Recombinant Human IL-4R alpha Protein

Newer Version Available: 230-4RB
A New and Improved rh IL-4 Ra is Now Available! It has ~2 fold better activity!
Formulations:
Catalog # Availability Size / Price Qty
230-4R-025
Product Details
Citations (3)
FAQs
Reviews

Recombinant Human IL-4R alpha Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. Approximately 5-25 ng/mL of IL-4 R alpha will inhibit 50% of the biological response due to 0.2 ng/mL of recombinant human IL-4.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-4 R alpha protein
Gly24-His232
Accession #
N-terminal Sequence
Analysis
Gly24
Predicted Molecular Mass
24 kDa
SDS-PAGE
30-35 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

230-4R

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

230-4R/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-4R alpha

Interleukin 4 Receptor alpha (IL-4 R alpha ), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL-4 R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1 - 5). Mature human IL-4 R alpha consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type-III domain, a 24 aa transmembrane segment, and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6, 7). Within the ECD, human IL-4 Ra shares 51% aa sequence identity with mouse and rat IL-4 R alpha. Soluble forms of IL-4 R alpha, generated by alternate splicing or proteolysis, retain ligand binding properties and inhibit IL-4 bioactivity (8 - 11). IL-4 R alpha is a component of two distinct receptor complexes and shows species selectivity between human and mouse (6). It can associate with the common gamma chain ( gamma c) to form the IL-4 responsive type I receptor in which gamma c increases the affinity for IL-4 and enables signaling (12, 13). It can alternatively associate with IL-13 R alpha 1 to form the type II receptor which is responsive to both IL-4 and IL-13 (14, 15). The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL‑13 as well as other cytokines that utilize gamma c (i.e. IL-2, IL-7, IL-9, IL-15, and IL-21) (16, 17).

References
  1. Wills-Karp, M. and F.D. Finkelman (2008) Sci. Signal. 1:pe55.
  2. Gordon, S. and F.O. Martinez (2010) Immunity 32:593.
  3. Kuperman, D.A. and R.P. Schleimer (2008) Curr. Mol. Med. 8:384.
  4. Li, Z. et al. (2009) Cell. Mol. Immunol. 6:415.
  5. Lee, Y.W. et al. (2010) Biomol. Ther. 18:135.
  6. Idzerda, R.L. et al. (1990) J. Exp. Med. 171:861.
  7. Galizzi, J.P. et al. (1990) Int. Immunol. 2:669.
  8. Kruse, S. et al. (1999) Int. Immunol. 11:1965.
  9. Blum, H. et al. (1996) J. Immunol. 157:1846.
  10. Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23.
  11. Mosley, B. et al. (1989) Cell 59:335.
  12. Kondo, M. et al. (1993) Science 262:1874.
  13. Russell, S.M. et al. (1993) Science 262:1880.
  14. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. 93:497.
  15. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  16. Ramalingam, T.R. et al. (2008) Nat. Immunol. 9:25.
  17. Overwijk, W.W. and K.S. Schluns (2009) Clin. Immunol. 132:153.
Long Name
Interleukin 4 Receptor alpha
Entrez Gene IDs
3566 (Human); 16190 (Mouse)
Alternate Names
CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha

Citations for Recombinant Human IL-4R alpha Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Extracellular and intracellular mechanisms of mechanotransduction in three-dimensionally embedded rat chondrocytes.
    Authors: Shioji, Suguru, Imai, Shinji, Ando, Kosei, Kumagai, Kousuke, Matsusue, Yoshitak
    PLoS ONE, 2014;9(12):e114327.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Effect of an interleukin-4 variant on late phase asthmatic response to allergen challenge in asthmatic patients: results of two phase 2a studies.
    Authors: Wenzel S, Wilbraham D, Fuller R, Getz EB, Longphre M
    Lancet, 2007;370(9596):1422-31.
    Species: Human
    Sample Types: Serum
    Applications: Binding Assay
  3. IL-13 receptor alpha 2: a regulator of IL-13 and IL-4 signal transduction in primary human fibroblasts.
    Authors: Andrews AL, Nasir T, Bucchieri F, Holloway JW, Holgate ST, Davies DE
    J. Allergy Clin. Immunol., 2006;118(4):858-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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