Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI1277-050
Biotinylated Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein Binding Activity.
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Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Notch‑1 Fc Chimera (Catalog # 3647-TK) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein binds with an ED50 of 0.500-2.50 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Jagged 1 protein
Human Jagged 1
(Ser32-Ser1046)
Accession # P78504.3
IEGRMDHuman IgG1
(Pro100-Lys330)
Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ser32
Structure / Form
Disulfide-linked homodimer
Biotinylated via Avi-tag
Predicted Molecular Mass
139 kDa
SDS-PAGE
150-170 kDa, under reducing conditions.

Product Datasheets

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AVI1277

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI1277

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human Notch‑1 Fc Chimera (3647-TK) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein (Catalog # AVI1277) binds with an ED50 of 0.500-2.50 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human Jagged 1 Fc Chimera Avi-tag Protein (Catalog # AVI1277) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 150-170 kDa and 300-340 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10). Our Avi-tag Biotinylated Human Jagged 1 Fc Chimera protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.
Entrez Gene IDs
182 (Human); 16449 (Mouse); 29146 (Rat)
Alternate Names
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

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