Recombinant Human IL-17E (Human Cell-expressed) Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
8134-IL-025/CF
Recombinant Human IL-17E (Human Cell-expressed) Protein, CF Bioactivity
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Recombinant Human IL-17E (Human Cell-expressed) Protein, CF Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IL-17E/IL-25 protein
Tyr33-Gly177
Accession #
N-terminal Sequence
Analysis
Tyr33
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
17 kDa (monomer)
SDS-PAGE
20-28 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8134-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8134-IL

Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human IL-17E (Human Cell-expressed) Protein, CF Bioactivity View Larger

Recombinant Human IL-17E (Catalog # 8134-IL/CF) induces CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.

SDS-PAGE Recombinant Human IL-17E (Human Cell-expressed) Protein, CF SDS-PAGE View Larger

1 μg/lane ofRecombinant Human IL-17E (Catalog # 8134-IL/CF) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by silverstaining, showing R bands at
20-28 kDa and NR bands at 37-43 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17E/IL-25

The Interleukin-17 (IL-17) family of proteins are immunoregulatory cytokines that share a conserved cysteine-rich region. IL-17E, which is also known as IL-25, promotes Th2-biased immune responses. This is in contrast to other IL-17 family members which promote Th1- and Th17-biased inflammation. IL-25 is an important mediator of allergic reactions and protection against intestinal parasites (1, 2). Mature human IL-25 shares 80% amino acid sequence identity with mouse and rat IL-25 (3, 4). During helminth infections and allergic reactions, IL-25 is locally up-regulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils (4-9). It binds to IL-17 RB but also requires IL-17 RA to exert its activity (3, 8, 10). IL-25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL-4, IL-5, IL-13) and reduced production of Th1 and Th17 cytokines (e.g. IFN-gamma, IL-12, IL-23, IL-17A, IL-17F) (4-6, 8, 9, 11-15). Airway IL-25 can be activated by MMP-7, a protease that is up-regulated in airway epithelium in response to allergen exposure (16). Cleaved IL-25 shows enhanced binding to IL-17 RB and stronger induction of Th2 cytokines (16). The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and
anti‑inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration (4, 6, 9, 13). These actions promote protective
anti‑helminth immune responses (4, 5) as well as allergic inflammation and airway hyperreactivity (11). The IL-25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis [12, 15]. IL-25 also promotes vascular endothelial cell proliferation and assembly into tubular structures (7). It supports the integrity of the blood-brain barrier and limits CD4+ T cell infiltration into the brain (17).

References
  1. Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
  2. Iwakura, Y. et al. (2011) Immunity 34:149.
  3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
  4. Fort, M.M. et al. (2001) Immunity 15:985.
  5. Zhao, A. et al. (2010) J. Immunol. 185:6921.
  6. Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
  7. Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
  8. Petersen, B.C. et al. (2012) Nat. Med. 18:751.
  9. Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
  10. Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
  11. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
  12. Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161.
  13. Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229.
  14. Stock, P. et al. (2009) J. Immunol. 182:5116.
  15. Caruso, R. et al. (2009) Gastroenterology 136:2270.
  16. Goswami, S. et al. (2009) Nat. Immunol. 10:496.
  17. Sonobe, Y. et al. (2009) J. Biol. Chem. 284:31834.
Long Name
Interleukin 17E
Entrez Gene IDs
64806 (Human); 140806 (Mouse); 501996 (Rat)
Alternate Names
IL17E; IL-17E; IL17Einterleukin-17E; IL25; IL-25; interleukin 17E; interleukin 25; Interleukin-17E; interleukin-25

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