Recombinant Human HTRA2/Omi Protein, CF

R&D Systems | Catalog # 1458-HT

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems E. coli-derived Recombinant Human HTRA2/Omi Protein (1458-HT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

O43464

Applications

Enzyme Activity
View all HTRA2/Omi Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human HTRA2/Omi protein
Ala134-Glu458, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

N-terminal Sequence Analysis

Ala134

Predicted Molecular Mass

36 kDa

SDS-PAGE

38 kDa, reducing conditions

Activity

Measured by its ability to cleave beta -casein.
>95% cleavage of beta -casein, as measured under the described conditions.

Formulation, Preparation, and Storage

1458-HT
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: HTRA2/Omi

HtrA2/Omi is the mammalian homologue of bacterial high temperature requirement protein (HtrA). HtrA2/Omi localizes to the mitochondria and is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. HtrA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition. This effect can be measured by reversing XIAP-BIR2 (Catalog # http://www.rndsystems.com/product_results.aspx?k=786-XB">786-XB) inhibition of Caspase-7 (Catalog # http://www.rndsystems.com/product_results.aspx?k=823-C7">823-C7) cleavage of a fluorogenic peptide (DEVD-AFC, MP Bio, Catalog # AFC-138). IC50 values for this effect are typically between 0.2 and 1.5 μM. HtrA2/Omi is trimeric and functions as a serine protease. The serine protease activity may play a more central role in apoptosis than its IAP antagonizing function. A PDZ domain regulates the serine protease activity by blocking access to the active site. The specificity of the protease is yet to be defined and no endogenous substrates are known to date.

References

  1. Suzuki, Y. et al. (2001) Mol. Cell. 8:613.
  2. van Loo, G. et al. (2002) Cell Death & Diff. 9:20.
  3. Hedge, R. et al. (2001) J. Biol. Chem. 277:432.
  4. Verhagen, A. et al. (2001) J. Biol. Chem. 277:445.
  5. Martins, L. et al. (2002) J. Biol. Chem. 277:439.
  6. Silke, J., and A. Verhagen (2002) Cell Death & Diff. 9:362.
  7. Savopoulos, J. et al. (2000) Protein Expression & Purification 19:227.

Long Name

High Temperature Requirement Protein-2

Alternate Names

Omi, PRSS25

Entrez Gene IDs

27429 (Human); 64704 (Mouse)

Gene Symbol

HTRA2

UniProt

O43464

Additional HTRA2/Omi Products

Product Documents for Recombinant Human HTRA2/Omi Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human HTRA2/Omi Protein, CF

For research use only

Citations for Recombinant Human HTRA2/Omi Protein, CF

Customer Reviews for Recombinant Human HTRA2/Omi Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human HTRA2/Omi Protein, CF and earn rewards!

Have you used Recombinant Human HTRA2/Omi Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Recombinant Human HTRA2/Omi Protein, CF (1458-HT):

Materials
  • Assay Buffer: 50 mM Tris, pH 8.0
  • Recombinant Human HTRA2/Omi (rhHTRA2) (Catalog # 1458-HT)
  • Substrate: beta -Casein (Sigma, Catalog # C-6905), 1.0 mg/mL stock in 25 mM Tris, 0.15 M NaCl, pH 7.5
  • SDS-PAGE and silver staining reagents
  1. Dilute Substrate to 0.4 mg/mL in Assay Buffer.
  2. Dilute rhHTRA2 to 0.04 mg/mL in Assay Buffer.
  3. Add 25 μL of substrate and 25 μL of rhHTRA2 to reaction tube. Include a control with 25 μL Substrate and 25 μL Assay Buffer.
  4. Incubate the reaction and control for 1 hour at 45 °C.
  5. Stop the reaction by adding SDS-PAGE sample buffer.
  6. Analyze the cleavage by SDS-PAGE followed by silver staining.
Per Reaction:
  • rhHTRA2: 0.02 mg/mL
  • Substrate: 0.2 mg/mL

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes

Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail