Recombinant Human His6-USP30 Protein, CF

R&D Systems | Catalog # E-582

R&D Systems
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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human His6-USP30 Protein (E-582)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

Q70CQ3.1

Applications

Bioactivity
View all USP30 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP30 protein
Thr57 - Glu517 with a N-terminal Met and a C-terminal 6-His tag

Purity

>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

54 kDa

Activity

Reaction conditions will need to be optimized for each specific application.  We recommend an initial USP30 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555), or 200-500 nM when digesting recombinant Polyubiquitin chain substrates.

Formulation, Preparation, and Storage

E-582
Formulation Supplied as a solution in HEPES, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP30

Ubiquitin carboxyl-terminal hydrolase 30 (USP30) is a member of the C19 family of peptidases and is tethered to the outer mitochondrial surface via a trans-membrane domain. USP30 activity is antagonistic to the pro-mitophagy signal generated by the activities of E3 Ubiquitin Ligase Parkin (PARK2) and kinase PINK1, which are stimulated by mitochondrial damage. In vivo, USP30 cleaves Ubiquitin from mitochondrial surface proteins such as MIRO1, TOMM20, MFN1, and MFN2. The enzyme displays a strong preference for K6- and K11-linked Polyubiquitin chains in vitro, but has substantially lower activity on Polyubiquitin chains that have been phosphorylated with PINK1. 

References

  1. Bingol B. et al. (2014) Nature 510: 370.
  2. Cunningham C.N. et al. (2015) Nat Cell Biol. 17: 160.
  3. Thobois S. (2015) Mov. Disord. 30: 340.
  4. Wang, Y. et al. (2015) Autophagy 11: 595.
  5. Wauer T., et al. (2015) EMBO J. 34: 307.
  6. Yue, W. et al. (2014) Cell Res. 24: 482.

Long Name

Ubiquitin Specific Protease 30

Alternate Names

Deubiquitinating Enzyme 30, Ubiquitin Thioesterase 30

Entrez Gene IDs

84749 (Human); 100756 (Mouse); 304579 (Rat)

Gene Symbol

USP30

UniProt

Q70CQ3.1

Additional USP30 Products

Product Documents for Recombinant Human His6-USP30 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human His6-USP30 Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human His6-USP30 Protein, CF

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