Recombinant Human Glypican 2 Protein, CF

Catalog # Availability Size / Price Qty
2304-GP-050

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Recombinant Human Glypican 2 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Glypican 2 at 4 µg/mL (100 µL/well) can bind Recombinant Human Midkine (Catalog # 258-MD) with a linear range of 4-200 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Glypican 2 protein
Pro18-Ser553 & Ala359-Ser553, both with a C-terminal 6-His tag & Pro18-Ala359
Accession #
N-terminal Sequence
Analysis
Pro18 & Ala359
Predicted Molecular Mass
59.2 kDa
SDS-PAGE
50 - 60 kDa, non-reducing conditions

Product Datasheets

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2304-GP

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2304-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Glypican 2

The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35-63% sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54% sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17-25% sequence similarity (1-4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine-GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).

References
  1. Filmus, J and S.B. Selleck (2001) J. Clin. Invest. 108:497.
  2. De Cat, B and G. David (2001) Semin. Cell Dev. Biol. 12:117.
  3. Song, H. and J. Filmus (2002) Biochem. Biophys. Acta 1573:241.
  4. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
  5. Ivins, J.K. et al. (1997) Dev. Biol. 184:320.
  6. Kurosawa, N. et al. (2001) Glycoconj. J. 18:499.
Entrez Gene IDs
221914 (Human); 71951 (Mouse)
Alternate Names
2410016G05Rik; Cerebroglycan; Glypican 2; GPC2

Citations for Recombinant Human Glypican 2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Homology-independent targeted insertion (HITI) enables guided CAR knock-in and efficient clinical scale CAR-T cell manufacturing
    Authors: Balke-Want, H;Keerthi, V;Gkitsas, N;Mancini, AG;Kurgan, GL;Fowler, C;Xu, P;Liu, X;Asano, K;Patel, S;Fisher, CJ;Brown, AK;Tunuguntla, RH;Patel, S;Sotillo, E;Mackall, CL;Feldman, SA;
    Molecular cancer
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The IgG4 hinge with CD28 transmembrane domain improves VHH-based CAR T cells targeting a membrane-distal epitope of GPC1 in pancreatic cancer
    Authors: N Li, A Quan, D Li, J Pan, H Ren, G Hoeltzel, N de Val, D Ashworth, W Ni, J Zhou, S Mackay, SM Hewitt, R Cachau, M Ho
    Nature Communications, 2023-04-08;14(1):1986.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  3. Doublecortin and Glypican-2 concentrations in the cerebrospinal fluid from infants are developmentally downregulated
    Authors: C Brégère, U Fisch, FS Halbeisen, C Schneider, T Dittmar, S Stricker, S Aghlmandi, R Guzman
    PLoS ONE, 2023-02-17;18(2):e0279343.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Control
  4. Glypican-2 levels in cerebrospinal fluid predict the status of adult hippocampal neurogenesis
    Authors: S Lugert, T Kremer, R Jagasia, A Herrmann, S Aigner, C Giachino, I Mendez-Dav, AM Gardier, JP Carralot, H Meisterman, A Augustin, MD Saxe, J Lamerz, G Duran-Pach, A Ducret, V Taylor, DJ David, C Czech
    Sci Rep, 2017-04-25;7(0):46543.
    Species: Human
    Sample Types: Antibody, Whole Cells
    Applications: Bioassay, ELISA (Control)

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