Recombinant Human Frizzled-9 Fc Chimera Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Frizzled-9 Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an ED50 of 15-90 ng/mL.
Human embryonic kidney cell, HEK293-derived human Frizzled-9 protein
Human Frizzled-9
Accession # O00144
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
44 kDa
54-63 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Frizzled-9

Frizzled-9 (Fzd9, previously called Fzd3) belongs to the Frizzled family of seven-transmembrane glycoprotein receptors, which are signaling receptors for the family of secreted Wnt ligands (1-3). Mature human Frizzled-9 consists of a 207 amino acid (aa) N-terminal extracellular domain (ECD), the 7TM region, and a 62 aa C-terminal cytoplasmic domain with a PDZ binding motif. The ECD includes a cysteine-rich region that binds Wnts and is highly conserved among Frizzleds. Within aa 23-185 of the N-terminal ECD, human Frizzled-9 shares 97% aa identity with mouse and rat Frizzled-9. The human Frizzled-9 gene maps to a large region that is deleted in Williams-Beuren syndrome, a developmental disorder affecting personality and cognition (2). Frizzled-9 mRNA is expressed in the central nervous system and myotomes during development, and it persists in the hippocampus throughout life (2-4). Adults also show expression in heart, testis, and skeletal muscle (2-4). Cellular expression is detected in proliferating neural precursors, B lymphocyte progenitors, differentiating osteoblasts, and developing myotubes of skeletal muscle (4-7). Mice lacking Frizzled-9 exhibit defects in learning, B lymphocyte development, and bone mineralization (4-6, 8). Frizzled-9 can signal through both canonical and non-canonical pathways (6, 9). Its binding to Wnt-7a promotes the activation of PPAR gamma, expression of Cadherin and Sprouty, and reversal of non-small cell lung cancer cell transformation (10-12).

  1. Dijksterhuis, J.P. et al. (2014) Br. J. Pharmacol. 171:1195.
  2. Wang, Y.K. et al. (1997) Hum. Mol. Genet. 6:465.
  3. Wang, Y.K. et al. (1999) Genomics 57:235.
  4. Zhao, C. et al. (2005) Development 132:2917.
  5. Ranheim, E.A. et al. (2005) Blood 105:2487.
  6. Albers, J. et al. (2011) J. Cell Biol. 192:1057.
  7. Aviles, E.C. et al. (2014) Front. Cell. Neurosci. 8:110.
  8. Heilmann, A. et al. (2013) PLoS One 8:e84232.
  9. Karasawa, T. et al. (2002) J. Biol. Chem. 277:37479.
  10. Winn, R.A. et al. (2005) J. Biol. Chem. 280:19625.
  11. Winn, R.A. et al. (2006) J. Biol. Chem. 281:26943.
  12. Tennis, M.A. et al. (2010) Mol. Cancer Res. 8:833.
Entrez Gene IDs
8326 (Human); 14371 (Mouse)
Alternate Names
CD349 antigen; CD349; frizzled (Drosophila) homolog 9; frizzled homolog 9 (Drosophila); Frizzled9; Frizzled-9; fz-9; FZD3fzE6; FZD9; FzE6; hFz9

Citation for Recombinant Human Frizzled-9 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy
    Authors: R Chidiac, M Abedin, G Macleod, A Yang, PE Thibeault, LL Blazer, JJ Adams, L Zhang, H Roehrich, HN Jo, S Seshagiri, SS Sidhu, HJ Junge, S Angers
    Embo Molecular Medicine, 2021;0(0):e13977.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay


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