Recombinant Human IL-11 Protein

Formulations:
Catalog # Availability Size / Price Qty
218-IL-005
218-IL-025
Recombinant Human IL-11 Protein Data
2 Images
Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human IL-11 Protein Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.02-0.12 ng/mL.
The specific activity of recombinant human IL-11 is approximately 1.05 x 104 units/μg, which is calibrated against recombinant human IL-11 WHO International Standard (NIBSC code: 92/788).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-11 protein
Pro22-Leu199
Accession #
N-terminal Sequence
Analysis
Pro22
Predicted Molecular Mass
19 kDa
SDS-PAGE
23 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

218-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

218-IL/CF

Formulation Supplied as a 0.2 μm filtered solution in PBS and EDTA.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Data Images

Bioactivity View Larger

Recombinant Human IL-11 (Catalog # 218‑IL) stimulates cell proliferation of the T11 mouse plasmacytoma cell line. The ED50for this effect is 0.02-0.12 ng/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Human IL-11 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: IL-11

IL-11 (Interleukin 11) is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27 and IL-31 (1-3). In humans, IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF) (3). The human IL-11 cDNA encodes a 199 amino acid (aa) precursor, which generates a 178 aa, 19 kDa mature unglycosylated protein. Mature human IL-11 shares 88%, 88%, and 96% aa sequence identity with mouse, rat and canine IL-11, respectively. IL-11 is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts, among other cell types (1). It is found in the plasma mainly during inflammation, such as that associated with viral infection, cancer, or inflammatory arthritis, and is considered to be primarily anti‑inflammatory (1). It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine (1). It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis (1). IL-11 synergizes with several other cytokines to produce these effects, and its effects overlap with those of IL-6 (1). IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11 R alpha subunit and two molecules of the ubiquitously expressed cell signaling beta subunit, gp130 (4). A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface, or inhibit cell surface IL-11 R alpha /gp130 signaling (5-7).

References
  1. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
  2. Paul, S.R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7512.
  3. Kawashima, I. et al. (1991) FEBS Lett. 283:199.
  4. Barton, V.A. et al. (2000) J. Biol. Chem. 275:36197.
  5. Curtis, D.J. et al. (1997) Blood 90:4403.
  6. Baumann, H. et al. (1996) J. Immunol. 157:284.
  7. Karow, J. et al. (1996) Biochem. J. 318:489.
Long Name
Interleukin 11
Entrez Gene IDs
3589 (Human); 16156 (Mouse); 171040 (Rat)
Alternate Names
Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11; Oprelvekin

Citations for Recombinant Human IL-11 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies
    Authors: B Mair, J Tomic, SN Masud, P Tonge, A Weiss, M Usaj, AHY Tong, JJ Kwan, KR Brown, E Titus, M Atkins, KSK Chan, L Munsie, A Habsid, H Han, M Kennedy, B Cohen, G Keller, J Moffat
    Cell Rep, 2019;27(2):599-615.e12.
    Species: Human
    Sample Types: cell culture
    Applications: Differentiation
  2. KDM3A histone demethylase functions as an essential factor for activation of JAK2-STAT3 signaling pathway
    Authors: H Kim, D Kim, SA Choi, CR Kim, SK Oh, KE Pyo, J Kim, SH Lee, JB Yoon, Y Zhang, SH Baek
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. BMTP-11 is active in preclinical models of human osteosarcoma and a candidate targeted drug for clinical translation
    Authors: VO Lewis, E Devarajan, M Cardó-Vila, DG Thomas, ES Kleinerman, S Marchiò, RL Sidman, R Pasqualini, W Arap
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes
    Immunology, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
    Authors: Gori J, Butler J, Chan Y, Chandrasekaran D, Poulos M, Ginsberg M, Nolan D, Elemento O, Wood B, Adair J, Rafii S, Kiem H
    J Clin Invest, 2015;125(3):1243-54.
    Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  6. MTA2 enhances colony formation and tumor growth of gastric cancer cells through IL-11.
    Authors: Zhou C, Ji J, Cai Q, Shi M, Chen X, Yu Y, Zhu Z, Zhang J
    BMC Cancer, 2015;15(0):343.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. A Novel Small-Molecule Inhibitor Targeting the IL-6 Receptor beta Subunit, Glycoprotein 130.
    Authors: Hong S, Choi J, Lee S, Park Y, Park K, Lee J, Kim J, Gajulapati V, Goo J, Singh S, Lee K, Kim Y, Im S, Ahn S, Rose-John S, Heo T, Choi Y
    J Immunol, 2015;195(1):237-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Expression of a surface antigen (MA6) by peripheral blood CD34+ cells is correlated with improved platelet engraftment and may explain delayed platelet engraftment following cord blood transplantation.
    Authors: Simmons P, Robinson S, Munsell M, Thomas M, Javni J, Brouard N, Zweidler-McKay P, Shpall E
    Stem Cells Dev, 2015;24(9):1066-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Regulation of IL-17A responses in human airway smooth muscle cells by Oncostatin M.
    Authors: Kwofie K, Scott M, Rodrigues R, Guerette J, Radford K, Nair P, Richards C
    Respir Res, 2015;16(0):14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis.
    Authors: Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M
    Blood, 2014;124(9):1393-403.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Autocrine production of IL-11 mediates tumorigenicity in hypoxic cancer cells.
    Authors: Onnis, Barbara, Fer, Nicole, Rapisarda, Annamari, Perez, Victor S, Melillo, Giovanni
    J Clin Invest, 2013;123(4):1615-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
    Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
    Int. J. Cancer, 2009;125(2):367-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression.
    Authors: Brechter AB, Lerner UH
    Arthritis Rheum., 2007;56(3):910-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells.
    Authors: Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, Zini R, Salati S, Tagliafico E, Manfredini R, Grande A, Ferrari S
    Cell Death Differ., 2006;13(10):1686-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K.
    Authors: Mandelin J, Li TF, Hukkanen M, Liljestrom M, Salo J, Santavirta S, Konttinen YT
    J. Rheumatol., 2005;32(4):713-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Combinations of cytokines promote survival of mice and limit acute radiation damage in concert with amelioration of vascular damage.
    Authors: Van der Meeren A, Mouthon MA, Vandamme M, Squiban C, Aigueperse J
    Radiat. Res., 2004;161(5):549-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004;173(5):3482-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Prospective isolation of human clonogenic common myeloid progenitors.
    Authors: Manz MG, Miyamoto T, Akashi K, Weissman IL
    Proc. Natl. Acad. Sci. U.S.A., 2002;99(18):11872-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Expression of interleukin (IL)-11 receptor by the human endometrium in vivo and effects of IL-11, IL-6 and LIF on the production of MMP and cytokines by human endometrial cells in vitro.
    Authors: Cork BA, Tuckerman EM, Li TC, Laird SM
    Mol. Hum. Reprod., 2002;8(9):841-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Interleukin-11 induces Th2 polarization of human CD4(+) T cells.
    Authors: Curti A, Ratta M, Corinti S, Girolomoni G, Ricci F, Tazzari P, Siena M, Grande A, Fogli M, Tura S, Lemoli RM
    Blood, 2001;97(9):2758-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human IL-11 Protein
By Anonymous on 05/26/2020
Application: Colloidal stability by SEC.
Reason for Rating: Some high molecular weight species observed by SEC.