Recombinant Human HTRA1 Protein, CF

R&D Systems | Catalog # 2916-SE

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human HTRA1 Protein (2916-SE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q92743

Applications

Enzyme Activity
View all HTRA1/PRSS11 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human HTRA1/PRSS11 protein
Gly156-Pro480, with an N-terminal Met and C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

36 kDa

SDS-PAGE

37-40 kDa, reducing conditions

Activity

Measured by its ability to cleave beta -casein.
>95% of beta -Casein is cleaved by Recombinant Human HTRA1/PRSS11, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 2916-SE in the following applications:

Formulation, Preparation, and Storage

2916-SE
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: HTRA1/PRSS11

HTRA1 is a member of the mammalian HTRA (high temperature requirement A) serine protease family. The E. coli HTRA homolog functions as a chaperone-protease and is essential for bacterial survival at temperatures above 42 °C (1). Among the four mammalian HTRA proteins, HTRA1, -3 and -4 are secreted while HTRA2 is localized in mitochondria. HTRA1 contains an N-terminal insulin-like growth factor binding protein (IGFBP) domain, a Kazal-type trypsin inhibitor motif, and C-terminal trypsin-like protease and PDZ domains (2). It is involved in several pathologies including Alzheimer’s disease (3), rheumatoid arthritis (4), osteoarthritis (5) and age‑related macular degeneration (6), although the mechanisms by which HTRA1 exerts its effects are not clear. HTRA1 also has properties of a tumor suppressor protein, which makes it a target for cancer therapy (7). In addition HTRA1 is known to regulate the TGF-beta signaling pathway and bone mineralization (8, 9). Recombinant human HTRA1 lacks the N-terminal IGFBP and Kazal-like domains, but retains the serine protease and PDZ domains.

References

  1. Jiang, J. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:11939.
  2. Murwantoko et al. (2004) Biochem. J. 381:895.
  3. Grau, S. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:6021.
  4. Grau, S. et al. (2006) J. Biol. Chem. 281:6124.
  5. Hu, S. et al. (1998) J. Biol. Chem. 273:34406.
  6. Dewan, A. et al. (2006) Science 314:989.
  7. Chien, J. et al. (2004) Oncogene 23:1636.
  8. Oka, C. et al. (2004) Development 131:1041.
  9. Hadfield, K.D. et al. (2008) J. Biol. Chem. 283:5928.

Long Name

High Temperature Requirement Protein-1

Alternate Names

IGFBP5-protease, PRSS11

Entrez Gene IDs

5654 (Human)

Gene Symbol

HTRA1

UniProt

Q92743

Additional HTRA1/PRSS11 Products

Product Documents for Recombinant Human HTRA1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human HTRA1 Protein, CF

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Recombinant Human HTRA1 Protein, CF

Customer Reviews for Recombinant Human HTRA1 Protein, CF (1)

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Protocols

View specific protocols for Recombinant Human HTRA1 Protein, CF (2916-SE):

Materials
  • Assay Buffer: 50 mM Tris, pH 8.0
  • Recombinant Human HTRA1/PRSS11 (rhHTRA1) (Catalog # 2916-SE)
  • beta -Casein (Sigma, Catalog # C6905), 1 mg/mL in 25 mM Tris, 150 mM NaCl, pH 7.5
  • Reducing SDS-PAGE Sample Buffer
  • SDS-PAGE or Western Blot
  1. Dilute beta -Casein to 100 µg/mL in Assay Buffer.
  2. Dilute rhHTRA1 to 10 µg/mL in Assay Buffer.
  3. In a vial, combine equal volumes of diluted beta -Casein and diluted rhHTRA1. Also prepare two beta -Casein controls containing Assay Buffer in place of rhHTRA1.
  4. Incubate reaction and one of the controls at 37 °C for 30 minutes. Keep the other control frozen at -20 °C.
  5. After incubation, combine 25 µL of the reaction and control with 25 µL of reducing SDS-PAGE gel buffer. Mix and heat samples at 95‑100 °C for 3-5 minutes.
  6. Load 40 µL per lane and analyze the cleavage by SDS-PAGE followed by protein staining and/or Western blot.
Per Lane:
  • rhHTRA1: 0.1 µg
  • beta -Casein: 1 µg

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