Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Catalog # Availability Size / Price Qty
3230-A5-050
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human Integrin alpha 5 beta 1 Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Recombinant Human Integrin alpha 5 beta 1 binds Bovine Fibronectin (Catalog # 1030-FN) coated at 2 µg/mL with an apparent Kd <0.5 nM in the presence of 1 mM MnCl2.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 5 beta 1 protein
Human Integrin alpha 5
(Phe42-Tyr995)
Accession # P08648
acidic tail
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556
basic tail
N-terminus C-terminus
N-terminal Sequence
Analysis
Phe42 (Integrin alpha 5) & No results obtained: Gln21 predicted (Integrin beta 1)
Predicted Molecular Mass
108 kDa ( alpha 5 subunit), 82.3 kDa ( beta 1 subunit)
SDS-PAGE
105-150 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3230-A5

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Integrin alpha 5 beta 1

Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). The human Integrin alpha 5/CD49e cDNA encodes a 1049 amino acid (aa) precursor that includes a 41 aa signal sequence, a 954 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain. The ECD contains seven FG-GAP repeats and nine internal disulfide bonds (3). Within the ECD, human alpha 5 shares 90% and 46% aa sequence identity with mouse alpha 5 and human alpha 8, respectively, and less than 30% aa sequence identitity with other human alpha chains. The human Integrin beta 1/CD29 cDNA encodes a 798 aa precursor that includes a 20 aa signal sequence, a 708 aa ECD, a 23 aa transmembrane segment, and a 47 aa cytoplasmic domain. The ECD contains one vWF-A domain, four Cys-rich repeats, and 29 internal disulfide bonds (3). Five alternate splice forms of the cytoplasmic domain vary by 12 to 48 aa. Within the ECD, human beta 1 shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. It shares 35-45% aa sequence identity with other beta chains. alpha 5/ beta 1 binds fibronectin in both RGD-dependent and -independent manners (4, 5). alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGFR3 and Tie2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including HER2, uPAR, Galectin‑1, CTGF, and thrombin-cleaved Osteopontin (5, 10-13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14, 15).

References
  1. Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
  2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
  4. Takagi, J. et al. (2003) EMBO J. 22:4607.
  5. Wei, Y. et al. (2005) J. Cell Biol. 168:501.
  6. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
  7. Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
  8. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
  9. Cascone, I. et al. (2005) J. Cell Biol. 170:993.
  10. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
  11. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
  12. Gao, R. and D.R. Brigstock (2005) Gut 129:1019
  13. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  14. Carstanjen, D. et al. (2005) Transfusion 45:1192.
  15. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.
Entrez Gene IDs
3678 (Human)
Alternate Names
Integrin alpha 5 beta 1; VLA-5

Citations for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. ITGA5 inhibition in pancreatic stellate cells attenuates desmoplasia and potentiates efficacy of chemotherapy in pancreatic cancer
    Authors: PR Kuninty, R Bansal, SWL De Geus, DF Mardhian, J Schnittert, J van Baarle, G Storm, MF Bijlsma, HW van Laarho, JM Metselaar, PJK Kuppen, AL Vahrmeijer, A Östman, CFM Sier, J Prakash
    Sci Adv, 2019;5(9):eaax2770.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  2. CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is Associated with Alterations in Integrin Binding
    Authors: EC Skoog, VA Morikis, ME Martin, GA Foster, LP Cai, LM Hansen, B Li, JA Gaddy, SI Simon, JV Solnick
    MBio, 2018;9(3):.
    Applications: Bioassay
  3. Systematic site-directed mutagenesis of the Helicobacter pylori CagL protein of the Cag type IV secretion system identifies novel functional domains
    Sci Rep, 2016;6(0):38101.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Developmet
  4. Integrin alpha3beta1 Binding to Fibronectin Is Dependent on the Ninth Type III Repeat.
    Authors: Brown A, Dysart M, Clarke K, Stabenfeldt S, Barker T
    J Biol Chem, 2015;290(42):25534-47.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention.
    Authors: Stampolidis P, Ullrich A, Iacobelli S
    Oncogene, 2015;34(1):39-52.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. Snail modulates the assembly of fibronectin via alpha5 integrin for myocardial migration in zebrafish embryos.
    Authors: Qiao L, Gao H, Zhang T, Jing L, Xiao C, Xiao Y, Luo N, Zhu H, Meng W, Xu H, Mo X
    Sci Rep, 2014;4(0):4470.
    Species: Zebrafish
    Sample Types: Embryo
    Applications: In Vivo
  7. Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice.
    Authors: Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J
    PLoS ONE, 2013;8(11):e81165.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  8. Integrin alpha2beta1 mediates tyrosine phosphorylation of vascular endothelial cadherin induced by invasive breast cancer cells.
    Authors: Haidari M, Zhang W, Caivano A, Chen Z, Ganjehei L, Mortazavi A, Stroud C, Woodside D, Willerson J, Dixon R
    J Biol Chem, 2012;287(39):32981-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. PEGylated liposomal doxorubicin targeted to alpha5beta1-expressing MDA-MB-231 breast cancer cells.
    Authors: Shroff K, Kokkoli E
    Langmuir, 2012;28(10):4729-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Heterodimeric integrin complexes containing beta1-integrin promote internalization and lethality of anthrax toxin.
    Authors: Martchenko M, Jeong SY, Cohen SN
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(35):15583-8.
  11. Efficient delivery of angiostatin K1-5 into tumors following insertion of an NGR peptide into adenovirus capsid.
    Authors: Jullienne B, Vigant F, Muth E, Chaligne R, Bouquet C, Giraudier S, Perricaudet M, Benihoud K
    Gene Ther., 2009;16(12):1405-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Integrin alpha 5 beta 1 Protein, CF
By Anonymous on 04/30/2019
Application: Binding assay/Protein-protein interaction