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Recombinant Human GM-CSF (CHO-expressed) Protein

R&D Systems | Catalog # 7954-GM

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human GM-CSF (CHO-expressed) Protein (7954-GM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P04141

Applications

Bioactivity
View all GM-CSF Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human GM-CSF protein
Ala18-Glu144

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala18

Predicted Molecular Mass

14.5 kDa

SDS-PAGE

20-35 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 6-30 pg/mL.

Reviewed Applications

Read 7 reviews rated 4.9 using 7954-GM in the following applications:

Scientific Data Images for Recombinant Human GM-CSF (CHO-expressed) Protein

Recombinant Human GM‑CSF Protein SEC-MALS.

Recombinant Human GM-CSF (Catalog # 7954-GM) has a molecular weight (MW) of 16.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human GM-CSF (CHO-expressed) Protein Bioactivity

Recombinant Human GM-CSF (CHO-expressed) Protein Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 6-30 pg/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7954-GM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7954-GM/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GM-CSF

GM‑CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte‑macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM‑CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM‑CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM‑CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3‑5). It shows clinical effectiveness in ameliorating chemotherapy‑induced neutropenia, and GM‑CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM‑CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled alpha ‑helices (8‑12). Mature human GM‑CSF shares 63%‑70% amino acid sequence identity with canine, feline, porcine, and rat GM‑CSF and 54% with mouse GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta  chain (CD131) which is also a component of the high‑affinity receptors for IL‑3 and IL‑5 (13, 14). In addition, GM‑CSF binds a naturally occurring soluble form of GM‑CSF R alpha (15). Human GM‑CSF is active on canine and feline cells but not on murine cells (16‑18).

References

  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
  7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
  8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
  9. Diederichs, K. et al. (1991) Science 254:1779. 
  10. Cantrell, M.A. et al. (1985) Proc. Natl. Acad. Sci. 82:6250.
  11. Lee, F. et al. (1985) Proc. Natl. Acad. Sci. 82:4360.
  12. Wong, G.G. et al. (1985) Science 228:810.
  13. Onetto-Pothier, N. et al. (1990) Blood 75:59.
  14. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
  15. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  16. Hogge, G.S. et al. (1990) Cancer Gene Ther. 6:26.
  17. Sprague, W.S. et al. (2005) J. Comp. Pathol. 133:136.
  18. Shanafelt, A.B. et al. (1991) J. Biol. Chem. 266:13804.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Entrez Gene IDs

1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)

Gene Symbol

CSF2

UniProt

P04141 (Human)

Additional GM-CSF Products

Product Documents for Recombinant Human GM-CSF (CHO-expressed) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 7954-GM (with carrier)
Download SDS 7954-GM/CF (carrier free)

Product Specific Notices for Recombinant Human GM-CSF (CHO-expressed) Protein

For research use only

Citations for Recombinant Human GM-CSF (CHO-expressed) Protein

Customer Reviews for Recombinant Human GM-CSF (CHO-expressed) Protein (7)

4.9 out of 5
7 Customer Ratings
5 Stars
86%
4 Stars
14%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/18/2020
    Recombinant Human GM-CSF (CHO-expressed) Protein 7954-GM
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Jayalakshmi Miriyala
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 10/01/2020
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Robert Berahovich
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 09/29/2020
    Flow cytometric of human monocytes (left) before and after differentiation into dendritic cells (right). CD1a-CD14+ monocytes are in the upper left quadrant, whereas CD1a+CD14- dendritic cells are in the lower right quadrant.
    Recombinant Human GM-CSF (CHO-expressed) Protein 7954-GM
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 09/16/2019
    Recombinant Human GM-CSF (CHO-expressed) Protein 7954-GM
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Mahesh Raundhal
    Application: Cell differentiation
    Verified Customer | Posted 11/19/2017
    Enriched CD14+ monocytes were cultured with 500 IU/mL IL-4 and 1000 IU/mL GM-CSF for 6 days.
    Recombinant Human GM-CSF (CHO-expressed) Protein 7954-GM
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/16/2017
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: jacqueline lee
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/09/2016
    Recombinant Human GM-CSF (CHO-expressed) Protein 7954-GM

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FAQs for Recombinant Human GM-CSF (CHO-expressed) Protein

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  • Q: Is there a mass to unit conversion factor for Recombinant Human GM-CSF (CHO-expressed) Protein (Catalog # 7954-GM & 7954-GM/CF)?

    A: We have not developed a specific mass to unit conversion factor for Recombinant Human GM-CSF CHO-expressed Protein (Catalog # 7954-GM & 7954-GM/CF).  That said, because Catalog # 7954-GM and Recombinant Human GM-CSF E. coli-expressed Protein (Catalog # 215-GM) share an ED50 range for our routine QC assay (Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 6-30 pg/mL), it is possible to use the mass to unit conversion factor for Catalog # 215-GM as a starting point for Catalog # 7954-GM.  Catalog # 215-GM has been evaluated against human GM-CSF WHO International Standard (NIBSC code: 88/646).  The specific activity of Recombinant Human GM-CSF (Catalog # 215-GM) is approximately 1.5 x 104 IU/μg, which is calibrated against human GM-CSF WHO International Standard (NIBSC code: 88/646).  Since units of activity can vary per assay, we would recommend that end-users develop a conversion factor for their individual application.

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