Recombinant Human F13A1 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10179-F1-050
F13A1 Protein Enzyme Activity
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Product Details
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Recombinant Human F13A1 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to release DNP from Abz-NE(CAD-DNP)EQVSPLTLLK-OH. The specific activity is >13.0 pmol/min/μg, as measured under the described conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human F13A1 protein
Ser2-Met732
with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.
Predicted Molecular Mass
84 kDa
SDS-PAGE
76-88 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10179-F1

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, Glycerol and TCEP.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

Materials
  • Assay Buffer: 50 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% Brij-35, pH 7.5 (TCNB)
  • Recombinant Human F13A1 (rhF13A1) (Catalog # 10179-F1)
  • Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE)
  • Substrate: Abz-NE(CAD-DNP)EQVSPLTLLK-OH (Zedira, Catalog # A101), 5 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Florescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Activate rhF13A1 at 100 µg/mL with 40 µg/mL rhThrombin in Assay Buffer for 30 minutes at 37 °C.
  2. Dilute activated rhF13A1 to 20 µg/mL in Assay Buffer.
  3. Dilute Substrate to 100 µM in Assay Buffer.
  4. Load in plate 50 µL of 20 µg/mL activated rhF13-A1, and start the reaction by adding 50 µL of 100 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 100 µM Substrate.
  5. Read at excitation and emission wavelengths at 313 nm and 418 nm, respectively, in kinetic mode for 5 minutes.
  6. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

 *Adjusted for Substrate Blank

**Derived using calibration standard Abz-Gly-OH (Bachem, Catalog # E-2920)

Per Well:
  • rhF13A1: 1 µg
  • Substrate: 50 µM

Data Images

Enzyme Activity Enzyme Activity View Larger

Recombinant Human F13A1 His-tag (Catalog # 10179-F1) is measured byits ability to release DNP from Abz-NE(CAD-DNP)EQVSPLTLLK-OH. The activity(orange) is approximately 2-fold higher than the competitor's F13A1 (green).

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human F13A1 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing a band at ~82 kDa under reducing conditions.

Reconstitution Calculator

Reconstitution Calculator

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Background: F13A1

Coagulation Factor XIIIa1 (F13a1) is a member of the transglutaminase family which includes F13A1 and TGM1-7 (1). F13 in the plasma is a tetrameric complex composed of two alpha (A) and two beta (B) chains where the A subunit is a transglutaminase zymogen and the B subunit is an inhibitory glycoprotein with no enzymatic function (2).  Activation by thrombin and calcium ions results in the formation of the catalytically active transglutaminase F13a composed of an alpha chain homodimer capable of forming gamma-glutamyl-epsilon-lysine cross-links. The 83 kDa F13a monomer has an N-terminal activation peptide and a beta sandwich preceding the catalytic core with catalytic triad active site and two C-terminal beta barrels (3). The active homodimer is intracellular in platelets, megakaryocytes, monocytes and macrophages. The primary physiological outcome of the catalytic activity of F13a is cross-linking of fibrin and anti-plasmin to stabilize the fibrin clot (4,5). However, in addition to cross-linking fibrin, F13a is capable of cross-linking many substrates involved in complement activation, coagulation, inflammatory and immune responses and extracellular matrix organization (6). Cross-linking of key substrates by F13a has been directly shown to play a role in in atherosclerosis (7), wound healing (8), angiogenesis (9,10), maintaining pregnancy (11), ECM deposition, osteoblast differentiation and bone remodeling (12), and immune defense (13). F13A has also been detected as a marker in acute promyelocytic leukemia (APL)(14) and expression is considered of value for diagnosis and prognosis for leukemia-associated immunophenotype. Congenital deficiency results in bleeding manifestations including intercranial hemorrhage (15), poor wound healing (17), and spontaneous abortions (17) that can be treated with F13 (18).

References
  1. Griffin, M. et al. (2002) Biochem. J. 368:377.
  2. Muszbek, L. et al. (1999) Thromb. Res. 94:271.
  3. Yee, V. C. et al. (1994) Proc. Natl. Acad. Sci. USA 91:7296.
  4. Lord, S. T. et al. (2011) Aterioscler. Thromb. Vasc. Biol. 31:494.
  5. Fraser, S. R. et al. (2011) Blood 117:6371.
  6. Nikolajsen, C. L. et al. (2014) J. Biol. Chem. 289:6526.
  7. AbdAlla, S. et al. (2004) Cell. 119:343.
  8. Nahrendorf, M. et al. (2006) Circulation 113:1196.
  9. Dardik, R. et al. (2006) Thromb. Haemost. 95:546.
  10. Dardik, R. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:526
  11. Asahina, T. et al. (2000) Placenta. 21:388.
  12. Piercy-Kotb, S. A. et al. (2012) J. Cell Physiol. 227:2936.
  13. Richardson, V. R. et al. (2012) Br. J. Haematol. 160:116.
  14. Simon, A. et al. (2012) Cytometry B. Clin. Cytom. 82:209.
  15. Naderi, M. et al. (2015) Hematology. 20:112.
  16. Inbal, A. et al. (2005) Thromb. Haemost. 94:432.
  17. Inbal, A. and L. Muszbek. (2003) Semin. Thromb. Hemost. 29:171
  18. Naderi, M. et al. (2016) Iran J. Pharm. Res. 15:635.
Long Name
Coagulation Factor XIII, A1 Polypeptide
Entrez Gene IDs
2162 (Human)
Alternate Names
bA525O21.1 (coagulation factor XIII, A1 polypeptide); coagulation factor XIII A chain; coagulation factor XIII, A1 polypeptide; Coagulation factor XIIIa; EC 2.3.2.13; F13A; F13A1; F13Acoagulation factor XIII, A polypeptide; factor XIIIa; fibrin stabilizing factor, A subunit; fibrinoligase; FSF, A Subunit; Protein-glutamine gamma-glutamyltransferase A chain; TGase; Transglutaminase A chain; transglutaminase. plasma

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