Recombinant Human IL-21 R His-tag Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit IL-21-dependent proliferation of N1186 human T cells Parrish-Novak, J. et al. (2000) Nature 408:57. The ED50 for this effect is typically 0.6-3.6 µg/mL in the presence of 100 ng/mL of recombinant human IL-21.
  • Source
    Human embryonic kidney cell, HEK293-derived Cys20-Pro236, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Cys20
  • Predicted Molecular Mass
    26 kDa
  • SDS-PAGE
    45-53 kDa, reducing conditions
9249-R2
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
RecombinantHuman IL-21 R (Catalog # 9249-R2) inhibits IL-21-dependent proliferation ofN1186 human T cells. The ED50 for this effect is
0.6-3.6 μg/mL.
Background: IL-21 R
Interleukin-21 Receptor (IL-21 R )is a type I transmembrane glycoprotein within the class I cytokine receptor family (1). IL-21 R associates with the common gamma  chain ( gamma c) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15 (2, 3). Mature human IL-21 R consists of a 213 amino acid (aa) extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 285 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). Within the ECD, human IL-21 R shares 69% aa identity with mouse and rat IL-21 R, respectively. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 4, 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (6). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses (7, 8). IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production (4, 9), control of viral infection and tumor growth by CD8+ T cells (10), development of regulatory T cells (11), IL-23 responsiveness of encephalitogenic Th17 cells (12), but suppresses the accumulation of IL-17 secreting gamma δ T cells in the airway (13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (14, 15).
  • References:
    1. Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107.
    2. Asao, H. et al. (2001) J. Immunol. 167:1.
    3. Habib, T. et al. (2002) Biochemistry 41:8725.
    4. Parrish-Novak, et al. (2000) Nature 408:57.
    5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
    6. Ozaki, K. et al. (2002) Science 298:1630.
    7. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
    8. King, I.L. et al. (2010) J. Immunol. 185:6138.
    9. Kasaian, M.T. et al. (2002) Immunity 16:559.
    10. Frohlich, A. et al. (2009 Science 324:1576.
    11. Tortola, L. et al. (2010) Blood 116:5200.
    12. Lee, Y. et al. (2015) J. Clin. Invest. 125:4011.
    13. Moser, E.K. et al. (2015) PLoS One 10:e0120169.
    14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
    15. Sarosiek, K.A. et al. (2010) Blood 115:570.
  • Long Name:
    Interleukin 21 Receptor
  • Entrez Gene IDs:
    50615 (Human); 60504 (Mouse)
  • Alternate Names:
    CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor
Related Research Areas
Primary Antibodies
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His Tag Alexa Fluor® 488-conjugated Antibody

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His Tag Alexa Fluor® 700-conjugated Antibody

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His Tag PerCP-conjugated Antibody

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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

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