Recombinant Human IL-3R alpha/CD123 Protein

Name: Recombinant Human IL-3R alpha/CD123 Protein
Brand: R&D Systems
SKU: 301-R3
Price: 592 USD
Availability: InStock

R&D Systems | Catalog # 301-R3

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Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems NS0-derived Recombinant Human IL-3R alpha/CD123 Protein (301-R3)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P26951

Applications

Binding Activity

View all IL-3R alpha Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IL-3 R alpha/CD123 protein
Lys20-Arg305

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys20

Predicted Molecular Mass

33 kDa

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL‑3 R alpha /CD123 is present at 1 μg/mL, the concentration of Recombinant Human IL‑3 (Catalog # 203-IL) that produces 50% of the optimal binding response is found to be approximately 8‑40 ng/mL.

Scientific Data Images for Recombinant Human IL-3R alpha/CD123 Protein

Recombinant Human IL-3R alpha/CD123 Protein Binding Activity

Recombinant Human IL-3 Ra (Catalog # 301-R3) binds Recombinant Human IL-3 in a functional ELISA. When Recombinant Human IL-3 Ra is present at 1 µg/mL, the concentration of Recombinant Human IL-3 (Catalog # 203-IL) that produces 50% of the optimal binding response is approximately 8-40 ng/mL.

Recombinant Human IL-3R alpha/CD123 Protein SDS-PAGE

1 μg/lane of Recombinant Human IL-3 R alpha / CD123 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 55 kDa and 58 kDa.

Formulation, Preparation, and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 301-R3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Carrier Free: 301-R3/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-3R alpha

Interleukin 3 is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL‑3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage‑committed cells. The biological effects of IL‑3 on the various cell types are mediated by the binding of IL‑3 to specific cell surface receptor complexes. The functional high‑affinity human IL‑3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL‑3 with low affinity. The beta subunit does not bind IL‑3 by itself but is required for the high‑affinity binding of IL‑3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high‑affinity receptor complex for IL‑5 and GM‑CSF. Both the alpha and the beta subunits are members of the cytokine receptor superfamily.