Recombinant Human GFR alpha-3 Fc Chimera Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human GFR alpha-3 Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Binding of R&D Systems’ human GFR alpha -3/Fc Chimera to immobilized Artemin has been reported. Baloh, R. et al. (1998) Neuron 21:1291. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Artemin (Catalog # 2589-AR) at 1 µg/mL can bind Recombinant Human GFR alpha ‑3/GDNF R alpha ‑3 Fc Chimera with an apparent Kd <1 nM.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human GFR alpha-3/GDNF R alpha-3 protein
Human GFR alpha -3
Accession # AAC24355
6 His tag
Human GFR alpha -3
Accession # AAC24355
6 His tag
Accession #
N-terminal Sequence
Gly31 & Asp32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
67 kDa (monomer)
67 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: GFR alpha-3/GDNF R alpha-3

Human glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 3 (GFR alpha -3, GDNF R alpha ‑3) is an approximately 50 kDa plasma membrane glycoprotein that is one of four GDNF receptors, all of which are glycosylphosphoinositol (GPI)‑anchored, contain three conserved cysteine repeats, and promote survival of neurons (1‑4). It is synthesized as a 400 aa precursor with a 31 aa N‑terminal signal sequence, a 343 aa mature segment, and a C-terminal GPI attachment signal sequence (3‑5). Human GFR alpha -3 shares 81‑84% aa sequence identity with mouse, rat, equine, bovine and canine GFR alpha -3. GFR alpha -3 is expressed in the central nervous system only during the earliest stages of neurogenesis, while later it is predominantly found in developing and adult nociceptive sensory neurons (2, 4‑8). It is expressed with the shared GDNF co‑receptor, the Ret receptor tyrosine kinase, in the trigeminal ganglion, pituitary gland, thymus, lung, and duodenum (2, 4). Its ligand, the GDNF family ligand artemin, is primarily expressed by immature Schwann cells in the peripheral nervous system, and vascular smooth muscle cells, directing axonal projection of sympathetic neurons (6, 7). Artemin first binds GFR alpha -3, which recruits Ret, forming a signaling complex that is a pentamer containing one artemin, two GFR alpha -3, and two Ret molecules (1, 6, 9). Signals from this complex are required for the development and survival of the superior cervical ganglion (SCG) neurons, and deletion of mouse GFR alpha -3 results in deficits in the SCG and inhibited development of intestinal Peyer’s patches (6, 7, 10, 11).

  1. Wang, X. et al. (2006) Structure 14:1083.
  2. Naveilhan, P. et al. (1998) Proc. Natl. Acad. Sci. USA 95:1295.
  3. Nomoto, S. et al. (1998) Biochem. Biophys. Res. Commun. 244:849.
  4. Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  5. Worby, C. et al. (1998) J. Biol. Chem. 273:3502.
  6. Baloh, R. et al. (1998) Neuron 21:1291.
  7. Honma, Y. et al. (2002) Neuron 35:267.
  8. Orozco, O.E. et al.(2001) Eur. J. Neurosci. 13:2177.
  9. Schlee, S. et al. (2006) Nat. Chem. Biol. 2:636.
  10. Nishino, J et al. (1999) Neuron 23:725.
  11. Veiga-Fernandez, H. et al. (2007) Nature 446:547.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 3
Entrez Gene IDs
2676 (Human); 14587 (Mouse)
Alternate Names
GDNF family receptor alpha 3; GDNF family receptor alpha-3; GDNF R alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR alpha3; GFR alpha-3; GFRA3; GFRa-3; GFR-alpha-3; glial cell line-derived neurotrophic factor receptor alpha-3; GPI-linked receptor

Citations for Recombinant Human GFR alpha-3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
    Authors: Lu B, PereiraPerrin M
    Infect. Immun., 2008-06-09;76(8):3530-8.
    Species: Bacteria
    Sample Types: Trypanosoma
    Applications: PcIP


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