Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. Human IL‑17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy‑proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL‑17 includes activation of NF‑ kappa B and JNK, and require TNF receptor‑associated factors 6 (TRAF6) in the signaling pathway.
Recombinant Human IL-17RA/IL-17R His-tag Protein, CF
R&D Systems | Catalog # 11234-IR
Analyzed by SEC-MALS
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Key Product Details
- R&D Systems CHO-derived Recombinant Human IL-17RA/IL-17R His-tag Protein (11234-IR)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
CHO
Accession Number
Applications
Bioactivity
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Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human IL-17RA/IL-17R protein
Leu33-Trp320, with a C-terminal 6-His tag.
Leu33-Trp320, with a C-terminal 6-His tag.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu33
Predicted Molecular Mass
34 kDa
SDS-PAGE
60-68 kDa, under reducing conditions.
Activity
Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.050‑1.00 µg/mL in the presence of 10.0 ng/mL recombinant human IL‑17
(Catalog #
7955-IL).
Scientific Data Images for Recombinant Human IL-17RA/IL-17R His-tag Protein, CF
Recombinant Human IL-17RA/IL-17R His-tag Protein SEC-MALS.
Recombinant Human IL-17RA/IL-17R His-tag Protein (Catalog # 11234-IR) has a molecular weight (MW) of 50.4 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).
Recombinant Human IL-17RA/IL-17R His-tag Protein Bioactivity.
Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.050‑1.00 µg/mL in the presence of 10.0 ng/mL Recombinant Human IL‑17A (7955-IL).
Recombinant Human IL-17RA/IL-17R His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human IL-17RA/IL-17R His-tag Protein (Catalog # 11234-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-68 kDa.Formulation, Preparation, and Storage
11234-IR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: IL-17RA/IL-17R
References
- Yao, Z. et al. (1997) Cytokine 9:794.
- Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
Long Name
Interleukin 17 Receptor
Alternate Names
CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA
Entrez Gene IDs
Gene Symbol
IL17RA
UniProt
Additional IL-17RA/IL-17R Products
Product Documents for Recombinant Human IL-17RA/IL-17R His-tag Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human IL-17RA/IL-17R His-tag Protein, CF
For research use only
Related Research Areas
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