Recombinant Human Integrin alpha 5 beta 1 Protein, CF

R&D Systems | Catalog # 3230-A5

R&D Systems
Discontinued Product
3230-A5 has been discontinued. An alternative/replacement product is available: 3230-A5B. View all Integrin alpha 5 beta 1 products.

Key Product Details

Source

CHO

Accession Number

P08648 (Integrin alpha 5) & P05556 (Integrin beta 1)

Applications

Binding Activity
View all Integrin alpha 5 beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 5 beta 1 protein
Human Integrin alpha 5
(Phe42-Tyr995)
Accession # P08648		 acidic tail
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556
basic tail
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe42 (Integrin alpha 5) & No results obtained: Gln21 predicted (Integrin beta 1)

Predicted Molecular Mass

108 kDa ( alpha 5 subunit), 82.3 kDa ( beta 1 subunit)

SDS-PAGE

105-150 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Integrin alpha 5 beta 1 binds Bovine Fibronectin (Catalog # 1030-FN) coated at 2 µg/mL with an apparent Kd <0.5 nM in the presence of 1 mM MnCl2.

Reviewed Applications

Read 3 reviews rated 4.3 using 3230-A5 in the following applications:

Formulation, Preparation, and Storage

3230-A5
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 5 beta 1

Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). The human Integrin alpha 5/CD49e cDNA encodes a 1049 amino acid (aa) precursor that includes a 41 aa signal sequence, a 954 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain. The ECD contains seven FG-GAP repeats and nine internal disulfide bonds (3). Within the ECD, human alpha 5 shares 90% and 46% aa sequence identity with mouse alpha 5 and human alpha 8, respectively, and less than 30% aa sequence identitity with other human alpha chains. The human Integrin beta 1/CD29 cDNA encodes a 798 aa precursor that includes a 20 aa signal sequence, a 708 aa ECD, a 23 aa transmembrane segment, and a 47 aa cytoplasmic domain. The ECD contains one vWF-A domain, four Cys-rich repeats, and 29 internal disulfide bonds (3). Five alternate splice forms of the cytoplasmic domain vary by 12 to 48 aa. Within the ECD, human beta 1 shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. It shares 35-45% aa sequence identity with other beta chains. alpha 5/ beta 1 binds fibronectin in both RGD-dependent and -independent manners (4, 5). alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGFR3 and Tie2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including HER2, uPAR, Galectin‑1, CTGF, and thrombin-cleaved Osteopontin (5, 10-13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14, 15).

References

  1. Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
  2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
  4. Takagi, J. et al. (2003) EMBO J. 22:4607.
  5. Wei, Y. et al. (2005) J. Cell Biol. 168:501.
  6. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
  7. Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
  8. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
  9. Cascone, I. et al. (2005) J. Cell Biol. 170:993.
  10. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
  11. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
  12. Gao, R. and D.R. Brigstock (2005) Gut 129:1019
  13. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  14. Carstanjen, D. et al. (2005) Transfusion 45:1192.
  15. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.

Alternate Names

VLA-5

Entrez Gene IDs

3678 (Human)

Gene Symbol

ITGA5

UniProt

P08648 (Integrin alpha 5) & P05556 (Integrin beta 1)

Additional Integrin alpha 5 beta 1 Products

Product Documents for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

For research use only

Citations for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Customer Reviews for Recombinant Human Integrin alpha 5 beta 1 Protein, CF (3)

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  • Recombinant Human Integrin alpha 5 beta 1 Protein, CF
    Name: Andres Ocampo
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/11/2022
    Integrin was used to stimulate a pro-inflammatory activity on rats’ synoviocytes. For this, primary synoviocytes were isolated from rats’ articulations and treated with the integrin. Results showed a pro-inflammatory production of IL-1ra, IL-6, and TNF-a.
    Recombinant Human Integrin alpha 5 beta 1 Protein, CF 3230-A5
  • Recombinant Human Integrin alpha 5 beta 1 Protein, CF
    Name: Amruta Narayanappa
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/27/2020
  • Recombinant Human Integrin alpha 5 beta 1 Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 04/30/2019

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FAQs for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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