Recombinant Human FGF-18 Protein, CF Summary
Glu28-Ala207, with a N-terminal Met
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in water.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Human FGF-18 (Catalog # 8988-F18) induces cell proliferation of the Baf3 mouse pro-B cells transfected with human FGF RIIIc. The ED50 for this effect is 4-24 ng/mL.
Fibroblast Growth Factor 18 (FGF-18) is a 20 kDa protein that plays an important role in skeletal development and bone homeostasis (1). Mature human FGF-18 shares 99% amino acid sequence identity with mouse and rat FGF-18 (2). It is expressed in embryonic somites and the neural fold (3), adult lung (2), cerebellar and hippocampal neurons (4), hair follicle root sheath cells (5), and osteogenic mesenchymal cells (6). FGF-18 binds to FGF R2c, FGF R3c (4, 7) as well as the Golgi protein GLG1 (8) and induces the proliferation of astrocytes and microglia, vascular endothelial cells, dermal fibroblasts, papilla cells, and keratinocytes (4, 5, 9). FGF-18 is required for normal skeletal development (6). It recruits osteoclasts and osteoblasts to the growth plate, promotes osteoclast formation and function, inhibits osteoblast differentiation, promotes skeletal vascularization, and induces chondrocyte hypertrophy and cartilage matrix formation (6, 7, 10, 11).
- Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
- Ohbayashi, N. et al. (1998) J. Biol. Chem. 273:18161.
- Ohuchi, H. et al. (2000) Mech. Dev. 95:55.
- Hoshikawa, M. et al. (2002) Brain Res. Mol. Brain Res. 105:60.
- Kawano, M. et al. (2005) J. Invest. Dermatol. 124:877.
- Ohbayashi, N. et al. (2002) Genes Dev. 16:870.
- Davidson, D. et al. (2005) J. Biol. Chem. 280:20509.
- Miyaoka, Y. et al. (2010) Development 137:159.
- Hu, M.C. et al. (1998) Mol. Cell. Biol. 18:6063.
- Liu, Z. et al. (2007) Dev. Biol. 302:80.
- Shimoaka, T. et al. (2002) J. Biol. Chem. 277:7493.
Citations for Recombinant Human FGF-18 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Amniotic suspension allograft improves pain and function in a rat meniscal tear-induced osteoarthritis model
Authors: KA Kimmerling, AH Gomoll, J Farr, KC Mowry
Arthritis Research & Therapy, 2022;24(1):63.
Sample Types: In Vivo
Applications: In Vivo
Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells
Authors: K Murata, U Jadhav, S Madha, J van Es, J Dean, A Cavazza, K Wucherpfen, F Michor, H Clevers, RA Shivdasani
Cell Stem Cell, 2020;0(0):.
Sample Types: Whole Cells
Applications: Cell Culture
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