Recombinant Human IgG1 Fc Avi-tag Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI110-050
Recombinant Human IgG1 Fc Avi-tag Protein, CF Binding Activity
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human IgG1 Fc Avi-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When Biotinylated Recombinant Human IgG1 Fc Avi-tag (Catalog # AVI110) is immobilized at 2 µg/mL (100 µL/well) onto a Streptavidin coated plate (Catalog # CP004), it binds to Human C1q with an ED50 of 0.2-2.0 μg/mL.

Source
Human embryonic kidney cell, HEK293-derived human IgG1 protein
IEGRMD Human IgG1
(Pro100-Lys330)
Avi-tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ile
Structure / Form
Disulfide-linked homodimer, Biotinylated via Avi-tag
Predicted Molecular Mass
28 kDa
SDS-PAGE
30-42 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI110

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Biotinylated Recombinant Human IgG1 Fc Avi-tag (Catalog # AVI110) is immobilized at 2 μg/mL (100 μL/well) onto a Streptavidin coated plate (Catalog # CP004), it binds to Human C1q with an ED50 of 0.2‑2.0 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human IgG1 Fc Avi-tag (Catalog # AVI110) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-42 kDa and 60-80 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IgG1

Human Fc is the crystallizable fragment in the constant region of the human immunoglobulin heavy chain. Members of the immunoglobulin superfamily (IgSF) consist of two heavy (H) and two light (L) chains, with each component containing one variable (V) and one or more constant (C) domains (1, 2). Produced by B lymphocytes, Ig gamma (IgG) is the predominant isotype found in the body and has the longest half-life of all immunoglobulin isotypes (3). Four IgG subclasses (IgG1-4) were identified based on structural, antigenic and functional differences in the constant region of the heavy chain. The constant tail of the antibody is where proteins involved in immune responses typically bind, most notably the first component of the complement pathway, C1q, and Fc receptors found on immune cells such as B lymphocytes. Affinities for C1q and Fc gamma receptor (Fc gamma R) differ among the IgG subclasses. Fc receptors mediate the recruitment of immune cells to a site of infection (4). Engineered crystallizable fragment (Fc) regions of antibody which assume a unique and unprecedented asymmetric structure within the homodimeric Fc polypeptide, enable completely selective binding to the complement component C1q and activation of complement via the classical pathway without any concomitant engagement of the Fc gamma R (5).

References
  1. Williams, A.F. and A.N. Barclay (1988) Annu. Rev. Immunol. 6:381.
  2. Harpaz, Y. and C. Chothia (1994) J. Mol. Biol. 238:528.
  3. Schroeder, H.W. and L. Cavacini (2010) J. Allergy Clin. Immunol. 125:S41.
  4. Duncan, A.R. and G. Winter (1998) Nature 332:738.
  5. Lee, C.H. et al. (2017) Nat. Immunol. 18:889.
Long Name
Immunoglobulin G1
Alternate Names
IgG1

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human IgG1 Fc Avi-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human IgG1 Fc Avi-tag Protein, CF and earn rewards!

Have you used Recombinant Human IgG1 Fc Avi-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review