Recombinant Human HSP10/EPF Protein, CF

Catalog # Availability Size / Price Qty
AP-150-050
Product Details
Citations (1)
FAQs
Reviews (1)

Recombinant Human HSP10/EPF Protein, CF Summary

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity

HSP10/HSPE1 is a molecular chaperone that assists in the folding of nascent polypeptides and the refolding of denatured proteins.  Reaction conditions will need to be optimized for each specific application.  IMPORTANT: HSP10/HSPE1 works in conjunction with HSP60/HSPD1 (Catalog # AP-140) and both proteins are required for enzymatic activity.   For in vitro use we recommend an initial HSP60/HSPD1 concentration of 2-3 μM, and HSP10/HSPE1 concentration equimolar (or above) to HSP60/HSPD1.

Source
E. coli-derived human HSP10/EPF protein
Accession # P61604
Accession #
Predicted Molecular Mass
11 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AP-150

Formulation

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: HSP10/EPF

HSP10 (also known as Chaperonin 10) is the eukaryotic homologue of the prokaryotic GroES chaperones. This protein is found mainly in mitochondria, but can also be detected in cytosol and extracellular fluids including peripheral blood. Together with HSP60 (also known as Chaperonin 60), HSP10 plays an essential role in the translocation and refolding of proteins from the cytosol into the mitochondrial matrix. Under physiological conditions, HSP60 creates two stacked heptameric rings that form a pair of central hydrophobic cavities. After an unfolded substrate protein enters one of the cavities it is capped by a heptameric HSP10 complex, thereby trapping the unfolded protein. Structural rearrangement of the substrate-containing cavity is effected via HSP60-mediated ATP hydrolysis; this changes the lining of the cavity from hydrophobic to hydrophilic and helps promote refolding of the substrate protein. Binding of ATP to HSP60 subunits on the distal ring of the complex then causes the dissociation of the HSP10 cap complex and concomitant release of the substrate protein from the proximal cavity. If the protein is not completely folded, it can be further processed by the HSP60/HSP10 complex, or can interact with other chaperoning systems.

References
  1. Cappello F, et al. (2008) Cancer Biol. Therapy 7: 801-809
  2. Hartl F.U. & Hayer-Hartl M. (2009) Nat. Struc. Mol. Biol. 16: 574-581
Long Name
Heat Shock 10 kDa Protein 1
Entrez Gene IDs
3336 (Human); 69253 (Mouse)
Alternate Names
10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; Chaperonin 10 Homolog; Chaperonin 10; CPN10HSP10; Early-pregnancy factor; EPF; GroES; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1 (chaperonin 10); HSP10; HSPE1

Citation for Recombinant Human HSP10/EPF Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A small molecule mitigates hearing loss in a mouse model of Usher syndrome III
    Authors: KN Alagramam, SR Gopal, R Geng, DH Chen, I Nemet, R Lee, G Tian, M Miyagi, KF Malagu, CJ Lock, WR Esmieu, AP Owens, NA Lindsay, K Ouwehand, F Albertus, DF Fischer, RW Bürli, AM MacLeod, WE Harte, K Palczewski, Y Imanishi
    Nat Chem Biol, 2016;0(0):.
    Species: Porcine
    Sample Types: Recombinant Protein
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human HSP10/EPF Protein, CF

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Recombinant Human HSP10/EPF Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Recombinant Human HSP10/EPF Protein, CF
By Anonymous on 08/17/2018
Application: In vitro bioactivity in cell culture