Recombinant Human His6-USP7 Catalytic Domain Protein, CF

Catalog # Availability Size / Price Qty
E-518B-050
Product Details
Citations (1)
FAQs
Reviews (1)

Recombinant Human His6-USP7 Catalytic Domain Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-USP7 Catalytic Domain is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-USP7 Catalytic Domain concentration of 1-5 nM.  A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.
Source
E. coli-derived human USP7 protein
Contains an N-terminal Met-Gly-Ser-Ser and 6-His tag
Accession # Q93009
Accession #
Predicted Molecular Mass
41 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-518B

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% glycerol, 1 mM DTT.

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: USP7

Ubiquitin Specific Peptidase 7 (USP7), also known as Herpes Virus-associated Ubiquitin Specific Protease (HAUSP), is a widely expressed deubiquitinating enzyme belonging to the peptidase C19 family (1). It has a predicted molecular weight of 130 kDa (2). Human USP7 is 1102 amino acids (aa) in length and shares 99% aa sequence identity with the mouse and rat orthologs (2,3). USP7 consists of a cysteine peptidase core (aa 208-560) that is flanked by an N-terminal TRAF-like domain (aa 50-205) and two C-terminal protease-resistant domains (aa 622-801 and 885-1061) (2,3). USP7 can be phosphorylated at Ser18 and Ser963 and ubiquitinated at Lys869 (2,4). USP7 was initially identified as a p53-interacting protein that deubiquitinates p53, thereby stabilizing the protein and inducing p53-dependent cell growth arrest and apoptosis (5). USP7 also targets the p53 regulatory proteins MDM2, MDMX, and Daxx, the epigenetic regulator Histone 2B, and the transcription factor FoxO4 (4,6-10). Additionally, USP7 interacts with the HSV-1 immediate early protein ICP0, contributing to the stabilization and transactivation capability of ICP0 during HSV-1 infection (1,11,12).

This recombinant human protein encompasses the catalytic domain of USP7 (aa 213-548) and contains an N-terminal His6-tag.


References
    1. Everett, R.D. et al. (1997) EMBO J. 16:1519.
    2. Fernández-Montalván, A. et al. (2007) FEBS J. 274:4256.
    3. Holowaty, M.N. et al. (2003) J. Biol. Chem. 278:47753.
    4. Khoronenkova, S.V. et al. (2012) Mol. Cell 45:801.
    5. Li, M. et al. (2002) Nature 416:648.
    6. Brooks, C.L. & W. Gu (2004) Cell Cycle 3:895.
    7. Meulmeester, E. et al. (2005) Cell Cycle 4:1166.
    8. Tanga, J. et al. (2010) Biochem. Biophys. Res. Commun. 393:542.
    9. van der Knaap, J.A. et al. (2005) Mol. Cell 17:695.
    10. van der Horst, A. et al. (2006) Nat. Cell Biol. 8:1043.
    11. Everett, R.D. et al. (1999) J. Virol. 73:417.
    12. Boutell, C. et al. (2005) J. Virol. 79:12342.
    Long Name
    Ubiquitin Specific Protease 7
    Entrez Gene IDs
    7874 (Human); 252870 (Mouse); 360471 (Rat)
    Alternate Names
    Deubiquitinating enzyme 7; EC 3.1.2.15; EC 3.4.19.12; HAUSP; HAUSPTEF1; Herpes virus-associated ubiquitin-specific protease; Herpesvirus-associated ubiquitin-specific protease; TEF1; ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific peptidase 7 (herpes virus-associated); ubiquitin specific protease 7 (herpes virus-associated); Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7; USP7

    Citation for Recombinant Human His6-USP7 Catalytic Domain Protein, CF

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    1 Citation: Showing 1 - 1

    1. CHIP E3 ligase mediates proteasomal degradation of the proliferation regulatory protein ALDH1L1 during the transition of NIH3T3 fibroblasts from G0/G1 to S-phase
      Authors: QA Khan, P Pediaditak, Y Malakhau, A Esmaeilnia, Z Ashkavand, V Sereda, NI Krupenko, SA Krupenko
      PLoS ONE, 2018;13(7):e0199699.
      Species: Human
      Sample Types: Cell Lysates
      Applications: Bioassay

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    Recombinant Human His6-USP7 Catalytic Domain Protein, CF
    By Anonymous on 09/20/2018
    Application: Enzymatic activity in vitro
    Reason for Rating: Works as expected, quick shipping