Recombinant Human FGF-18 Protein, CF

    
Datasheet / CoA / SDS
Product Details
FAQs
Supplemental Products
Reviews
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 for this effect is 4-24 ng/mL
  • Source
    E. coli-derived human FGF-18 protein
    Glu28-Ala207, with a N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    21 kDa
  • SDS-PAGE
    19-24 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

8988-F18
 
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and Trehalose.
 
Reconstitution Reconstitute at 500 μg/mL in water.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
Recombinant Human FGF-18 (Catalog # 8988-F18) induces cell proliferation of the Baf3mouse pro-B cells transfected with human FGF RIIIc. The ED50for this effect is 4-24 ng/mL.
Background: FGF-18
Fibroblast Growth Factor 18 (FGF-18) is a 20 kDa protein that plays an important role in skeletal development and bone homeostasis (1). Mature human FGF-18 shares 99% amino acid sequence identity with mouse and rat FGF-18 (2). It is expressed in embryonic somites and the neural fold (3), adult lung (2), cerebellar and hippocampal neurons (4), hair follicle root sheath cells (5), and osteogenic mesenchymal cells (6). FGF-18 binds to FGF R2c, FGF R3c (4, 7) as well as the Golgi protein GLG1 (8) and induces the proliferation of astrocytes and microglia, vascular endothelial cells, dermal fibroblasts, papilla cells, and keratinocytes (4, 5, 9). FGF-18 is required for normal skeletal development (6). It recruits osteoclasts and osteoblasts to the growth plate, promotes osteoclast formation and function, inhibits osteoblast differentiation, promotes skeletal vascularization, and induces chondrocyte hypertrophy and cartilage matrix formation (6, 7, 10, 11).
  • References:
    1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
    2. Ohbayashi, N. et al. (1998) J. Biol. Chem. 273:18161.
    3. Ohuchi, H. et al. (2000) Mech. Dev. 95:55.
    4. Hoshikawa, M. et al. (2002) Brain Res. Mol. Brain Res. 105:60.
    5. Kawano, M. et al. (2005) J. Invest. Dermatol. 124:877.
    6. Ohbayashi, N. et al. (2002) Genes Dev. 16:870.
    7. Davidson, D. et al. (2005) J. Biol. Chem. 280:20509.
    8. Miyaoka, Y. et al. (2010) Development 137:159.
    9. Hu, M.C. et al. (1998) Mol. Cell. Biol. 18:6063.
    10. Liu, Z. et al. (2007) Dev. Biol. 302:80.
    11. Shimoaka, T. et al. (2002) J. Biol. Chem. 277:7493.
  • Long Name:
    Fibroblast Growth Factor 18
  • Entrez Gene IDs:
    8817 (Human)
  • Alternate Names:
    FGF18; FGF-18; FGF-18ZFGF5; fibroblast growth factor 18; zFGF5
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 7 (Deionized Water)

RB07
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human FGF-18 Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Related Products
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee