Recombinant Human IL-3R alpha/CD123 Protein

Carrier Free

Catalog # Availability Size / Price Qty
301-R3-025/CF

With Carrier

Catalog # Availability Size / Price Qty
301-R3-025
Recombinant Human IL-3R alpha/CD123 Protein Binding Activity
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Product Details
Citations (2)
FAQs
Reviews

Recombinant Human IL-3R alpha/CD123 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑3 R alpha /CD123 is present at 1 μg/mL, the concentration of Recombinant Human IL‑3 (Catalog # 203-IL) that produces 50% of the optimal binding response is found to be approximately 8‑40 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-3 R alpha/CD123 protein
Lys20-Arg305
Accession #
N-terminal Sequence
Analysis
Lys20
Predicted Molecular Mass
33 kDa

Product Datasheets

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301-R3 (with carrier)

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301-R3/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

301-R3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

301-R3/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human IL-3R alpha/CD123 Protein Binding Activity View Larger

Recombinant Human IL-3 Ra (Catalog # 301-R3) binds Recombinant Human IL-3 in a functional ELISA. When Recombinant Human IL-3 Ra is present at 1 µg/mL, the concentration of Recombinant Human IL-3 (Catalog # 203-IL) that produces 50% of the optimal binding response is approximately 8-40 ng/mL.

SDS-PAGE Recombinant Human IL-3R alpha/CD123 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human IL-3 R alpha / CD123 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 55 kDa and 58 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-3R alpha

Interleukin 3 is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL‑3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage‑committed cells. The biological effects of IL‑3 on the various cell types are mediated by the binding of IL‑3 to specific cell surface receptor complexes. The functional high‑affinity human IL‑3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL‑3 with low affinity. The beta  subunit does not bind IL‑3 by itself but is required for the high‑affinity binding of IL‑3 to the heterodimeric receptor complex. The beta  subunit has also been found to be a component of the high‑affinity receptor complex for IL‑5 and GM‑CSF. Both the alpha and the beta  subunits are members of the cytokine receptor superfamily.

References
  1. Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.
Long Name
Interleukin 3 Receptor alpha
Entrez Gene IDs
3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)
Alternate Names
CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174

Citations for Recombinant Human IL-3R alpha/CD123 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CD123-Engager T Cells as a Novel Immunotherapeutic for Acute Myeloid Leukemia
    Mol Ther, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: CAR-T (ELISA capture)
  2. CD123 targeting oncolytic adenoviruses suppress acute myeloid leukemia cell proliferation in vitro and in vivo.
    Authors: Li, G, Li, X, Wu, H, Yang, X, Zhang, Y, Chen, L, Wu, X, Cui, L, Wu, L, Luo, J, Liu, X Y
    Blood Cancer J, 2014;4(0):e194.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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