Recombinant Human Hexa-Ub/Ub6 WT Chains (K63-linked), CF

Catalog # Availability Size / Price Qty
UC-317-025
Product Details
Citations (1)
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Recombinant Human Hexa-Ub/Ub6 WT Chains (K63-linked), CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Ubiquitin chains vary in length, linkage, and function. K63-linked Hexa-Ubiquitin Chains (Ub6) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °C for 20 minutes prior to gel electrophoresis.
Source
E. coli-derived human Hexa-Ubiquitin protein
Accession #
Predicted Molecular Mass
52 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UC-317

Formulation 1 mg/ml (19 μM) in sterile, deionized water
Reconstitution
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
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Background: Hexa-Ubiquitin

With a predicted molecular weight of 52 kDa, hexa-Ubiquitin chains are composed of six Ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule (1). Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively (2). Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes (3-8).

Linkage specific hexa-Ub can be used to investigate the mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases, the proteasome or other proteins that contain Ubiquitin-associated domains (UBAs) or Ubiquitin-interacting motifs (UIMs). This product is formed with wild-type Ubiquitin and linkage-specific enzymes. It has been shown that the rate of Ubiquitin-substrate conjugate degradation is related to poly-Ubiquitin chain length. Tetra-Ubiquitin is the minimal unit required for recognition by the proteasome, and longer chains probably have enhanced binding to proteasomal subunits and may be more resistant to disassembly by a proteasome-associated isopeptidases.

 

References
  1. Scheffner, M. et al. (1995) Nature 373:81.
  2. Sharp, P.M. & W.-H. Li (1987) Trends Ecol. Evol. 2:328.
  3. Behrends, C. & J.W. Harper (2011) Nat. Struct. & Mol. Biol. 18:520.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Henry, A.G. et al. (2012) Dev. Cell 23:519.
  6. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  7. Wei, W. et al. (2004) Nature 428:194.
  8. Zhang, J. et al. (2012) J. Biol. Chem. 287:28646.
Entrez Gene IDs
7314 (Human)
Alternate Names
HexaUbiquitin; Hexa-Ubiquitin; Ub6

Citation for Recombinant Human Hexa-Ub/Ub6 WT Chains (K63-linked), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification Of Small Molecule TRABID Deubiquitinase Inhibitors By Computation-Based Virtual Screen.
    Authors: Shi T, Bao J, Wang N, Zheng J, Wu D
    BMC Chem Biol, 2012;12(1):4.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay

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