Recombinant Human FGF-18 Protein, CF

Catalog # Availability Size / Price Qty
8988-F18-050
Recombinant Human FGF-18 Protein Bioactivity
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Citations (2)
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Recombinant Human FGF-18 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 for this effect is 4-24 ng/mL
Source
E. coli-derived human FGF-18 protein
Glu28-Ala207, with a N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
21 kDa
SDS-PAGE
19-24 kDa, reducing conditions

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8988-F18

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8988-F18

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human FGF-18 Protein Bioactivity View Larger

Recombinant Human FGF-18 (Catalog # 8988-F18) induces cell proliferation of the Baf3 mouse pro-B cells transfected with human FGF RIIIc. The ED50 for this effect is 4-24 ng/mL.

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Background: FGF-18

Fibroblast Growth Factor 18 (FGF-18) is a 20 kDa protein that plays an important role in skeletal development and bone homeostasis (1). Mature human FGF-18 shares 99% amino acid sequence identity with mouse and rat FGF-18 (2). It is expressed in embryonic somites and the neural fold (3), adult lung (2), cerebellar and hippocampal neurons (4), hair follicle root sheath cells (5), and osteogenic mesenchymal cells (6). FGF-18 binds to FGF R2c, FGF R3c (4, 7) as well as the Golgi protein GLG1 (8) and induces the proliferation of astrocytes and microglia, vascular endothelial cells, dermal fibroblasts, papilla cells, and keratinocytes (4, 5, 9). FGF-18 is required for normal skeletal development (6). It recruits osteoclasts and osteoblasts to the growth plate, promotes osteoclast formation and function, inhibits osteoblast differentiation, promotes skeletal vascularization, and induces chondrocyte hypertrophy and cartilage matrix formation (6, 7, 10, 11).

References
  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Ohbayashi, N. et al. (1998) J. Biol. Chem. 273:18161.
  3. Ohuchi, H. et al. (2000) Mech. Dev. 95:55.
  4. Hoshikawa, M. et al. (2002) Brain Res. Mol. Brain Res. 105:60.
  5. Kawano, M. et al. (2005) J. Invest. Dermatol. 124:877.
  6. Ohbayashi, N. et al. (2002) Genes Dev. 16:870.
  7. Davidson, D. et al. (2005) J. Biol. Chem. 280:20509.
  8. Miyaoka, Y. et al. (2010) Development 137:159.
  9. Hu, M.C. et al. (1998) Mol. Cell. Biol. 18:6063.
  10. Liu, Z. et al. (2007) Dev. Biol. 302:80.
  11. Shimoaka, T. et al. (2002) J. Biol. Chem. 277:7493.
Long Name
Fibroblast Growth Factor 18
Entrez Gene IDs
8817 (Human)
Alternate Names
FGF18; FGF-18; FGF-18ZFGF5; fibroblast growth factor 18; zFGF5

Citations for Recombinant Human FGF-18 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Amniotic suspension allograft improves pain and function in a rat meniscal tear-induced osteoarthritis model
    Authors: KA Kimmerling, AH Gomoll, J Farr, KC Mowry
    Arthritis Research & Therapy, 2022;24(1):63.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  2. Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells
    Authors: K Murata, U Jadhav, S Madha, J van Es, J Dean, A Cavazza, K Wucherpfen, F Michor, H Clevers, RA Shivdasani
    Cell Stem Cell, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture

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