Recombinant Human His6-UbcH3/Cdc34 Dominant Negative, CF

Recombinant Human His6-UbcH3/Cdc34 Dominant Negative, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: UBE2R1/CDC34

Ubiquitin-conjugating Enzyme H3 (UbcH3), also known as Cell Division Cycle 34 (Cdc34), is a member of the Ubiquitin-conjugating (E2) enzyme family. UbcH3 has a predicted molecular weight of 26 kDa. The human protein shares 98% amino acid (aa) sequence identity with the mouse and rat orthologs. In addition to an E2 catalytic core domain, UbcH3 has two Ubiquitin binding sites, UBS1, from aa 205-215, and UBS2, from aa 216-225, and a C-terminal acidic tail domain. UbcH3 is required for efficient cell cycle progression in human cells. UbcH3 interacts with the SCF(Fbw7) and SCF(Skp2) Ubiquitin ligases (E3s) to target the key cell cycle proteins Myc and p27/Kip1, respectively, for ubiquitination and degradation. Furthermore, CK2 has been shown to phosphorylate human UbcH3 on Ser203, Ser222, and Ser231 in cycling cells. The proliferation of cancer cell lines is inhibited following treatment with a human UbcH3 inhibitor, suggesting that UbcH3 also plays a significant role in cancer.

Related Research Areas

• E2 Ubiquitin/Ubl Conjugating Enzymes
• Search Ubiquitin-related Enzymes