Recombinant Human Guanylyl Cyclase C Fc Avi-tag Protein, CF SummaryLearn more about Avi-tag Biotinylated Proteins
|Human Guanylyl Cyclase C/GUCY2C|
Accession # P25092.2
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 1.00 mg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Human Guanylyl Cyclase C Antibody (MAB2157) is immobilized at 100 ng/mL (100 µL/well), Biotinylated Recombinant Human Guanylyl Cyclase C Fc Chimera Avi-tag (Catalog # AVI10575) binds with an ED50 of 0.500‑3.00 ng/mL.
2 μg/lane of Biotinylated Recombinant Human Guanylyl Cyclase C Fc Chimera Avi-tag Protein (Catalog # AVI10575) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-120 kDa, and 210-240 kDa, respectively.
Background: Guanylyl Cyclase C/GUCY2C
Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family and helps regulate intestinal function (1, 8). There are 7 known members of the GC family (GC-A through GC-G) and they catalyze the conversion of guanosine triphosphate to cyclic guanosine monophosphate (cGMP) and pyrophosphate (1-4). Mature human GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a pseudokinase domain and a GC catalytic domain. The ECD of mature human GUCY2C shares 70% and 72% amino acid sequence identity with the ECD of mouse and rat GUCY2C, respectively. GUCY2C is primarily expressed in intestinal epithelial cells from the duodenum to rectum and binds to the endogenous gastrointestinal hormones guanylin and uroguanylin (1-4). Activation of GUCY2C signaling mediates fluid-ion homeostasis, intestinal inflammation, and cell proliferation in a cGMP-dependent manner (5). GUCY2C is also expressed in neurons, where it plays a role in attention deficiency/hyperactive behavior (6). GUCY2C is being investigated as a treatment for several gastrointestinal disorders including irritable bowel syndrome, obesity, colorectal cancer, and chronic idiopathic constipation (7, 8). Our Avi-tag Biotinylated GUCY2C features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
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- Kim, G.W. et al. (2013) Trends Endocrinol Metab 24(4):165.
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