Recombinant Human IL-17A/F Heterodimer (CHO-expressed), CF Summary
Accession # Q16552
Accession # Q96PD4
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in HCl.|
|Reconstitution||Reconstitute at 100 μg/mL in 4 mM HCI.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: IL-17A/F Heterodimer
Human IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (3-5). Mature human IL-17A and IL-17F share 61% and 56% amino acid sequence identity with mouse IL-17A and IL-17F, respectively. They share 50% aa sequence identity with each other. IL-17A/F and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).
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- Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
- Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
- Kolls, J.K. and A. Linden (2004) Immunity 21:467.
- Gu, C. et al. (2013) Cytokine 64:477.
- Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
- Liang, S.C. et al. (2007) J. Immunol. 179:7791.
- Ouyang, W. et al. (2008) Immunity 28:454.
- Steinman, L. (2007) Nat. Med. 13:139.
- Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
- Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
- Wright, J.F. et al. (2008) J. Immunol. 181:2799.
- Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.
Citation for Recombinant Human IL-17A/F Heterodimer (CHO-expressed), CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S
Eur J Immunol, 2015;46(2):440-5.
Sample Types: Whole Cells
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