Recombinant Human FGF-18 Protein, CF

  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 for this effect is typically 4-24 ng/mL
  • Source
    E. coli-derived Glu28-Ala207, with a N-terminal Met
  • Accession #
  • N-terminal Sequence
  • Predicted Molecular Mass
    21 kDa
    19-24 kDa, reducing conditions
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human FGF-18 (Catalog # 8988-F18) induces cell proliferation of the Baf3mouse pro-B cells transfected with human FGF RIIIc. The ED50for this effect is typically
4-24 ng/mL.
Background: FGF-18
Fibroblast Growth Factor 18 (FGF-18) is a 20 kDa protein that plays an important role in skeletal development and bone homeostasis (1). Mature human FGF-18 shares 99% amino acid sequence identity with mouse and rat FGF-18 (2). It is expressed in embryonic somites and the neural fold (3), adult lung (2), cerebellar and hippocampal neurons (4), hair follicle root sheath cells (5), and osteogenic mesenchymal cells (6). FGF-18 binds to FGF R2c, FGF R3c (4, 7) as well as the Golgi protein GLG1 (8) and induces the proliferation of astrocytes and microglia, vascular endothelial cells, dermal fibroblasts, papilla cells, and keratinocytes (4, 5, 9). FGF-18 is required for normal skeletal development (6). It recruits osteoclasts and osteoblasts to the growth plate, promotes osteoclast formation and function, inhibits osteoblast differentiation, promotes skeletal vascularization, and induces chondrocyte hypertrophy and cartilage matrix formation (6, 7, 10, 11).
  • References:
    1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
    2. Ohbayashi, N. et al. (1998) J. Biol. Chem. 273:18161.
    3. Ohuchi, H. et al. (2000) Mech. Dev. 95:55.
    4. Hoshikawa, M. et al. (2002) Brain Res. Mol. Brain Res. 105:60.
    5. Kawano, M. et al. (2005) J. Invest. Dermatol. 124:877.
    6. Ohbayashi, N. et al. (2002) Genes Dev. 16:870.
    7. Davidson, D. et al. (2005) J. Biol. Chem. 280:20509.
    8. Miyaoka, Y. et al. (2010) Development 137:159.
    9. Hu, M.C. et al. (1998) Mol. Cell. Biol. 18:6063.
    10. Liu, Z. et al. (2007) Dev. Biol. 302:80.
    11. Shimoaka, T. et al. (2002) J. Biol. Chem. 277:7493.
  • Long Name:
    Fibroblast Growth Factor 18
  • Entrez Gene IDs:
    8817 (Human)
  • Alternate Names:
    FGF18; FGF-18ZFGF5; fibroblast growth factor 18; zFGF5
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