Recombinant Human GDNF Protein, Animal-Free

R&D Systems | Catalog # BT-GDNF-AFL

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Key Product Details

Learn more about Animal-Free Recombinant Proteins

Source

E. coli

Accession Number

NP_000505.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all GDNF Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human GDNF protein
Gly113-Ile211
Produced using non-animal reagents in an animal-free laboratory.

Purity

>97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly113 & Met

Predicted Molecular Mass

16 kDa

SDS-PAGE

8 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells.
The ED50 for this effect is 0.750-9.00 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR). The specific activity of recombinant human GDNF is >5.0 x 105 units/mg, which is calibrated against the human GDNF Reference Standard (NIBSC code: 09/266).

Scientific Data Images for Recombinant Human GDNF Protein, Animal-Free

Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human GDNF.

Equivalent bioactivity of GMP (BT-GDNF-GMP), Animal-Free (Catalog # BT-GDNF-AFL) and RUO (BT-GDNF) grades of Recombinant Human GDNF as measured in a cell proliferationassay using the cell proliferation of SH-SY5Y cells (orange, green, red, respectively).

Animal-Free™ Recombinant Human GDNF Protein Bioactivity.

Animal-Free™ Recombinant Human GDNF Protein (Catalog # BT-GDNF-AFL) stimulates proliferation in the SH-SY5Y human neuroblastoma cell line. The ED50 for this effect is 0.750-9.00 ng/mL in the presence of Recombinant Human GFRa-1/GDNF Ra-1 Fc Chimera (714-GR).

Formulation, Preparation, and Storage

BT-GDNF-AFL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Long Name

Glial Cell line-derived Growth Factor

Alternate Names

ATF, HFB1-GDNF, HGDNF, HSCR3

Entrez Gene IDs

2668 (Human); 25453 (Rat)

Gene Symbol

GDNF

UniProt

NP_000505.1 (Human)

Additional GDNF Products

Product Documents for Recombinant Human GDNF Protein, Animal-Free

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.  

 Production

  • All molecular biology procedures use animal-free media and dedicated labware.
  • Dedicated fermentors are utilized in committed animal-free areas.

Purification

  • Protein purification columns are animal-free.
  • Bulk proteins are filtered using animal-free filters.
  • Purified proteins are stored in animal-free containers in a dedicated cold storage room.

    Quality Assurance

    • Low Endotoxin Level.
    • No impairment of biological activity.
    • High quality product obtained under stringent conditions.
    • For ex vivo research or bioproduction, additional documentation can be provided.

    Please read our complete Animal-Free Statement

    Product Specific Notices for Recombinant Human GDNF Protein, Animal-Free

    For research use or further manufacturing only

    Related Research Areas

    GDNF Family

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