Recombinant Human His6-USP7 Catalytic Domain Protein, CF

R&D Systems | Catalog # E-518B

R&D Systems
Discontinued Product
E-518B has been discontinued. View all USP7 products.

Key Product Details

Source

E. coli

Accession Number

Q93009

Applications

Bioactivity
View all USP7 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human USP7 protein
Contains an N-terminal Met-Gly-Ser-Ser and 6-His tag
Accession # Q93009

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

41 kDa

Activity

Recombinant Human His6-USP7 Catalytic Domain is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-USP7 Catalytic Domain concentration of 1-5 nM.  A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.

Reviewed Applications

Read 1 review rated 5 using E-518B in the following applications:

Formulation, Preparation, and Storage

E-518B
Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% glycerol, 1 mM DTT.

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP7

Ubiquitin Specific Peptidase 7 (USP7), also known as Herpes Virus-associated Ubiquitin Specific Protease (HAUSP), is a widely expressed deubiquitinating enzyme belonging to the peptidase C19 family (1). It has a predicted molecular weight of 130 kDa (2). Human USP7 is 1102 amino acids (aa) in length and shares 99% aa sequence identity with the mouse and rat orthologs (2,3). USP7 consists of a cysteine peptidase core (aa 208-560) that is flanked by an N-terminal TRAF-like domain (aa 50-205) and two C-terminal protease-resistant domains (aa 622-801 and 885-1061) (2,3). USP7 can be phosphorylated at Ser18 and Ser963 and ubiquitinated at Lys869 (2,4). USP7 was initially identified as a p53-interacting protein that deubiquitinates p53, thereby stabilizing the protein and inducing p53-dependent cell growth arrest and apoptosis (5). USP7 also targets the p53 regulatory proteins MDM2, MDMX, and Daxx, the epigenetic regulator Histone 2B, and the transcription factor FoxO4 (4,6-10). Additionally, USP7 interacts with the HSV-1 immediate early protein ICP0, contributing to the stabilization and transactivation capability of ICP0 during HSV-1 infection (1,11,12).

This recombinant human protein encompasses the catalytic domain of USP7 (aa 213-548) and contains an N-terminal His6-tag.


References

    1. Everett, R.D. et al. (1997) EMBO J. 16:1519.
    2. Fernández-Montalván, A. et al. (2007) FEBS J. 274:4256.
    3. Holowaty, M.N. et al. (2003) J. Biol. Chem. 278:47753.
    4. Khoronenkova, S.V. et al. (2012) Mol. Cell 45:801.
    5. Li, M. et al. (2002) Nature 416:648.
    6. Brooks, C.L. & W. Gu (2004) Cell Cycle 3:895.
    7. Meulmeester, E. et al. (2005) Cell Cycle 4:1166.
    8. Tanga, J. et al. (2010) Biochem. Biophys. Res. Commun. 393:542.
    9. van der Knaap, J.A. et al. (2005) Mol. Cell 17:695.
    10. van der Horst, A. et al. (2006) Nat. Cell Biol. 8:1043.
    11. Everett, R.D. et al. (1999) J. Virol. 73:417.
    12. Boutell, C. et al. (2005) J. Virol. 79:12342.

    Long Name

    Ubiquitin Specific Protease 7

    Alternate Names

    HAUSP, TEF1

    Entrez Gene IDs

    7874 (Human); 252870 (Mouse); 360471 (Rat)

    Gene Symbol

    USP7

    UniProt

    Q93009

    Additional USP7 Products

    Product Documents for Recombinant Human His6-USP7 Catalytic Domain Protein, CF

    Certificate of Analysis

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    Product Specific Notices for Recombinant Human His6-USP7 Catalytic Domain Protein, CF

    For research use only

    Citations for Recombinant Human His6-USP7 Catalytic Domain Protein, CF

    Customer Reviews for Recombinant Human His6-USP7 Catalytic Domain Protein, CF (1)

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    • Recombinant Human His6-USP7 Catalytic Domain Protein, CF
      Name: Patrick Chuong
      Application: Enzymatic activity in vitro
      Verified Customer | Posted 09/20/2018

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