Recombinant Human IL-17A/F Heterodimer Biotinylated Protein

  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4‑2 ng/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
     Human IL-17A
    (Gly24-Ala155)
    Accession # Q16552
    Human IL-17F 
    (Arg21-GLN163)
    Accession # Q96PD4
    N-terminusC-terminus
  • N-terminal Sequence
    Analysis
    Gly24(IL-17A) & Arg21(IL-17F)
  • Structure / Form
    Disulfide-linked heterodimer, Biotinylated via sugars
  • Predicted Molecular Mass
    15 kDa (unlabeled IL-17A), 15 kDa (unlabeled IL-17F)
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
BT5837
 
BT5837/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Both Biotinylated Recombinant Human IL-17A/F (Catalog # BT5837) and unlabeled Recombinant Human IL‑17A/F Heterodimer (Catalog #
5837-IL) induces IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-2 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional.
Background: IL-17A/F Heterodimer
Human IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (3-5). Mature human IL-17A and IL-17F share 61% and 56% amino acid sequence identity with mouse IL-17A and IL-17F, respectively. They share 50% aa sequence identity with each other. IL-17A/F and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).
  • References:
    1. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
    2. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
    3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
    4. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
    5. Gu, C. et al. (2013) Cytokine 64:477.
    6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
    7. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
    8. Ouyang, W. et al. (2008) Immunity 28:454.
    9. Steinman, L. (2007) Nat. Med. 13:139.
    10. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
    11. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
    12. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
    13. Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.
  • Long Name:
    Interleukin 17 A/F
  • Alternate Names:
    CTLA8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; Cytotoxic T-Lymphocyte-Associated Protein 8; IL17; IL-17; IL-17A; IL-17A/F Heterodimer; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; interleukin-17A
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