Recombinant Human Guanylyl Cyclase C His Avi-tag Protein, CF

R&D Systems | Catalog # AVI2157

Biotinylated /GUCY2C His-tag
R&D Systems
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human Guanylyl Cyclase C His Avi-tag Protein (AVI2157)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P25092.2

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity
View all Guanylyl Cyclase C/GUCY2C Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Guanylyl Cyclase C/GUCY2C protein
Human Guanylyl Cyclase C Protein
(Ser24-Gln430)
Accession # P25092.2		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser24

Predicted Molecular Mass

49 kDa

SDS-PAGE

75-85 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human Guanylyl Cyclase C His-tag Avi-tag (Catalog # AVI2157) binds to Human Guanylyl Cyclase C Antibody (Catalog # MAB2157) with an ED50 of 0.250-1.50 ng/mL.

Scientific Data Images for Recombinant Human Guanylyl Cyclase C His Avi-tag Protein, CF

Biotinylated Recombinant Human Guanylyl Cyclase C/GUCY2C His-tag Avi-tag Protein Binding Activity.

Biotinylated Recombinant Human Guanylyl Cyclase C His-tag Avi-tag (Catalog # AVI2157) binds to Human Guanylyl Cyclase C Antibody (MAB2157) with an ED50 of 0.250-1.50 ng/mL.

Biotinylated Recombinant Human Guanylyl Cyclase C/GUCY2C His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human Guanylyl Cyclase C/GUCY2C His-tag Avi-tag Protein (Catalog # AVI2157) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-85 kDa.

Formulation, Preparation, and Storage

AVI2157
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 µg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Guanylyl Cyclase C/GUCY2C

Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family and helps regulate intestinal function (1, 8). There are 7 known members of the GC family (GC-A through GC-G) and they catalyze the conversion of guanosine triphosphate to cyclic guanosine monophosphate (cGMP) and pyrophosphate (1 - 4). Mature human GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a pseudokinase domain and a GC catalytic domain. The ECD of mature human GUCY2C shares 70% and 72% amino acid sequence identity with the ECD of mouse and rat GUCY2C, respectively. GUCY2C is primarily expressed in intestinal epithelial cells from the duodenum to rectum and binds to the endogenous gastrointestinal hormones guanylin and uroguanylin (1-4). Activation of GUCY2C signaling mediates fluid-ion homeostasis, intestinal inflammation, and cell proliferation in a cGMP-dependent manner (5). GUCY2C is also expressed in neurons, where it plays a role in attention deficiency/hyperactive behavior (6). GUCY2C is being investigated as a treatment for several gastrointestinal disorders including irritable bowel syndrome, obesity, colorectal cancer, and chronic idiopathic constipation (7, 8). Our Avi-tag Biotinylated GUCY2C features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Arshad, N. and Visweswariah S.S. (2012) FEBS Lett. 586:2835.
  2. Potter, L.R. (2011) Cell Signal (2011) 23:1921.
  3. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
  4. Gibbons, A.V. et al. (2013) Cancer Res. 73:22.
  5. Lucas, K. et al. (2000) Pharmacol Rev. 52:375.
  6. Gong, R. et al. (2011) Science 333:1642.
  7. Waldman, S.A and Camilleri, M. (2018) Gut 67:1543.
  8. Kim, G.W. et al. (2013) Trends Endocrinol Metab 24(4):165.

Alternate Names

DIAR6, GC-C, GCC, Guanylate Cyclase C, GUC2C, GUCY2C, MUCIL, STA Receptor, StAR

Entrez Gene IDs

2984 (Human); 14917 (Mouse); 25711 (Rat)

Gene Symbol

GUCY2C

UniProt

P25092.2

Additional Guanylyl Cyclase C/GUCY2C Products

Product Documents for Recombinant Human Guanylyl Cyclase C His Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human Guanylyl Cyclase C His Avi-tag Protein, CF

For research use only

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