Recombinant Human IL-17E (Human Cell-expressed) Protein, CF

  
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Tyr33-Gly177
  • Accession #
  • N-terminal Sequence
    Analysis
    Tyr33
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    17 kDa (monomer)
  • SDS-PAGE
    20-28 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8134-IL/CF
 
8134-IL
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
IL-17E Induces CXCL1/GRO alpha Secretion.
Recombinant Human IL-17E (Catalog # 8134-IL/CF) induces CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.
1 μg/lane ofRecombinant Human IL-17E (Catalog # 8134-IL/CF) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by silverstaining, showing R bands at
20-28 kDa and NR bands at 37-43 kDa.
Background: IL-17E/IL-25
The Interleukin-17 (IL-17) family of proteins are immunoregulatory cytokines that share a conserved cysteine-rich region. IL-17E, which is also known as IL-25, promotes Th2-biased immune responses. This is in contrast to other IL-17 family members which promote Th1- and Th17-biased inflammation. IL-25 is an important mediator of allergic reactions and protection against intestinal parasites (1, 2). Mature human IL-25 shares 80% amino acid sequence identity with mouse and rat IL-25 (3, 4). During helminth infections and allergic reactions, IL-25 is locally up-regulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils (4-9). It binds to IL-17 RB but also requires IL-17 RA to exert its activity (3, 8, 10). IL-25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL-4, IL-5, IL-13) and reduced production of Th1 and Th17 cytokines (e.g. IFN-gamma, IL-12, IL-23, IL-17A, IL-17F) (4-6, 8, 9, 11-15). Airway IL-25 can be activated by MMP-7, a protease that is up-regulated in airway epithelium in response to allergen exposure (16). Cleaved IL-25 shows enhanced binding to IL-17 RB and stronger induction of Th2 cytokines (16). The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and
anti‑inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration (4, 6, 9, 13). These actions promote protective
anti‑helminth immune responses (4, 5) as well as allergic inflammation and airway hyperreactivity (11). The IL-25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis [12, 15]. IL-25 also promotes vascular endothelial cell proliferation and assembly into tubular structures (7). It supports the integrity of the blood-brain barrier and limits CD4+ T cell infiltration into the brain (17).
  • References:
    1. Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
    2. Iwakura, Y. et al. (2011) Immunity 34:149.
    3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
    4. Fort, M.M. et al. (2001) Immunity 15:985.
    5. Zhao, A. et al. (2010) J. Immunol. 185:6921.
    6. Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
    7. Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
    8. Petersen, B.C. et al. (2012) Nat. Med. 18:751.
    9. Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
    10. Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
    11. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
    12. Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161.
    13. Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229.
    14. Stock, P. et al. (2009) J. Immunol. 182:5116.
    15. Caruso, R. et al. (2009) Gastroenterology 136:2270.
    16. Goswami, S. et al. (2009) Nat. Immunol. 10:496.
    17. Sonobe, Y. et al. (2009) J. Biol. Chem. 284:31834.
  • Long Name:
    Interleukin 17E
  • Entrez Gene IDs:
    64806 (Human); 140806 (Mouse); 501996 (Rat)
  • Alternate Names:
    IL17E; IL-17E; IL17Einterleukin-17E; IL25; IL-25; interleukin 17E; interleukin 25; Interleukin-17E; interleukin-25
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