Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.
Recombinant Human GDNF Protein, CF
R&D Systems | Catalog # BT-GDNF
Key Product Details
Source
Accession Number
Structure / Form
Applications
Product Specifications
Source
Gly113-Ile211
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells.
The ED50 for this effect
is 0.750-9.00 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1
Fc Chimera (Catalog # 714‑GR).
Scientific Data Images for Recombinant Human GDNF Protein, CF
Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human GDNF.
Equivalent bioactivity of GMP (BT-GDNF-GMP), Animal-Free (BT-GDNF-AFL) and RUO (Catalog # BT-GDNF) grades of Recombinant Human GDNF as measured in a cell proliferation assay using the cell proliferation of SH-SY5Y cells (orange, green, red, respectively).
Recombinant Human GDNF Protein Bioactivity.
Recombinant Human GDNF Protein (Catalog # BT-GDNF) stimulates proliferation in the SH-SY5Y human neuroblastoma cell line. The ED50 for this effect is 0.750-9.00 ng/mL in the presence of Recombinant Human GFRa-1/GDNF Ra-1 Fc Chimera Protein (714-GR).
Recombinant Human GDNFProtein SDS-PAGE.
2 μg/lane ofRecombinant Human GDNFProtein (Catalog # BT-GDNF)was resolved with SDS-PAGEunder reducing (R) and nonreducing (NR) conditions andvisualized by Coomassie® Bluestaining, showing a band at 8 kDaunder reducing conditions.Formulation, Preparation, and Storage
BT-GDNF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute the 10 μg size at 100 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: GDNF
Long Name
Alternate Names
Gene Symbol
UniProt
Additional GDNF Products
Product Documents for Recombinant Human GDNF Protein, CF
Product Specific Notices for Recombinant Human GDNF Protein, CF
For research use only
Related Research Areas
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