Recombinant Human IgG1 Fc Avi-tag Protein, CF

R&D Systems | Catalog # AVI110

Biotinylated
R&D Systems
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human IgG1 Fc Avi-tag Protein (AVI110)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P01857.1

Structure / Form

Disulfide-linked homodimer, Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IgG1 protein
IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile

Predicted Molecular Mass

28 kDa

SDS-PAGE

30-42 kDa

Activity

Measured by its binding ability in a functional ELISA.

When Biotinylated Recombinant Human IgG1 Fc Avi-tag (Catalog # AVI110) is immobilized at 2 µg/mL (100 µL/well) onto a Streptavidin coated plate (Catalog # CP004), it binds to Human C1q with an ED50 of 0.2-2.0 μg/mL.

Reviewed Applications

Read 1 review rated 5 using AVI110 in the following applications:

Scientific Data Images for Recombinant Human IgG1 Fc Avi-tag Protein, CF

Recombinant Human IgG1 Fc Avi-tag Protein Binding Activity

Recombinant Human IgG1 Fc Avi-tag Protein Binding Activity

When Biotinylated Recombinant Human IgG1 Fc Avi-tag (Catalog # AVI110) is immobilized at 2 µg/mL (100 µL/well) onto a Streptavidin coated plate (Catalog # CP004), it binds to Human C1q with an ED50 of 0.2-2.0 µg/mL.
Recombinant Human IgG1 Fc Avi-tag Protein SDS-PAGE

Recombinant Human IgG1 Fc Avi-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human IgG1Fc Avi-tag (Catalog # AVI110) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-42 kDa and 60-80 kDa, respectively.

Formulation, Preparation, and Storage

AVI110
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG1

Human Fc is the crystallizable fragment in the constant region of the human immunoglobulin heavy chain. Members of the immunoglobulin superfamily (IgSF) consist of two heavy (H) and two light (L) chains, with each component containing one variable (V) and one or more constant (C) domains (1, 2). Produced by B lymphocytes, Ig gamma (IgG) is the predominant isotype found in the body and has the longest half-life of all immunoglobulin isotypes (3). Four IgG subclasses (IgG1-4) were identified based on structural, antigenic and functional differences in the constant region of the heavy chain. The constant tail of the antibody is where proteins involved in immune responses typically bind, most notably the first component of the complement pathway, C1q, and Fc receptors found on immune cells such as B lymphocytes. Affinities for C1q and Fc gamma receptor (Fc gamma R) differ among the IgG subclasses. Fc receptors mediate the recruitment of immune cells to a site of infection (4). Engineered crystallizable fragment (Fc) regions of antibody which assume a unique and unprecedented asymmetric structure within the homodimeric Fc polypeptide, enable completely selective binding to the complement component C1q and activation of complement via the classical pathway without any concomitant engagement of the Fc gamma R (5).

References

  1. Williams, A.F. and A.N. Barclay (1988) Annu. Rev. Immunol. 6:381.
  2. Harpaz, Y. and C. Chothia (1994) J. Mol. Biol. 238:528.
  3. Schroeder, H.W. and L. Cavacini (2010) J. Allergy Clin. Immunol. 125:S41.
  4. Duncan, A.R. and G. Winter (1998) Nature 332:738.
  5. Lee, C.H. et al. (2017) Nat. Immunol. 18:889.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

UniProt

P01857.1

Additional IgG1 Products

Product Documents for Recombinant Human IgG1 Fc Avi-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human IgG1 Fc Avi-tag Protein, CF

For research use only

Citations for Recombinant Human IgG1 Fc Avi-tag Protein, CF

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  • Recombinant Human IgG1 Fc Avi-tag Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 01/06/2026
    Always work well as a control protein in my assays. Binding is detected after immobilizing the protein on a plate.
    Recombinant Human IgG1 Fc Avi-tag Protein, CF AVI110

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