Recombinant Human IL-18 R alpha/IL-1 R5 Avi His Protein, CF

Catalog #: AVI11179 Datasheet / COA / SDS

Biotinylated

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AVI11179-050

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Recombinant Human IL-18 R alpha/IL-1 R5 Avi-tag His-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-18 R alpha/IL-1 R5 Avi His Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-18 R beta/IL-1 R7 Fc (Catalog # 118-AP) is immobilized at 0.500 µg/mL (100 µL/well), in the present of Recombinant Human IL-18 (Catalog # 9124-IL) Biotinylated Recombinant Human IL‑18 R alpha /IL‑1 R5 Avi-tag His-tag (Catalog # AVI11179) binds with an ED₅₀ of 20.0-200 ng/mL.

The biotin to protein ratio is great than 0.7 as determined by the HABA assay.

Source

Human embryonic kidney cell, HEK293-derived human IL-18 R alpha/IL-1 R5 protein

Human IL-18 R alpha /IL-1 R5 (Cys22-Arg329) Accession # Q13478.1	Avi-tag	6-His tag
N-terminus		C-terminus

Accession #

Q13478.1

N-terminal Sequence
Analysis

Cys22

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

40 kDa

SDS-PAGE

63-71 kDa, under reducing conditions.

Product Datasheets

AVI11179

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11179

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. When Recombinant Human IL-18 R beta/IL-1 R7 Fc (118-AP) is immobilized at 0.500 µg/mL (100 µL/well), in the present of Recombinant Human IL-18/IL-1F4 (9124-IL), Biotinylated Recombinant Human IL‑18 R alpha/IL‑1 R5 Avi-tag His-tag (Catalog # AVI11179) binds with an ED₅₀ of 20.0-200 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human IL-18 R alpha/IL-1 R5 Avi-tag His-tag Protein (Catalog # AVI11179) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 63-71 kDa.

Reconstitution Calculator

Background:

IL-18 Receptor alpha (IL-18 Ra), also known as IL-18 R1, IL-1 R5, and IL-1 Rrp, is a transmembrane component of the IL-18 Receptor (1). It associates with IL‑18 Rb/IL‑1 R7 to form the high affinity receptor complex for IL-18 and confer cellular responsiveness to IL-18 (2, 3). IL-18 is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. Mature human IL-18 Ra consists of a 308 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 191 aa cytoplasmic domain (4). Within the ECD, human IL-18 Ra shares 56% aa sequence identity with mouse and rat IL-18 Ra. IL-18 Ra is widely expressed on epithelial cells as well as on B cells, NK cells, CD4+ and CD8+ T cells, and dendritic cells (5-8). Signaling through the IL-18 receptor promotes a Th1 biased immune response and NK cell cytolytic activity (6, 7, 9-11). It also contributes to cigarette smoke-induced airway inflammation and epithelial damage (12). Our Avi-tag Biotinylated human IL-18 Ra features biotinylation at a single site contained within the avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound the streptavidin-coated surface due to the precise control of biotinylatin and the rest of the protein in changed so there is no interference in the protein's bioactivity.

References