Recombinant Human IL-33 Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
3625-IL-010/CF
Recombinant Human IL-33 Protein, CF Bioactivity
2 Images
Product Details
Citations (32)
FAQs
Reviews

Recombinant Human IL-33 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.06-0.24 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
E. coli-derived human IL-33 protein
Ser112-Thr270
Accession #
N-terminal Sequence
Analysis
Ser112
Predicted Molecular Mass
18 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3625-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3625-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human IL-33 Protein, CF Bioactivity View Larger

Recombinant Human IL-33 (Catalog #
3625‑IL/CF) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 0.06-0.24 ng/mL.

SDS-PAGE Recombinant Human IL-33 Protein, CF SDS-PAGE View Larger

1 μg/lane of Recombinant Human IL-33 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF‑HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL‑33 was identified based on sequence and structural homology with IL‑1 family cytokines (3). DVS 27, NF‑HEV, and IL‑33 share 100% amino acid sequence identity. IL‑33 is constitutively expressed in smooth muscle and airway epithelia. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL‑1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL‑1, IL‑33 can be cleaved in vitro by caspase‑1, generating an N‑terminal fragment that is slightly shorter than the C‑terminal fragment (3, 4). The N‑terminal portion of full length IL‑33 contains a predicted bipartite nuclear localization sequence and a homeodomain‑like helix‑turn‑helix DNA binding domain. By immunofluorescence, full length IL‑33 localizes to the nucleus in HUVECs and transfectants (2). The C‑terminal fragment, corresponding to mature IL‑33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5‑7). A ternary signaling complex is formed by the subsequent association of IL‑33 and ST2L with IL‑1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL‑33 in vitro induces IL‑5 and IL‑13 secretion (3). In vivo administration of mature IL‑33 promotes increased production of IL‑5, IL‑13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature human IL‑33 share
52‑58% aa sequence identity with mouse and rat IL‑33. Human IL‑33 shares less than 20% aa sequence identity with other IL‑1 family proteins.

References
  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Long Name
Interleukin 33
Entrez Gene IDs
90865 (Human); 77125 (Mouse)
Alternate Names
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

Citations for Recombinant Human IL-33 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. IL-33 Initiates Vascular Remodelling in Hypoxic Pulmonary Hypertension by up-Regulating HIF-1? and VEGF Expression in Vascular Endothelial Cells
    Authors: J Liu, W Wang, L Wang, S Chen, B Tian, K Huang, CJ Corrigan, S Ying, W Wang, C Wang
    EBioMedicine, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Soluble ST2 suppresses IL-5 production by human basophilic KU812 cells, induced by epithelial cell-derived IL-33
    Authors: K Matsumoto, H Kouzaki, H Kikuoka, T Kato, I Tojima, S Shimizu, T Shimizu
    Allergol Int, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Seasonal variation in circulating group 2 innate lymphoid cells in mugwort-allergic asthmatics during and outside pollen season
    Authors: Q Miao, Y Wang, YG Liu, YX Ren, H Guan, Z Li, W Xu, L Xiang
    Allergy Asthma Clin Immunol, 2018;14(0):6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. IL-33 promotes colon cancer cell stemness via JNK activation and macrophage recruitment
    Authors: M Fang, Y Li, K Huang, S Qi, J Zhang, W Zgodzinski, M Majewski, G Wallner, S Gozdz, P Macek, A Kowalik, M Pasiarski, E Grywalska, L Vatan, N Nagarsheth, W Li, L Zhao, I Kryczek, G Wang, Z Wang, W Zou, L Wang
    Cancer Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. IL?33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin ?4?1 and CD62L
    Authors: XH Wang, W Liu, DX Fan, WT Hu, MQ Li, XY Zhu, LP Jin
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Interleukin-33 modulates inflammation in endometriosis
    Authors: JE Miller, SP Monsanto, SH Ahn, K Khalaj, AT Fazleabas, SL Young, BA Lessey, M Koti, C Tayade
    Sci Rep, 2017;7(1):17903.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. SP and IL-33 together markedly enhance TNF synthesis and secretion from human mast cells mediated by the interaction of their receptors
    Authors: A Taracanova, M Alevizos, A Karagkouni, Z Weng, E Norwitz, P Conti, SE Leeman, TC Theoharide
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
  8. Effector and regulatory dendritic cells display distinct patterns of miRNA expression
    Authors: V Lombardi, S Luce, H Moussu, L Morizur, C Gueguen, C Neukirch, S Chollet-Ma, L Mascarell, M Aubier, V Baron-Bodo, P Moingeon
    Immun Inflamm Dis, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. IL-33 blockade suppresses tumor growth of human lung cancer through direct and indirect pathways in a preclinical model
    Authors: K Wang, S Shan, Z Yang, X Gu, Y Wang, C Wang, T Ren
    Oncotarget, 2017;8(40):68571-68582.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis
    Authors: D Díaz-Jimén, L Núñez, M De la Fuen, K Dubois-Cam, H Sepúlveda, M Montecino, A Torres-Riq, P García-Gon, J Chnaiderma, A Vossenkamp, TT MacDonald, D Simian, MJ González, JA Cidlowski, R Quera, MA Hermoso
    Sci Rep, 2017;7(1):10180.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. IL?33 enhances glioma cell migration and invasion by upregulation of MMP2 and MMP9 via the ST2-NF-?B pathway
    Authors: JF Zhang, P Wang, YJ Yan, Y Li, MW Guan, JJ Yu, XD Wang
    Oncol. Rep., 2017;38(4):2033-2042.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes
    Immunology, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs
    Authors: SM Bal, JH Bernink, M Nagasawa, J Groot, MM Shikhagaie, K Golebski, CM van Drunen, R Lutter, RE Jonkers, P Hombrink, M Bruchard, J Villaudy, JM Munneke, W Fokkens, JS Erjefält, H Spits, XR Ros
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation
    Sci Rep, 2016;6(0):25171.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Soluble IL-33 receptor sST2 inhibits colorectal cancer malignant growth by modifying the tumour microenvironment
    Nat Commun, 2016;7(0):13589.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells
    Sci Rep, 2016;6(0):36795.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. IL-33 activates tumor stroma to promote intestinal polyposis.
    Authors: Maywald R, Doerner S, Pastorelli L, De Salvo C, Benton S, Dawson E, Lanza D, Berger N, Markowitz S, Lenz H, Nadeau J, Pizarro T, Heaney J
    Proc Natl Acad Sci U S A, 2015;112(19):E2487-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Interleukin-33/ST2 axis promotes epithelial cell transformation and breast tumorigenesis via upregulation of COT activity.
    Authors: Kim J, Lim S, Kim G, Yun H, Ahn S, Choi H
    Oncogene, 2015;34(38):4928-38.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  19. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
    Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
    J Transl Med, 2015;13(0):381.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins alpha-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.
    Authors: Kempuraj D, Thangavel R, Yang E, Pattani S, Zaheer S, Santillan D, Santillan M, Zaheer A
    PLoS ONE, 2015;10(8):e0135776.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. BCR-ABL-induced deregulation of the IL-33/ST2 pathway in CD34+ progenitors from chronic myeloid leukemia patients.
    Authors: Levescot A, Flamant S, Basbous S, Jacomet F, Feraud O, Anne Bourgeois E, Bonnet M, Giraud C, Roy L, Barra A, Chomel J, Turhan A, Guilhot F, Girard J, Gombert J, Herbelin A
    Cancer Res, 2014;74(10):2669-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Eosinophils regulate peripheral B cell numbers in both mice and humans.
    Authors: Wong, Tina W, Doyle, Alfred D, Lee, James J, Jelinek, Diane F
    J Immunol, 2014;192(8):3548-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Interleukin-33 induces urokinase in human endothelial cells--possible impact on angiogenesis.
    Authors: Stojkovic S, Kaun C, Heinz M, Krychtiuk K, Rauscher S, Lemberger C, de Martin R, Groger M, Petzelbauer P, Huk I, Huber K, Wojta J, Demyanets S
    J Thromb Haemost, 2014;12(6):948-57.
  24. The alarmin concept applied to human renal transplantation: evidence for a differential implication of HMGB1 and IL-33.
    Authors: Thierry A, Giraud S, Robin A, Barra A, Bridoux F, Ameteau V, Hauet T, Girard J, Touchard G, Gombert J, Herbelin A
    PLoS ONE, 2014;9(2):e88742.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
    Authors: Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J
    J Invest Dermatol, 2014;134(9):2351-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Anti-inflammatory and anti-allergic activities of Pentaherb formula, Moutan Cortex (Danpi) and gallic acid.
    Authors: Liu K, Hu S, Chan B, Wat E, Lau C, Hon K, Fung K, Leung P, Hui P, Lam C, Wong C
    Molecules, 2013;18(3):2483-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  27. Muramyl dipeptide mediated activation of human bronchial epithelial cells interacting with basophils: a novel mechanism of airway inflammation.
    Authors: Qiu H, Wong C, Chu I, Hu S, Lam C
    Clin Exp Immunol, 2013;172(1):81-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33.
    J. Exp. Med., 2012;209(8):1505-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Serum neurotensin (NT) is increased in psoriasis and NT induces vascular endothelial growth factor release from human mast cells.
    Authors: Vasiadi M, Therianou A, Alysandratos KD, Katsarou-Katsari A, Petrakopoulou T, Theoharides A, Papadavid E, Stavrianeas N, Antoniou C, Kalogeromitros D, Theoharides TC
    Br. J. Dermatol., 2012;166(6):1349-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Daily exposure to dust alters innate immunity.
    Authors: Sahlander K, Larsson K, Palmberg L
    PLoS ONE, 2012;7(2):e31646.
    Species: Human
    Sample Types: Whole Blood
    Applications: Bioassay
  31. A novel IL-1 family cytokine, IL-33, potently activates human eosinophils.
    Authors: Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H
    J. Allergy Clin. Immunol., 2008;121(6):1484-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
    Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
    J Korean Med Sci, 0;26(9):1132-9.

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human IL-33 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human IL-33 Protein, CF and earn rewards!

Have you used Recombinant Human IL-33 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review