Recombinant Human IGSF4B/SynCAM3 Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Supplemental Products

Recombinant Human IGSF4B/SynCAM3 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 50 ng on a nitrocellulose-coated microplate.
Mouse myeloma cell line, NS0-derived human IGSF4B/SynCAM3 protein
Pro21-Tyr329 & Asn25-Tyr329, both with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Pro21 & Asn25
Predicted Molecular Mass
34.5 kDa
38-41 kDa, reducing conditions

Complete Your Experiment

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: IGSF4B/SynCAM3

IGSF4B (immunoglobulin superfamily member 4B), also called CADM3, TSLL1, Necl-1 and SynCAM-3, is a neural tissue-specific member of the nectin-like family of immunoglobulin superfamily (1, 2). It is a 48 - 50 kDa type I transmembrane (TM) glycoprotein that is concentrated at non-junctional contact sites of neuronal axons and glial processes (2, 3). In myelinated peripheral nerve fibers, IGSF4B is concentrated at nodes of Ranvier in contact sites of Schwann cells (2). The 398 amino acid (aa) human IGSF4B contains a 24 aa signal sequence, a 306 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 47 aa cytoplasmic domain. The ECD contains an N-terminal V-type Ig-like domain that is responsible for Ca++‑independent homophilic and heterophilic interactions with Nectin-1, Nectin-3 or Necl-2 (IGSF-4) in trans. It also contains two C2‑type Ig-like domains that are responsible for Ca++-independent homophilic dimerization in cis that is thought to precede trans interaction (2, 4, 5). The cytoplasmic domain binds members of the MAGUK guanylate kinase subfamily, such as Dlg3, Pals2 and CASK (2). These activities are thought to play roles in adhesion and architecture at the synapse (2). We have also shown that IGSF4B promotes in vitro outgrowth of cortical neurons. Of two known splice variants, one shows an insertion of 34 aa near the mature N-terminus and probably represents a 60 kDa form. A smaller variant shows deletions of aa 185 - 231, and 281 - 398, which includes the first C2‑type Ig-like domain and the TM segment. The IGSF4B ECD is highly conserved, sharing 95 - 96% aa identity between human mouse, rat, canine and bovine sequences. The ECD of IGSF4A, B, C and D proteins share 35 - 50% aa identity.

  1. Fukuhara, H. et al. (2001) Oncogene 20:5401.
  2. Kakunaga, S. et al. (2005) J. Cell Sci. 118:1267.
  3. Fukami, T. et al. (2003) Gene 323:11.
  4. Shingai, T. et al. (2003) J. Biol. Chem. 278:35421
  5. Dong, X. et al. (2006) J. Biol. Chem. 281:10610.
Long Name
Immunoglobulin Superfamily, Member 4B
Entrez Gene IDs
57863 (Human); 94332 (Mouse); 360882 (Rat)
Alternate Names
BIgR; Brain immunoglobulin receptor; CADM3; cell adhesion molecule 3; IGSF4B; IGSF4BSynCAM3; Immunoglobulin superfamily member 4B; member 4B; Necl-1; NECL1TSLC1-like protein 1; Nectin-like protein 1; Synaptic cell adhesion molecule 3; SynCAM3; TSLC1-like 1; TSLL1; TSLL1SYNCAM3


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human IGSF4B/SynCAM3 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human IGSF4B/SynCAM3 Protein, CF and earn rewards!

Have you used Recombinant Human IGSF4B/SynCAM3 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review