Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF

Catalog # Availability Size / Price Qty
AVI10382-050
Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein Binding Activity
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Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 (Catalog # 219-IL) is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-12 beta 1 Fc Chimera Avi-tag (Catalog # AVI10382) that produces 50% of the optimal binding response is found to be approximately 15-90 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human IL-12 R beta 1 protein
Human IL-12 R beta 1
(Cys24-Glu540)
Accession # P42701-1
IEGRMDHuman IgG1
(Pro100-Lys330)
Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Cys24
Structure / Form
Disulfide-linked homodimer, biotinylated via Avi-tag
Predicted Molecular Mass
85 kDa
SDS-PAGE
95-115 kDa, under reducing conditions

Product Datasheets

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AVI10382

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10382

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein Binding Activity View Larger

When Recombinant Human IL-12 (Catalog # 219-IL) is immobilized at 1 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human IL-12 beta 1 Fc Chimera Avi-tag (Catalog # AVI10382) that produces 50% of the optimal binding response if found to be approximately 15-90 ng/mL.

SDS-PAGE Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human IL-12 R beta 1 Fc Chimera Avi-tag (Catalog # AVI10382) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-115 kDa and 180-230 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

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Background: IL-12 R beta 1

IL12RB1 (Interleukin-12 receptor subunit beta-1; IL‑12 R beta 1), also known as CD212, is a type I transmembrane protein with similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL12RB1, along with IL12RB2, are two receptor subunits for IL12. Individually, the two subunits bind IL12 with low affinity, but when combined they form a high-affinity receptor complex (1). Human IL12RB1 contains 5 fibronectin domains in the extracellular domain (ECD), a single transmembrane domain and a box 1 motif in the cytoplasmic region. The mature ECD of human IL12RB1 shares 51% amino acid identity with mouse and rat IL12RB1. Interleukin 12 (IL12) is a pro-inflammatory cytokine and key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells (2). Binding of IL12RB1, along with IL12RB2, is required for IL12 signal transduction and subsequent activity (1,2). Expression of IL12RB1 been detected in activated T cells, NK cells and B cells (1). The expression of IL12RB2 is more restricted and appears to be limited to Th2 cells. IL12RB1 has been reported to interact with IL23R to form the functional receptor complex for IL23 and probably activation of the Jak-Stat signaling cascade (3).

References
  1. Robinson R.T. (2015) Cytokine. 71:348.
  2. Rosenzweig S.D. and Holland S.M. (2005) Immunol. Rev. 203:38.
  3. Zou J. et al. (1997) J. Biol Chem, 272:6073.
Long Name
Interleukin 12 Receptor beta 1
Entrez Gene IDs
3594 (Human); 16161 (Mouse)
Alternate Names
CD212 antigen; CD212; IL-12 R beta 1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454

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