Recombinant Human GSTM1 Protein, CF

R&D Systems | Catalog # 6894-GT

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems E. coli-derived Recombinant Human GSTM1 Protein (6894-GT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P09488

Applications

Enzyme Activity
View all Glutathione S-Transferase mu 1/GSTM1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Glutathione S-Transferase mu 1/GSTM1 protein
Met1-Lys218

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1 and Pro2

Predicted Molecular Mass

26 kDa

SDS-PAGE

25-27 kDa, reducing conditions

Activity

Measured by the conjugation of reduced glutathione to 1-bromo-2,4-dinitrobenzene.
The specific activity is >20,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation, and Storage

6894-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Glutathione S-Transferase mu 1/GSTM1

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. The gene encoding GSTM1 is mapped onto human chromosome 1p13.3 and is known to be highly polymorphic (1). Mostly notably, the widely occurring GSTM1-null genotype has been linked to a variety of cancers including lung (2), gastric (3), bladder (4) and prostate (5). In addition to its role in releasing oxidative stress, GSTM1 has also been suggested to act as a hormone binding protein and play a role in maintaining hormone homeostasis in the body (6, 7).

References

  1. Pearson, W. R. et al. (1993) Am. J. Hum. Genet. 53:220.
  2. Mohr, L.C. et al. (2003) Anticancer. Res. 23:2111.
  3. Wang, H. et al. (2010) Dig. Dis. Sci. 55:1824.
  4. Engel, L. S. et al. (2002) Am. J. Epidermiol. 156:95.
  5. Mo, Z. et al. (2009) Prostate. 69:662.
  6. Mukherjee, S. B. et al. (1999) Biochem. J. 340:309.
  7. Ishigaki, S. et al. (1989) Arch. Biochem. Biophys. 273:265.

Alternate Names

Glutathione STransferase mu 1, GST1, GSTM1, GTH4, GTM1

Entrez Gene IDs

2944 (Human)

Gene Symbol

GSTM1

UniProt

P09488

Additional Glutathione S-Transferase mu 1/GSTM1 Products

Product Documents for Recombinant Human GSTM1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human GSTM1 Protein, CF

For research use only

Customer Reviews for Recombinant Human GSTM1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human GSTM1 Protein, CF and earn rewards!

Have you used Recombinant Human GSTM1 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Recombinant Human GSTM1 Protein, CF (6894-GT):

Materials
  • Assay Buffer: 50 mM Sodium Phosphate, pH 7.0
  • Recombinant Human Glutathione S‑Transferase mu 1/GS (rhGSTM1) (Catalog # 6894-GT)
  • L-Glutathione, reduced (GSH) (Amresco, Catalog # 0399), 250 mM stock in deionized water
  • Substrate: 1-Bromo-2,4-dinitrobenzene (BDNB) (Sigma, Catalog # 262226), 75 mM stock in ethanol
  • UV plate (Costar, Catalog # 3635)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute GSH to 1 mM in Assay Buffer.
  2. Dilute rhGSTM1 to 0.2 ng/μL in Assay Buffer containing 1 mM GSH.
  3. Dilute Substrate to 2 mM in Assay Buffer containing 1 mM GSH.
  4. Load into a plate 50 μL of 0.2 ng/μL rhGSTM1 and start the reaction by adding 50 μL of 2 mM Substrate. For Substrate Blanks, load 50 μL of Assay Buffer containing 1 mM GSH and 50 μL of 2 mM Substrate.
  5. Read plate at a wavelength of 340 nm (bottom read) in kinetic mode for 5 minutes.
  6. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank 
     **Using the extinction coefficient 9600 M-1cm-1 
     ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD. Per Well:
  • rhGSTM1: 0.010 μg
  • Substrate: 1 mM
  • GSH: 1 mM

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes