Recombinant Human His6-E6AP/UBE3A Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human His6-E6AP/UBE3A Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human E6AP/UBE3A protein
Met1 - Leu875 with a C-terminal 6-His tag
Accession #
Predicted Molecular Mass
102 kDa

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a solution in HEPES, NaCl and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Reconstitution Calculator

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Background: E6AP/UBE3A

Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).

  1. Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343.
  2. Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156.
  3. Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095.
  4. Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765.
  5. Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407.
  6. Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.

Long Name
Human Papillomavirus E6-associated Protein
Entrez Gene IDs
7337 (Human); 22215 (Mouse); 361585 (Rat)
Alternate Names
ANCR; ANCREPVE6AP; AS; CTCL tumor antigen se37-2; E6AP ubiquitin-protein ligase; E6AP; E6-AP; EC 6.3.2.-; EPVE6AP; FLJ26981; HPVE6A; human papilloma virus E6-associated protein; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54; UBE3A; ubiquitin protein ligase E3A; ubiquitin-protein ligase E3A


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Recombinant Human His6-E6AP/UBE3A Protein, CF
By Anonymous on 09/18/2018
Application: Enzymatic activity in vitro