Recombinant Human IgLON5 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9728-IG-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human IgLON5 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human IgLON5 Fc Chimera, immobilized at 2.5-5 μg/mL, is able to significantly enhance neurite outgrowth.
Source
Chinese Hamster Ovary cell line, CHO-derived human IgLON5 protein
Human IGLON-5
(Leu33-Asn314)
Accession # A6NGN9
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus
C-terminus
Accession #
N-terminal Sequence
Analysis
Leu33
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
58 kDa 
SDS-PAGE
77-92 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9728-IG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgLON5

IGLON family member 5 (IgLON5) is a member of the IgLON (LSAMP, OPCML/OBCAM, neurotrimin) family of neuronal cell adhesion molecules which, in turn, are part of the much larger Ig domain-containing superfamily (1). The overall structure of IgLON proteins contain three immunoglobulin domains, with numerous potential glycosylation sites, followed by a glycosylphosphatidylinositol (GPI) anchor (2). Mature human IgLON5 is 306 amino acids (aa) in length and shares 98% aa identity with both mouse and rat. Although the specific function of IgLON5 has yet to be elucidated, IgLON proteins are known to form homophilic and heterophilic complexes along the cell surface and several have been shown to shed from mature cortical neurons in a metalloproteinase-dependent manner. These go on to play a role in processes such as neuronal differentiation and survival, axon guidance and outgrowth and cell migration (3, 4). IgLON-5 is expressed in both neurons and oligodendrocytes (5). In the brain, the level of Iglon5 transcript is highest in the brainstem, thalamus and eye, and weakest in the hippocampus, septum, and pituitary gland (6). IgLON-5 is also expressed in non-neural organs and tissue types where it may function as a tumor-suppressor (6-7). Newly classified anti-IgLON5 syndrome is a novel neuronal tauopathy where antibodies target surface IgLON5, causing an irreversible internalization of the protein, manifesting as sleep dysfunction (8-10).

References
  1. Sellar G, et al. (2003) Nat. Genet. 34(3):337.
  2. Itoh S, et al. (2008) Biochemistry 47(38):10132.
  3. Sanz R, et al. (2015) J. Biol. Chem. 290(7):4330.
  4. Karagogeos D (2003) Front. Biosci. 8:1304.
  5. Sharma, K. et. al. (2015) Nat. Neurosci. 18:1819.
  6. Vanaveski, T. et. al. (2017) Front. Neurosci. 11:Article 38.
  7. Baroy, T. et. al. (2014) Mol. Cancer 13:139.
  8. Sabater L, et al. (2014) Lancet Neurol. 13(6):575.
  9. Sabater L, et al. (2016) J. Neuroinflamm. 13:226.
  10. Gelpi E, et al. (2016) Acta Neuropathol. 132:531.
Long Name
IgLON Family Member 5
Entrez Gene IDs
402665 (Human); 210094 (Mouse); 308557 (Rat)
Alternate Names
hCG1651476; Ig-like domain-containing protein ENSP00000270642; IgLON family member 5; IgLON5

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