Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF Summary
|Human IL-12 R beta 1|
Accession # P42701-1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human IL-12 (Catalog # 219-IL) is immobilized at 1 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human IL-12 beta 1 Fc Chimera Avi-tag (Catalog # AVI10382) that produces 50% of the optimal binding response if found to be approximately 15-90 ng/mL.
2 μg/lane of Biotinylated Recombinant Human IL-12 R beta 1 Fc Chimera Avi-tag (Catalog # AVI10382) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-115 kDa and 180-230 kDa, respectively.
Background: IL-12 R beta 1
IL12RB1 (Interleukin-12 receptor subunit beta-1; IL‑12 R beta 1), also known as CD212, is a type I transmembrane protein with similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL12RB1, along with IL12RB2, are two receptor subunits for IL12. Individually, the two subunits bind IL12 with low affinity, but when combined they form a high-affinity receptor complex (1). Human IL12RB1 contains 5 fibronectin domains in the extracellular domain (ECD), a single transmembrane domain and a box 1 motif in the cytoplasmic region. The mature ECD of human IL12RB1 shares 51% amino acid identity with mouse and rat IL12RB1. Interleukin 12 (IL12) is a pro-inflammatory cytokine and key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells (2). Binding of IL12RB1, along with IL12RB2, is required for IL12 signal transduction and subsequent activity (1,2). Expression of IL12RB1 been detected in activated T cells, NK cells and B cells (1). The expression of IL12RB2 is more restricted and appears to be limited to Th2 cells. IL12RB1 has been reported to interact with IL23R to form the functional receptor complex for IL23 and probably activation of the Jak-Stat signaling cascade (3).
- Robinson R.T. (2015) Cytokine. 71:348.
- Rosenzweig S.D. and Holland S.M. (2005) Immunol. Rev. 203:38.
- Zou J. et al. (1997) J. Biol Chem, 272:6073.
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