Recombinant Human Fibulin 3 Protein, CF

Catalog # Availability Size / Price Qty
8416-FB-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Fibulin 3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.4-2 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Fibulin 3 protein
Gln18-Phe493 with N-terminal HA tag
Accession #
N-terminal Sequence
Analysis
Tyr (of HA tag)
Predicted Molecular Mass
54 kDa
SDS-PAGE
52-71 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8416-FB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Fibulin 3

Human Fibulin-3, also called EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP-1), is an approximately 57 kDa member of the fibulin family of proteins (1). It was originally identified in fibroblasts from a Werner syndrome patient with premature aging (2). Along with Fibulin-4 and -5, Fibulin-3 is referred to as a class II, or short fibulin (3). Fibulin-3 contains five calcium-binding EGF-like motifs and a fibulin-type C terminus (1). Human Fibulin-3 shares 95% and 93% amino acid sequence identity with mouse and rat Fibulin-3, respectively. It has been shown to be susceptible to MMP-mediated proteolysis (4). Fibulin-3 appears to be involved in the proper function of elastic tissues. Knockout mice are viable, but display disrupted elastic fibers in fascia connective tissue of the body wall (5, 6). In humans, Fibulin-3 mutations have been associated with Doyne honeycomb retinal dystrophy (7). Its expression is up-regulated in late, metastatic stages of carcinomas, suggesting that it may play a role in cancer (8, 9). Fibulin-3 promotes the growth of gliomas and may have a pro-angiogenic role in this cancer type (10, 11). In contrast, Fibulin-3 has been shown to negatively modulate the invasiveness of lung cancer cells and inhibit self-renewal of lung cancer stem cells (12-14).

References
  1. Giltay, R. et al. (1999) Matrix Biol. 18:469.
  2. Lecka-Czernik, B. et al. (1995) Mol. Cell. Biol. 15:120.
  3. Yanagisawa, H. and E.C. Davis (2010) Int. J. Biochem. Cell Biol. 42:1084.
  4. Djokic, J. et al. (2013) J. Biol. Chem. 288:22821.
  5. Kobayashi, N. et al. (2007) J. Biol. Chem. 282:11805.
  6. McLaughlin, P.J. et al. (2007) Hum. Mol. Genet. 16:3059.
  7. Stone, E.M. et al. (1999) Nat. Genet. 22:199.
  8. Hwang, C.F. et al. (2010) J. Pathol. 222:367.
  9. En-lin, S. et al. (2010) Gynecol. Oncol. 117:417.
  10. Hu, B. et al. (2012) Cancer Res. 72:3873.
  11. Nandhu, M.S. et al. (2014) Cancer Res. [Epub ahead of print].
  12. Xu, S. et al. (2014) Oncol. Rep. 31:79.
  13. Chen, X. et al. (2014) Carcinogenesis 35:1707.
  14. Kim, I.G. et al. (2014) Oncogene 33:3908.
Entrez Gene IDs
2202 (Human); 216616 (Mouse); 305604 (Rat)
Alternate Names
DHRD; EFEMP1; EGF containing fibulin-like extracellular matrix protein 1; EGF-containing fibulin-like extracellular matrix protein 1; Extracellular protein S1-5; FBLN3; FBLN3DRAD; FBNLFLJ35535; FIBL-3; Fibrillin-like protein; fibrillin-like; Fibulin 3; fibulin-3; MGC111353; MLVT; MTLV; S1-5

Citation for Recombinant Human Fibulin 3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Fibulin-3 Attenuates Phosphate-Induced Vascular Smooth Muscle Cell Calcification by Inhibition of Oxidative Stress
    Authors: TTD Luong, N Schelski, B Boehme, M Makridakis, A Vlahou, F Lang, B Pieske, I Alesutan, J Voelkl
    Cell. Physiol. Biochem., 2018;46(4):1305-1316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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