Recombinant Human KIR3DL2/CD158k Fc Chimera Protein, CF

(2 citations)   
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to bind HLA on MDA‑MB‑231 human breast cancer cells. The ED50 for this effect is 0.06-0.36 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived
    Human KIR3DL2
    (Leu22-Leu339)
    Accession # P43630
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu22
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    61.4 kDa (monomer)
  • SDS-PAGE
    85-90 kDa, reducing conditions
2878-KR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human KIR3DL2/CD158k Fc Chimera (Catalog #
2878-KR)binds to
MDA-MB-231 human breast cancer cells. The ED50 for this effect is0.06-0.36 μg/mL.
Background: KIR3DL2/CD158k

KIR3DL2 (also known as 3DL2, p140, CD158k) is a type I transmembrane protein of the p70 family of killer cell Ig-like receptors (KIR). KIR are expressed on CD56dim NK cells and T cell subsets where they participate in identifying normal and abnormal cells and regulating effector functions of the innate immune system (1 - 4). KIR are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD) and whether they have long or short (L, S) cytoplasmic tail. As with other inhibitory KIR, KIR3DL2 has two ITIM domains within its long tail (3). KIR3DL2 is diverse, with twelve alleles identified and as many as five single amino acid (aa) polymorphisms found in a single individual (4 - 6). Unlike most other KIR, gene transcripts of KIR3DL2 are expressed by all individuals (4). KIR3DL2 is present on the cell surface as a disulfide-linked homodimer of two 70 kDa, 434 aa subunits (4). KIR3DL2 has shown peptide-specific binding to some HLA-A antigens, including A3 and A11 (4, 7, 8). It also binds the abnormally folded HLA-B27 homodimer found in spondylarthritides, but not the normal heterodimer of HLA-B27 with beta 2-microglobulin (9, 10). NK and CD4+ T cells from patients with spondylarthritides show increased KIR3DL2+ expression and this may play a role in disease pathology (10). KIR3DL2 is also a marker for atypical mononuclear (Sezary) cells in the blood of patients with Sezary syndrome, an erythrodermic form of cutaneous T cell lymphoma (11). Human KIR3DL2 ECD shows 88 - 92% aa identity to KIR3DL2 of other primates. KIR receptors have no structural orthologs in non-primates, although mouse Ly-49 proteins are functional orthologs (3). KIR are highly related. The closest relative, KIR3DL1 shows 86% aa identity with KIR3DL2 within the ECD.

  • References:
    1. Colonna, M. and J. Samaridis (1995) Science 268:405.
    2. Pende, D. et al. (1996) J. Exp. Med. 184:505.
    3. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
    4. Uhrberg, M. et al. (1997) Immunity 7:753.
    5. Meenaugh, A. et al. (2004) Tissue Antigens 64:226.
    6. Kwon, D. et al. (2000) Mol. Cells 10:54.
    7. Dohring, C. et al. (1996) J. Immunol. 156:3098.
    8. Hansasuta, P. et al. (2004) Eur. J. Immunol. 34:1673.
    9. Kollnberger, S. et al. (2002) Arthritis Rheum. 46:2972.
    10. Chan, A. T. et al. (2005) Arthritis Rheum. 52:3586.
    11. Poszepczynska-Guigne, E. et al. (2004) J. Invest. Dermatol. 122:820.
  • Long Name:
    Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 2
  • Entrez Gene IDs:
    3812 (Human)
  • Alternate Names:
    killer cell immunoglobulin-like receptor 3DL2; CD158k; CL-5; killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 2; KIR3DL2; NKAT4; NKAT-4; NKAT4A; NKAT4B; p140
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. High affinity anti-TIM-3 and anti-KIR monoclonal antibodies cloned from healthy human individuals
    Authors: S Ryser, A Estellés, E Tenorio, LM Kauvar, ML Gishizky
    PLoS ONE, 2017;12(7):e0181464.
    Species: Human
    Sample Type: Recombinant Proteins
    Application: Bioassay
  2. HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats.
    Authors: Marroquin Belaunzaran O, Kleber S, Schauer S, Hausmann M, Nicholls F, van den Broek M, Payeli S, Ciurea A, Milling S, Stenner F, Shaw J, Kollnberger S, Bowness P, Petrausch U, Renner C
    PLoS ONE, 2015;10(6):e0130811.
    Species: Human
    Sample Type: Protein
    Application: Bioassay
Recombinant Proteins
Description Application Cat# Citations Images  

Recombinant Human IgG1 Fc, CF

BA 110-HG 66
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