Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF

R&D Systems | Catalog # 3015-KR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein (3015-KR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P43627

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all KIR2DL2/CD158b1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human KIR2DL2/CD158b1 protein
Human KIR2DL2
(His22-His245)
Accession # P43627		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His22

Predicted Molecular Mass

51.2 kDa (monomer)

SDS-PAGE

65-75 kDa, reducing conditions

Activity

Bioassay data are not available.

Reviewed Applications

Read 3 reviews rated 4.3 using 3015-KR in the following applications:

Formulation, Preparation, and Storage

3015-KR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR2DL2/CD158b1

KIR2DL2 (2DL2, formerly NKAT6, designated CD158b) is a 348 amino acid (aa) type I transmembrane glycoprotein that belongs to the human killer cell Ig‑like receptor (KIR) family (1, 2). KIRs are expressed on human CD56dim NK cells and T cell subsets, and regulate effector functions in the innate immune system (1‑3). KIRs are named for the number of Ig‑like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails (1‑3). Individuals will express varying subsets of inhibiting and activating KIRs with varying polymorphisms (1, 4). Like other inhibiting KIRs, KIR2DL2 has two ITIM domains within its long tail that block activating receptor clustering (2, 5). Within the ECD, KIR2DL2 shares very high aa sequence identity (98%) with KIR2DL3. The two segregate as alleles of the same gene, although KIR2DL2 shows higher avidity for HLA‑C1 ligands (1, 6). Extracellular aa identity is also high for KIR2DL1 (92%). The three together recognize and inhibit NK cytotoxicity against cells expressing any HLA‑C allotype, allowing self‑recognition, but also conferring susceptibility to leukemia (1‑3). KIR2DL2 recognizes Asn80‑containing HLA‑C1 and, more weakly, Lys80‑containing C2 allotypes (1, 6‑8). KIR2DL2 can impact both innate and adaptive immunity, contributing to either viral persistence or antiviral immunity, depending on the HLA class I molecules expressed by the individual (9).

References

  1. Purdy, A.K. and Campbell, K.S. (2009) Cancer Biol. Ther. 8:13.
  2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  3. Kulkarni, S. et al. (2008) Sem. Immunol. 20:343.
  4. Middleton, D. and F. Gonzelez (2009) Immunology 129:8.
  5. Abeyweera, T.P. et al. (2011) J. Cell Biol. 192:675.
  6. Moesta, A.K. et al. (2008) J. Immunol. 180:3969.
  7. Boyington, J.C. et al. (2000) Nature 405:537.
  8. Snyder, G.A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3864.
  9. Seich al Basatena, N-K. et al. (2011) PLoS Pathog. 7:e1002270.

Long Name

Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 2

Alternate Names

CD158b1, CL-43, NKAT6

Entrez Gene IDs

3803 (Human)

Gene Symbol

KIR2DL2

UniProt

P43627

Additional KIR2DL2/CD158b1 Products

Product Documents for Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF

Customer Reviews for Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF (3)

4.3 out of 5
3 Customer Ratings
5 Stars
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  • Name: Sivanesan Dakshanamurthy
    Application: SPR assay
    Verified Customer | Posted 12/06/2018
  • Name: Sivanesan Dakshanamurthy
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/30/2018
  • Name: Sivanesan Dakshanamurthy
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/30/2018

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