Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF: R&D Systems

Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF

(23 citations)
(4 Reviews)
  
  • Purity
    >90%, by SDS-PAGE with silver staining
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to block anti-ErbB2 mediated inhibition of SK‑BR‑3 human breast cancer cell proliferation. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875.

    The ED50 for this effect is 20-80 ng/mL in the presence of 0.6 µg/mL goat anti-hErbB2 (Catalog # AF1129).

  • Source
    Mouse myeloma cell line, NS0-derived
    Human Erb B2
    (Thr23 - Thr652)
    Accession # NP_004439
    IEGRMD Human IgG1
    (Pro100 - Lys330)
    6 His-tag
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Thr23
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    96.8 kDa (monomer)
  • SDS-PAGE

    125-130 kDa, reducing conditions

    220-250 kDa, non-reducing conditions

1129-ER
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER) blocks anti-ErbB2 mediated inhibition of the SK‑BR‑3 human breast cancer cell line. The ED50 is 20-80 ng/mL in the presence of 0.6 μg/mL Goat Anti-Human ErbB2/Her2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1129)
1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and  220-250 kDa, respectively.
Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1 - 10).

  • References:
    1. Coussens, L. et. al. (1985) Science 230:1132.
    2. Yamamoto, T. et. al. (1986) Nature 319:230.
    3. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
    4. Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
    5. Carraway, K.L. 3rd et. al.  (1994) J. Biol. Chem. 269:14303.
    6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
    7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
    8. Schlessinger, J. (2000) Cell 103:211.
    9. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
    10. Daly, R.J. (1999) Growth Factors 16:255.

  • Long Name:
    Receptor Tyrosine Protein Kinase ErbB2
  • Entrez Gene IDs:
    2064 (Human); 13866 (Mouse); 24337 (Rat)
  • Alternate Names:
    CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Insertion of a ligand to HER2 in gB retargets HSV tropism and obviates the need for activation of the other entry glycoproteins
    Authors: B Petrovic, T Gianni, V Gatta, G Campadelli
    PLoS Pathog., 2017;13(4):e1006352.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Enhanced and long term immunogenicity of a Her-2/neu multi-epitope vaccine conjugated to the carrier CRM197 in conjunction with the adjuvant Montanide
    Authors: J Tobias, J Jasinska, K Baier, M Kundi, N Ede, C Zielinski, U Wiedermann
    BMC Cancer, 2017;17(1):118.
    Species: Mouse
    Sample Type: Serum
    Application: Bioassay
  3. A High-Affinity Repebody for Molecular Imaging of EGFR-Expressing Malignant Tumors
    Authors: M Yun, DY Kim, JJ Lee, HS Kim, HS Kim, A Pyo, Y Ryu, TY Kim, JH Zheng, SW Yoo, H Hyun, G Oh, J Jeong, M Moon, JH Min, SY Kwon, JY Kim, E Chung, Y Hong, W Lee, HS Kim, JJ Min
    Theranostics, 2017;7(10):2620-2633.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: ELISA development
  4. Efficient generation of single domain antibodies with high affinities and enhanced thermal stabilities
    Authors: N Shinozaki, R Hashimoto, K Fukui, S Uchiyama
    Sci Rep, 2017;7(1):5794.
    Species: N/A
    Sample Type: Proteins
    Application: Bioassay
  5. A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents
    J. Mol. Biol., 2016;0(0):.
    Species: Human
    Sample Type: Protein
    Application: DirELISA
  6. Pegylated Trastuzumab Fragments Acquire an Increased in Vivo Stability but Show a Largely Reduced Affinity for the Target Antigen
    Authors: F Selis, G Focà, A Sandomenic, C Marra, C Di Mauro, G Saccani Jo, S Scaramuzza, A Politano, R Sanna, M Ruvo, G Tonon
    Int J Mol Sci, 2016;17(4):.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA capture
  7. T Cells Engineered With Chimeric Antigen Receptors Targeting NKG2D Ligands Display Lethal Toxicity in Mice.
    Authors: VanSeggelen H, Hammill J, Dvorkin-Gheva A, Tantalo D, Kwiecien J, Denisova G, Rabinovich B, Wan Y, Bramson J
    Mol Ther, 2015;23(10):1600-10.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. Exclusive Transduction of Human CD4+ T Cells upon Systemic Delivery of CD4-Targeted Lentiviral Vectors.
    Authors: Zhou Q, Uhlig K, Muth A, Kimpel J, Levy C, Munch R, Seifried J, Pfeiffer A, Trkola A, Coulibaly C, von Laer D, Wels W, Hartwig U, Verhoeyen E, Buchholz C
    J Immunol, 2015;195(5):2493-501.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. A HER2-specific Modified Fc Fragment (Fcab) Induces Antitumor Effects Through Degradation of HER2 and Apoptosis.
    Authors: Leung K, Batey S, Rowlands R, Isaac S, Jones P, Drewett V, Carvalho J, Gaspar M, Weller S, Medcalf M, Wydro M, Pegram R, Mudde G, Bauer A, Moulder K, Woisetschlager M, Tuna M, Haurum J, Sun H
    Mol Ther, 2015;23(11):1722-33.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Bioassay
  10. Selective inhibition of tumor growth by clonal NK cells expressing an ErbB2/HER2-specific chimeric antigen receptor.
    Authors: Schonfeld K, Sahm C, Zhang C, Naundorf S, Brendel C, Odendahl M, Nowakowska P, Bonig H, Kohl U, Kloess S, Kohler S, Holtgreve-Grez H, Jauch A, Schmidt M, Schubert R, Kuhlcke K, Seifried E, Klingemann H, Rieger M, Tonn T, Grez M, Wels W
    Mol Ther, 2015;23(2):330-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Neural Stem Cells Secreting Anti-HER2 Antibody Improve Survival in a Preclinical Model of HER2 Overexpressing Breast Cancer Brain Metastases.
    Authors: Kanojia D, Balyasnikova I, Morshed R, Frank R, Yu D, Zhang L, Spencer D, Kim J, Han Y, Yu D, Ahmed A, Aboody K, Lesniak M
    Stem Cells, 2015;33(10):2985-94.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Bioassay
  12. The adjuvant effects of high-molecule-weight polysaccharides purified from Antrodia cinnamomea on dendritic cell function and DNA vaccines.
    Authors: Lin C, Pan I, Li Y, Pan Y, Lin M, Lu Y, Wu H, Chu C
    PLoS ONE, 2015;10(2):e0116191.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. A novel antibody engineering strategy for making monovalent bispecific heterodimeric IgG antibodies by electrostatic steering mechanism.
    Authors: Liu Z, Leng E, Gunasekaran K, Pentony M, Shen M, Howard M, Stoops J, Manchulenko K, Razinkov V, Liu H, Fanslow W, Hu Z, Sun N, Hasegawa H, Clark R, Foltz I, Yan W
    J Biol Chem, 2015;290(12):7535-62.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA development
  14. Engineering multivalent antibodies to target heregulin-induced HER3 signaling in breast cancer cells.
    Authors: Kang, Jeffrey, Poovassery, Jayakuma, Bansal, Pankaj, You, Sungyong, Manjarres, Isabel M, Ober, Raimund, Ward, E Sally
    MAbs, 2014;6(2):340-53.
    Species: N/A
    Sample Type: Chip
    Application: Bioassay
  15. Engineering of bispecific affinity proteins with high affinity for ERBB2 and adaptable binding to albumin.
    Authors: Nilvebrant J, Astrand M, Georgieva-Kotseva M, Bjornmalm M, Lofblom J, Hober S
    PLoS ONE, 2014;9(8):e103094.
    Species: Human
    Sample Type: Protein
    Application: Surface Plasmon Resonance
  16. Quantity and accessibility for specific targeting of receptors in tumours.
    Authors: Hussain, Sajid, Rodriguez-Fernandez, Maria, Braun, Gary B, Doyle, Francis, Ruoslahti, Erkki
    Sci Rep, 2014;4(0):5232.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Identification of anti-ErbB2 dual variable domain immunoglobulin (DVD-Ig) proteins with unique activities.
    Authors: Gu J, Yang J, Chang Q, Lu X, Wang J, Chen M, Ghayur T, Gu J
    PLoS ONE, 2014;9(5):e97292.
    Species: Human
    Sample Type:
    Application: Bioassay
  18. An automated immunoassay for early specificity profiling of antibodies.
    Authors: Frese, Katrin, Eisenmann, Meike, Ostendorp, Ralf, Brocks, Bodo, Pabst, Stefan
    MAbs, 2013;5(2):279-87.
    Species: Human
    Sample Type: Antibody
    Application: Bioassay
  19. Synthesis of site-specific antibody-drug conjugates using unnatural amino acids.
    Authors: Axup, Jun Y, Bajjuri, Krishna, Ritland, Melissa, Hutchins, Benjamin, Kim, Chan Hyu, Kazane, Stephani, Halder, Rajkumar, Forsyth, Jane S, Santidrian, Antonio, Stafin, Karin, Lu, Yingchun, Tran, Hon, Seller, Aaron J, Biroc, Sandra L, Szydlik, Aga, Pinkstaff, Jason K, Tian, Feng, Sinha, Subhash, Felding-Habermann, Brunhild, Smider, Vaughn V, Schultz, Peter G
    Proc Natl Acad Sci U S A, 2012;109(40):16101-6.
    Species: N/A
    Sample Type:
    Application: Bioassay
  20. Design of an optimized scaffold for affibody molecules.
    Authors: Feldwisch J, Tolmachev V, Lendel C, Herne N, Sjoberg A, Larsson B, Rosik D, Lindqvist E, Fant G, Hoiden-Guthenberg I, Galli J, Jonasson P, Abrahmsen L
    J. Mol. Biol., 2010;398(2):232-47.
    Application: Bioassay
  21. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  22. The BMP-binding protein Crossveinless 2 is a short-range, concentration-dependent, biphasic modulator of BMP signaling in Drosophila.
    Authors: Serpe M, Umulis D, Ralston A, Chen J, Olson DJ, Avanesov A, Othmer H, O'Connor MB, Blair SS
    Dev. Cell, 2008;14(6):940-53.
    Species: Drosophila
    Sample Type: Protein
    Application: Bioassay
  23. Regression of experimental medulloblastoma following transfer of HER2-specific T cells.
    Authors: Ahmed N, Ratnayake M, Savoldo B, Perlaky L, Dotti G, Wels WS, Bhattacharjee MB, Gilbertson RJ, Shine HD, Weiss HL, Rooney CM, Heslop HE, Gottschalk S
    Cancer Res., 2007;67(12):5957-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 23 Citations
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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Human ErbB2/Her2 Antibody

WB, Flow, IHC, CyTOF-ready, InhibCellGro AF1129 3  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Recombinant Proteins
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Recombinant Human IgG1 Fc, CF

BA 110-HG 66
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Average Rating: 5 (Based on 4 reviews)

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We have 1 review tested in 1 species: Human.
We have 4 reviews tested in 4 applications: Immunoassay Standard, Cell proliferation, Binding assay/Protein-protein interaction, ELISA Control.

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(1)
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(1)
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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Immunoassay Standard Anonymous 12/01/2017
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Summary

ApplicationImmunoassay Standard
  Excellent
 Cell proliferation Anonymous 10/20/2017
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Summary

ApplicationCell proliferation
Reason for RatingWe used HER2 protein for HER2-CAR T cells activation. It worked pretty well in the high concentration.
  Excellent
 Binding assay/Protein-protein interaction Anonymous 05/18/2016
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Summary

ApplicationBinding assay/Protein-protein interaction
Reason for Ratingsignal is strong, background is low
  Excellent
 ELISA Control Human Anonymous 10/26/2015
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Summary

ApplicationELISA Control
Sample TestedHuman recombinant antibody
SpeciesHuman

Other Experimental Details

Other Experimental DetailsUsed as a control for Human ErbB2/Her2 Antibody (catalog number MAB1129).

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