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Recombinant Human IL-11 Protein

Catalog #: 218-IL
23 Citations Datasheet / COA / SDS

Bigger Scale, More Savings. Try our NEW version of Human Recombinant IL-11 (10836-IL). Combining R&D Systems quality with scalability that allows for lower price points and a solid supply chain.

Carrier Free

Catalog # Availability Size / Price Qty
218-IL-005/CF
218-IL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
218-IL-005
218-IL-025
Recombinant Human IL-11 Protein Bioactivity
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Product Details
Citations (23)
FAQs
Supplemental Products
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-11 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.02-0.12 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-11 protein
Pro22-Leu199
Accession #
P20809.1
N-terminal Sequence
Analysis
Pro22
Predicted Molecular Mass
19 kDa
SDS-PAGE
23 kDa, reducing conditions

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Recombinant Human IL-11, ACFP Protein
Cat # ACFP218

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218-IL (with carrier)

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SDS

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218-IL/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

218-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

218-IL/CF

Formulation Supplied as a 0.2 μm filtered solution in PBS and EDTA.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Scientific Data

Bioactivity Recombinant Human IL-11 Protein Bioactivity View Larger

Recombinant Human IL-11 (Catalog # 218‑IL) stimulates cell proliferation of the T11 mouse plasmacytoma cell line. The ED50 for this effect is 0.02-0.12 ng/mL.

SDS-PAGE Recombinant Human IL-11 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human IL-11 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-11

IL-11 (Interleukin 11) is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27 and IL-31 (1-3). In humans, IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF) (3). The human IL-11 cDNA encodes a 199 amino acid (aa) precursor, which generates a 178 aa, 19 kDa mature unglycosylated protein. Mature human IL-11 shares 88%, 88%, and 96% aa sequence identity with mouse, rat and canine IL-11, respectively. IL-11 is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts, among other cell types (1). It is found in the plasma mainly during inflammation, such as that associated with viral infection, cancer, or inflammatory arthritis, and is considered to be primarily anti‑inflammatory (1). It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine (1). It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis (1). IL-11 synergizes with several other cytokines to produce these effects, and its effects overlap with those of IL-6 (1). IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 R alpha subunit and two molecules of the ubiquitously expressed cell signaling beta subunit, gp130 (4). A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface, or inhibit cell surface IL-11 R alpha /gp130 signaling (5-7).

References
  1. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
  2. Paul, S.R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7512.
  3. Kawashima, I. et al. (1991) FEBS Lett. 283:199.
  4. Barton, V.A. et al. (2000) J. Biol. Chem. 275:36197.
  5. Curtis, D.J. et al. (1997) Blood 90:4403.
  6. Baumann, H. et al. (1996) J. Immunol. 157:284.
  7. Karow, J. et al. (1996) Biochem. J. 318:489.
Long Name
Interleukin 11
Entrez Gene IDs
3589 (Human); 16156 (Mouse); 171040 (Rat); 102128313 (Cynomolgus Monkey)
Alternate Names
Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin

Citations for Recombinant Human IL-11 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis
    Authors: S Xu, X Yang, Q Chen, Z Liu, Y Chen, X Yao, A Xiao, J Tian, L Xie, M Zhou, Z Hu, F Zhu, X Xu, F Hou, J Nie
    EBioMedicine, 2022;86(0):104312.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. HIRA, a DiGeorge Syndrome Candidate Gene, Confers Proper Chromatin Accessibility on HSCs and Supports All Stages of Hematopoiesis
    Authors: C Chen, MA Sun, C Warzecha, M Bachu, A Dey, T Wu, PD Adams, T Macfarlan, P Love, K Ozato
    Cell Rep, 2020;30(7):2136-2149.e4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Modeling of Fibrotic Lung Disease Using 3D Organoids Derived from Human Pluripotent Stem Cells
    Authors: A Strikoudis, A Cie?lak, L Loffredo, YW Chen, N Patel, A Saqi, DJ Lederer, HW Snoeck
    Cell Rep, 2019;27(12):3709-3723.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies
    Authors: B Mair, J Tomic, SN Masud, P Tonge, A Weiss, M Usaj, AHY Tong, JJ Kwan, KR Brown, E Titus, M Atkins, KSK Chan, L Munsie, A Habsid, H Han, M Kennedy, B Cohen, G Keller, J Moffat
    Cell Rep, 2019;27(2):599-615.e12.
    Species: Human
    Sample Types: cell culture
    Applications: Differentiation
  5. KDM3A histone demethylase functions as an essential factor for activation of JAK2-STAT3 signaling pathway
    Authors: H Kim, D Kim, SA Choi, CR Kim, SK Oh, KE Pyo, J Kim, SH Lee, JB Yoon, Y Zhang, SH Baek
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. BMTP-11 is active in preclinical models of human osteosarcoma and a candidate targeted drug for clinical translation
    Authors: VO Lewis, E Devarajan, M Cardó-Vila, DG Thomas, ES Kleinerman, S Marchiò, RL Sidman, R Pasqualini, W Arap
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes
    Immunology, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. A Novel Small-Molecule Inhibitor Targeting the IL-6 Receptor beta Subunit, Glycoprotein 130.
    Authors: Hong S, Choi J, Lee S, Park Y, Park K, Lee J, Kim J, Gajulapati V, Goo J, Singh S, Lee K, Kim Y, Im S, Ahn S, Rose-John S, Heo T, Choi Y
    J Immunol, 2015;195(1):237-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. MTA2 enhances colony formation and tumor growth of gastric cancer cells through IL-11.
    Authors: Zhou C, Ji J, Cai Q, Shi M, Chen X, Yu Y, Zhu Z, Zhang J
    BMC Cancer, 2015;15(0):343.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Expression of a surface antigen (MA6) by peripheral blood CD34+ cells is correlated with improved platelet engraftment and may explain delayed platelet engraftment following cord blood transplantation.
    Authors: Simmons P, Robinson S, Munsell M, Thomas M, Javni J, Brouard N, Zweidler-McKay P, Shpall E
    Stem Cells Dev, 2015;24(9):1066-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Regulation of IL-17A responses in human airway smooth muscle cells by Oncostatin M.
    Authors: Kwofie K, Scott M, Rodrigues R, Guerette J, Radford K, Nair P, Richards C
    Respir Res, 2015;16(0):14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
    Authors: Gori J, Butler J, Chan Y, Chandrasekaran D, Poulos M, Ginsberg M, Nolan D, Elemento O, Wood B, Adair J, Rafii S, Kiem H
    J Clin Invest, 2015;125(3):1243-54.
    Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis.
    Authors: Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M
    Blood, 2014;124(9):1393-403.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Autocrine production of IL-11 mediates tumorigenicity in hypoxic cancer cells.
    Authors: Onnis, Barbara, Fer, Nicole, Rapisarda, Annamari, Perez, Victor S, Melillo, Giovanni
    J Clin Invest, 2013;123(4):1615-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
    Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
    Int. J. Cancer, 2009;125(2):367-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression.
    Authors: Brechter AB, Lerner UH
    Arthritis Rheum., 2007;56(3):910-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells.
    Authors: Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, Zini R, Salati S, Tagliafico E, Manfredini R, Grande A, Ferrari S
    Cell Death Differ., 2006;13(10):1686-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K.
    Authors: Mandelin J, Li TF, Hukkanen M, Liljestrom M, Salo J, Santavirta S, Konttinen YT
    J. Rheumatol., 2005;32(4):713-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004;173(5):3482-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Combinations of cytokines promote survival of mice and limit acute radiation damage in concert with amelioration of vascular damage.
    Authors: Van der Meeren A, Mouthon MA, Vandamme M, Squiban C, Aigueperse J
    Radiat. Res., 2004;161(5):549-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  21. Prospective isolation of human clonogenic common myeloid progenitors.
    Authors: Manz MG, 107325, Miyamoto T, Akashi K, Weissman IL
    Proc. Natl. Acad. Sci. U.S.A., 2002;99(18):11872-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Expression of interleukin (IL)-11 receptor by the human endometrium in vivo and effects of IL-11, IL-6 and LIF on the production of MMP and cytokines by human endometrial cells in vitro.
    Authors: Cork BA, 146, Tuckerman EM, Li TC, Laird SM
    2002;8(9):841-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Interleukin-11 induces Th2 polarization of human CD4(+) T cells.
    Authors: Curti A, Ratta M, Corinti S, Girolomoni G, Ricci F, Tazzari P, Siena M, Grande A, Fogli M, Tura S, Lemoli RM
    Blood, 2001;97(9):2758-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Is the stability of this protein affected when the solution is stored frozen without carrier (BSA)?

    • The stability of this product after undergoing a freeze thaw cycle may be affected as a result of the phase change, and as such we only recommend storing the carrier-free protein at 2-8 °C.

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Animal Component Free Recombinant Proteins

Recombinant Human IL-11, ACFP Protein

ACFP218
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