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Recombinant Human ITCH/AIP4 Isoform 2 Protein, CF

Catalog #: E3-260
3 Citations Datasheet / COA / SDS

Discontinued Product

E3-260 has been discontinued.
View all ITCH/AIP4 products.
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Citations (3)
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Recombinant Human ITCH/AIP4 Isoform 2 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity

Recombinant Human ITCH/AIP4 is a HECT domain Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human ITCH/AIP4 concentration of 0.1-0.5 μM.

Source
E. coli-derived human ITCH/AIP4 protein
Accession #
Q96J02-2
Predicted Molecular Mass
99 kDa

Product Datasheets

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E3-260

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-260

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol, 2 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ITCH/AIP4

The mammalian Itchy homolog, or ITCH, (also known as Atrophin-1-interacting protein 4 or AIP4) is a HECT domain class ubiquitin E3 ligase. ITCH/AIP4 ubiquitinates the phosphorylated form of Dishevelled protein and promotes its degradation via the Ubiquitin Proteasome System, thereby inhibiting canonical Wnt signaling. The absence of ITCH/AIP4 has been shown to cause severe autoimmune disease in mice. Recent studies have identified multisystem autoimmune disease and morphologic and developmental abnormalities in human patients with ITCH/AIP4 deficiency, thus underscoring the importance of ITCH/AIP4 in many cellular processes. This ligase may catalyze K29-, K48-, and/or K63-linked polyubiquitin chain formation on a variety of reported targets. This untagged, ITCH/AIP4 isoform 2 recombinant protein demonstrates strong autoubiquitination activity in vitro.

References
  1. Wei W., et al. (2012) Mol. Cell Biol. 32: 3903-12
  2. Lohr N.J., et al. (2010) Am. J. Hum. Genet. 86: 447-453
  3. Yang C., et al. (2006) Mol. Cell 21: 135-141
Long Name
Itchy E3 Ubiquitin Protein Ligase
Entrez Gene IDs
83737 (Human); 16396 (Mouse); 311567 (Rat)
Alternate Names
AIF4; AIP4; AIP4dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)); atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; EC 6.3.2; EC 6.3.2.-; ITCH; itchy (mouse homolog) E3 ubiquitin protein ligase; itchy E3 ubiquitin protein ligase homolog (mouse); itchy homolog E3 ubiquitin protein ligase; NAPP1; NAPP1E3 ubiquitin-protein ligase Itchy homolog; NFE2-associated polypeptide 1; ubiquitin protein ligase ITCH

Citations for Recombinant Human ITCH/AIP4 Isoform 2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Cdk5-mediated JIP1 phosphorylation regulates axonal outgrowth through Notch1 inhibition
    Authors: DS Im, A Joselin, D Svoboda, T Takano, MWC Rousseaux, S Callaghan, RS Slack, SI Hisanaga, RJ Davis, DS Park, D Qu
    Bmc Biology, 2022-05-17;20(1):115.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  2. Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes
    Authors: S Yoon, K Bogdanov, D Wallach
    Cell Death and Differentiation, 2022-01-09;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  3. The Itch ubiquitin ligase is required for KSHV RTA induced vFLIP degradation
    Authors: Jennifer C Chmura
    Virology, 2016-11-30;501(0):119-126.
    Applications: Bioassay

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