Recombinant Human His6-USP30 Protein, CF

Catalog # Availability Size / Price Qty
E-582-050
Product Details
FAQs
Reviews

Recombinant Human His6-USP30 Protein, CF Summary

Purity
>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Reaction conditions will need to be optimized for each specific application.  We recommend an initial USP30 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555), or 200-500 nM when digesting recombinant Polyubiquitin chain substrates.

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP30 protein
Contains a C-terminal 6-His tag, Thr57 - Glu517
Accession #
Predicted Molecular Mass
54 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-582

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.2, 0.4 M NaCl, 10% Glycerol (v/v), 2 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: USP30

Ubiquitin carboxyl-terminal hydrolase 30 (USP30) is a member of the C19 family of peptidases and is tethered to the outer mitochondrial surface via a trans-membrane domain. USP30 activity is antagonistic to the pro-mitophagy signal generated by the activities of E3 Ubiquitin Ligase Parkin (PARK2) and kinase PINK1, which are stimulated by mitochondrial damage. In vivo, USP30 cleaves Ubiquitin from mitochondrial surface proteins such as MIRO1, TOMM20, MFN1, and MFN2. The enzyme displays a strong preference for K6- and K11-linked Polyubiquitin chains in vitro, but has substantially lower activity on Polyubiquitin chains that have been phosphorylated with PINK1. This recombinant protein contains amino acids 57-517 of the full-length protein, and a C-terminal 6-His tag.

References
  1. Bingol B. et al. (2014) Nature 510: 370
  2. Cunningham C.N. et al. (2015) Nat Cell Biol. 17: 160
  3. Thobois S. (2015) Mov. Disord. 30: 340
  4. Wang, Y. et al. (2015) Autophagy 11: 595
  5. Wauer T., et al. (2015) EMBO J. 34: 307
  6. Yue, W. et al. (2014) Cell Res. 24: 482
Long Name
Ubiquitin Specific Protease 30
Entrez Gene IDs
84749 (Human); 100756 (Mouse); 304579 (Rat)
Alternate Names
Deubiquitinating Enzyme 30; EC 3.1.2.15; EC 3.4.19.12; FLJ40511; MGC10702; ubiquitin carboxyl-terminal hydrolase 30; ubiquitin specific peptidase 30; ubiquitin specific protease 30; Ubiquitin Thioesterase 30; Ubiquitin thiolesterase 30; ubiquitin-specific protease 30; Ubiquitin-specific-processing protease 30; Ub-specific protease 30; USP30

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human His6-USP30 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human His6-USP30 Protein, CF and earn rewards!

Have you used Recombinant Human His6-USP30 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review