Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
3015-KR-050
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (3)

Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind HEK293T human embryonic kidney cells in a flow cytometry assay. When 1 μg of Recombinant Human KIR2DL2/CD158b1 Fc Chimera is added to 5 x 105 HEK293T cells, >20% of the cells will bind to the protein.
Source
Mouse myeloma cell line, NS0-derived human KIR2DL2/CD158b1 protein
Human KIR2DL2
(His22 - His245)
Accession # P43627
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
His22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51.2 kDa (monomer)
SDS-PAGE
65-75 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3015-KR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: KIR2DL2/CD158b1

KIR2DL2 (2DL2, formerly NKAT6, designated CD158b) is a 348 amino acid (aa) type I transmembrane glycoprotein that belongs to the human killer cell Ig‑like receptor (KIR) family (1, 2). KIRs are expressed on human CD56dim NK cells and T cell subsets, and regulate effector functions in the innate immune system (1 ‑ 3). KIRs are named for the number of Ig‑like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails (1 ‑ 3). Individuals will express varying subsets of inhibiting and activating KIRs with varying polymorphisms (1, 4). Like other inhibiting KIRs, KIR2DL2 has two ITIM domains within its long tail that block activating receptor clustering (2, 5). Within the ECD, KIR2DL2 shares very high aa sequence identity (98%) with KIR2DL3. The two segregate as alleles of the same gene, although KIR2DL2 shows higher avidity for HLA‑C1 ligands (1, 6). Extracellular aa identity is also high for KIR2DL1 (92%). The three together recognize and inhibit NK cytotoxicity against cells expressing any HLA‑C allotype, allowing self‑recognition, but also conferring susceptibility to leukemia (1 ‑ 3). KIR2DL2 recognizes Asn80‑containing HLA‑C1 and, more weakly, Lys80‑containing C2 allotypes (1, 6 ‑ 8). KIR2DL2 can impact both innate and adaptive immunity, contributing to either viral persistence or antiviral immunity, depending on the HLA class I molecules expressed by the individual (9).

References
  1. Purdy, A.K. and Campbell, K.S. (2009) Cancer Biol. Ther. 8:13.
  2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  3. Kulkarni, S. et al. (2008) Sem. Immunol. 20:343.
  4. Middleton, D. and F. Gonzelez (2009) Immunology 129:8.
  5. Abeyweera, T.P. et al. (2011) J. Cell Biol. 192:675.
  6. Moesta, A.K. et al. (2008) J. Immunol. 180:3969.
  7. Boyington, J.C. et al. (2000) Nature 405:537.
  8. Snyder, G.A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3864.
  9. Seich al Basatena, N-K. et al. (2011) PLoS Pathog. 7:e1002270.
Long Name
Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 2
Entrez Gene IDs
3803 (Human)
Alternate Names
CD158b1; CL-43; KIR2DL2; NKAT6

Citations for Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. HLA-C-restricted viral epitopes are associated with an escape mechanism from KIR2DL2+ NK cells in Lassa virus infection
    Authors: N Wauquier, C Petitdeman, N Tarantino, C Maucourant, M Coomber, V Lungay, J Bangura, P Debré, V Vieillard
    EBioMedicine, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Peptide selectivity discriminates NK cells from KIR2DL2- and KIR2DL3-positive individuals.
    Authors: Cassidy S, Mukherjee S, Myint T, Mbiribindi B, North H, Traherne J, Mulder A, Claas F, Purbhoo M, Das J, Khakoo S
    Eur J Immunol, 2015;45(2):492-500.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  3. HLA-Cw*0102-Restricted HIV-1 p24 Epitope Variants Can Modulate the Binding of the Inhibitory KIR2DL2 Receptor and Primary NK Cell Function.
    Authors: Fadda L, Korner C, Kumar S, van Teijlingen NH, Piechocka-Trocha A, Carrington M, Altfeld M
    PLoS Pathog., 2012;8(7):e1002805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF

Average Rating: 4.3 (Based on 3 Reviews)

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Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF
By Anonymous on 12/06/2018
Application: SPR assay
Reason for Rating: good quality

Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF
By Anonymous on 11/30/2018
Application: Binding assay/Protein-protein interaction

Recombinant Human KIR2DL2/CD158b1 Fc Chimera Protein, CF
By Anonymous on 11/30/2018
Application: Binding assay/Protein-protein interaction