Recombinant Human GDNF Protein, CF

R&D Systems | Catalog # BT-GDNF

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Key Product Details

Source

E. coli

Accession Number

NP_000505.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all GDNF Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human GDNF protein
Gly113-Ile211

Purity

>97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly113 & Met

Predicted Molecular Mass

16 kDa

SDS-PAGE

8 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells.
The ED50 for this effect is 0.750-9.00 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714‑GR).

Scientific Data Images for Recombinant Human GDNF Protein, CF

Equivalent Bioactivity of GMP, Animal-Free, and RUO grades of Recombinant Human GDNF.

Equivalent bioactivity of GMP (BT-GDNF-GMP), Animal-Free (BT-GDNF-AFL) and RUO (Catalog # BT-GDNF) grades of Recombinant Human GDNF as measured in a cell proliferation assay using the cell proliferation of SH-SY5Y cells (orange, green, red, respectively).

Recombinant Human GDNF Protein Bioactivity.

Recombinant Human GDNF Protein (Catalog # BT-GDNF) stimulates proliferation in the SH-SY5Y human neuroblastoma cell line. The ED50 for this effect is 0.750-9.00 ng/mL in the presence of Recombinant Human GFRa-1/GDNF Ra-1 Fc Chimera Protein (714-GR).

Recombinant Human GDNFProtein SDS-PAGE.

2 μg/lane ofRecombinant Human GDNFProtein (Catalog # BT-GDNF)was resolved with SDS-PAGEunder reducing (R) and nonreducing (NR) conditions andvisualized by Coomassie® Bluestaining, showing a band at 8 kDaunder reducing conditions.

Formulation, Preparation, and Storage

BT-GDNF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. 
Reconstitution Reconstitute the 10 μg size at 100 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Long Name

Glial Cell line-derived Growth Factor

Alternate Names

ATF, HFB1-GDNF, HGDNF, HSCR3

Entrez Gene IDs

2668 (Human); 25453 (Rat)

Gene Symbol

GDNF

UniProt

NP_000505.1 (Human)

Additional GDNF Products

Product Documents for Recombinant Human GDNF Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human GDNF Protein, CF

For research use only

Related Research Areas

GDNF Family

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