Authors: Zhou Q, Uhlig K, Muth A, Kimpel J, Levy C, Munch R, Seifried J, Pfeiffer A, Trkola A, Coulibaly C, von Laer D, Wels W, Hartwig U, Verhoeyen E, Buchholz C
    J Immunol, 2015;195(5):2493-501.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. T Cells Engineered With Chimeric Antigen Receptors Targeting NKG2D Ligands Display Lethal Toxicity in Mice.
    Authors: VanSeggelen H, Hammill J, Dvorkin-Gheva A, Tantalo D, Kwiecien J, Denisova G, Rabinovich B, Wan Y, Bramson J
    Mol Ther, 2015;23(10):1600-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. A novel antibody engineering strategy for making monovalent bispecific heterodimeric IgG antibodies by electrostatic steering mechanism.
    Authors: Liu Z, Leng E, Gunasekaran K, Pentony M, Shen M, Howard M, Stoops J, Manchulenko K, Razinkov V, Liu H, Fanslow W, Hu Z, Sun N, Hasegawa H, Clark R, Foltz I, Yan W
    J Biol Chem, 2015;290(12):7535-62.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA development
  19. A HER2-specific Modified Fc Fragment (Fcab) Induces Antitumor Effects Through Degradation of HER2 and Apoptosis.
    Authors: Leung K, Batey S, Rowlands R, Isaac S, Jones P, Drewett V, Carvalho J, Gaspar M, Weller S, Medcalf M, Wydro M, Pegram R, Mudde G, Bauer A, Moulder K, Woisetschlager M, Tuna M, Haurum J, Sun H
    Mol Ther, 2015;23(11):1722-33.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  20. Selective inhibition of tumor growth by clonal NK cells expressing an ErbB2/HER2-specific chimeric antigen receptor.
    Authors: Schonfeld K, Sahm C, Zhang C, Naundorf S, Brendel C, Odendahl M, Nowakowska P, Bonig H, Kohl U, Kloess S, Kohler S, Holtgreve-Grez H, Jauch A, Schmidt M, Schubert R, Kuhlcke K, Seifried E, Klingemann H, Rieger M, Tonn T, Grez M, Wels W
    Mol Ther, 2015;23(2):330-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. The adjuvant effects of high-molecule-weight polysaccharides purified from Antrodia cinnamomea on dendritic cell function and DNA vaccines.
    Authors: Lin C, Pan I, Li Y, Pan Y, Lin M, Lu Y, Wu H, Chu C
    PLoS ONE, 2015;10(2):e0116191.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Engineering of bispecific affinity proteins with high affinity for ERBB2 and adaptable binding to albumin.
    Authors: Nilvebrant J, Astrand M, Georgieva-Kotseva M, Bjornmalm M, Lofblom J, Hober S
    PLoS ONE, 2014;9(8):e103094.
    Species: Human
    Sample Types: Protein
    Applications: Surface Plasmon Resonance
  23. Engineering multivalent antibodies to target heregulin-induced HER3 signaling in breast cancer cells.
    Authors: Kang, Jeffrey, Poovassery, Jayakuma, Bansal, Pankaj, You, Sungyong, Manjarres, Isabel M, Ober, Raimund, Ward, E Sally
    MAbs, 2014;6(2):340-53.
    Species: N/A
    Sample Types: Chip
    Applications: Bioassay
  24. Identification of anti-ErbB2 dual variable domain immunoglobulin (DVD-Ig) proteins with unique activities.
    Authors: Gu J, Yang J, Chang Q, Lu X, Wang J, Chen M, Ghayur T, Gu J
    PLoS ONE, 2014;9(5):e97292.
    Species: Human
    Sample Types:
    Applications: Bioassay
  25. Quantity and accessibility for specific targeting of receptors in tumours.
    Authors: Hussain, Sajid, Rodriguez-Fernandez, Maria, Braun, Gary B, Doyle, Francis, Ruoslahti, Erkki
    Sci Rep, 2014;4(0):5232.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. An automated immunoassay for early specificity profiling of antibodies.
    Authors: Frese, Katrin, Eisenmann, Meike, Ostendorp, Ralf, Brocks, Bodo, Pabst, Stefan
    MAbs, 2013;5(2):279-87.
    Species: Human
    Sample Types: Antibody
    Applications: Bioassay
  27. Synthesis of site-specific antibody-drug conjugates using unnatural amino acids.
    Authors: Axup, Jun Y, Bajjuri, Krishna, Ritland, Melissa, Hutchins, Benjamin, Kim, Chan Hyu, Kazane, Stephani, Halder, Rajkumar, Forsyth, Jane S, Santidrian, Antonio, Stafin, Karin, Lu, Yingchun, Tran, Hon, Seller, Aaron J, Biroc, Sandra L, Szydlik, Aga, Pinkstaff, Jason K, Tian, Feng, Sinha, Subhash, Felding-Habermann, Brunhild, Smider, Vaughn V, Schultz, Peter G
    Proc Natl Acad Sci U S A, 2012;109(40):16101-6.
    Species: N/A
    Sample Types:
    Applications: Bioassay
  28. Design of an optimized scaffold for affibody molecules.
    Authors: Feldwisch J, Tolmachev V, Lendel C, Herne N, Sjoberg A, Larsson B, Rosik D, Lindqvist E, Fant G, Hoiden-Guthenberg I, Galli J, Jonasson P, Abrahmsen L
    J. Mol. Biol., 2010;398(2):232-47.
    Applications: Bioassay
  29. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA Standard
  30. The BMP-binding protein Crossveinless 2 is a short-range, concentration-dependent, biphasic modulator of BMP signaling in Drosophila.
    Authors: Serpe M, Umulis D, Ralston A, Chen J, Olson DJ, Avanesov A, Othmer H, O'Connor MB, Blair SS
    Dev. Cell, 2008;14(6):940-53.
    Species: Drosophila
    Sample Types: Protein
    Applications: Bioassay
  31. Regression of experimental medulloblastoma following transfer of HER2-specific T cells.
    Authors: Ahmed N, Ratnayake M, Savoldo B, Perlaky L, Dotti G, Wels WS, Bhattacharjee MB, Gilbertson RJ, Shine HD, Weiss HL, Rooney CM, Heslop HE, Gottschalk S
    Cancer Res., 2007;67(12):5957-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Tandem CAR T cells targeting HER2 and IL13Ralpha2 mitigate tumor antigen escape.
    Authors: Hegde M, Mukherjee M, Grada Z, Pignata A, Landi D, Navai S, Wakefield A, Fousek K, Bielamowicz K, Chow K, Brawley V, Byrd T, Krebs S, Gottschalk S, Wels W, Baker M, Dotti G, Mamonkin M, Brenner M, Orange J, Ahmed N
    J Clin Invest, 0;126(8):3036-52.
    Species: Human
    Sample Types: Whole Cell
    Applications: CAR-T confirmation (Bioassay)
  33. Combinational targeting offsets antigen escape and enhances effector functions of adoptively transferred T cells in glioblastoma.
    Authors: Hegde M, Corder A, Chow K, Mukherjee M, Ashoori A, Kew Y, Zhang Y, Baskin D, Merchant F, Brawley V, Byrd T, Krebs S, Wu M, Liu H, Heslop H, Gottschalk S, Yvon E, Ahmed N
    Mol Ther, 0;21(11):2087-101.
    Species: Human
    Sample Types: Whole Cell
    Applications: Bioassay

FAQs

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Primary Antibodies

Human ErbB2/Her2 Antibody

WB,Flow,IHC,CyTOF-ready,InhibCellGro

Reconstitution Buffers

Reviews for Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF

Average Rating: 4.9 (Based on 9 Reviews)

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Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
By Anonymous on 02/08/2019
Application: Media additive for protein or antibody production

Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
By Anonymous on 01/30/2019
Application: Enzymatic activity in vitro

Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
By Anonymous on 12/06/2018
Application: Apoptosis assay

Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
By Anonymous on 09/18/2018
Application: Enzymatic activity in vitro

Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
By Anonymous on 01/10/2018
Application: Characterization prior to use.

Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
By Anonymous on 12/01/2017
Application: Immunoassay Standard

Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
By Anonymous on 10/20/2017
Application: CellProlif
Reason for Rating: We used HER2 protein for HER2-CAR T cells activation. It worked pretty well in the high concentration.

Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
By Anonymous on 05/18/2016
Application: Binding assay/Protein-protein interaction
Reason for Rating: signal is strong, background is low

Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
By Anonymous on 10/26/2015
Application: ELISA Control Sample Tested: Human recombinant antibody Species: Human

Used as a control for Human ErbB2/Her2 Antibody (catalog number MAB1129).