Recombinant Human KIR3DL2/CD158k Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
2878-KR-050
Recombinant Human KIR3DL2/CD158k Fc Chimera Protein Bioactivity
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Product Details
Citations (2)
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Reviews (2)

Recombinant Human KIR3DL2/CD158k Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind HLA on MDA‑MB‑231 human breast cancer cells. The ED50 for this effect is 0.06-0.36 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human KIR3DL2/CD158k protein
Human KIR3DL2
(Leu22-Leu339)
Accession # P43630
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
61.4 kDa (monomer)
SDS-PAGE
85-90 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2878-KR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human KIR3DL2/CD158k Fc Chimera Protein Bioactivity View Larger

Recombinant Human KIR3DL2/CD158k Fc Chimera (Catalog # 2878-KR) binds to MDA-MB-231 human breast cancer cells. The ED50 for this effect is 0.06-0.36 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: KIR3DL2/CD158k

KIR3DL2 (also known as 3DL2, p140, CD158k) is a type I transmembrane protein of the p70 family of killer cell Ig-like receptors (KIR). KIR are expressed on CD56dim NK cells and T cell subsets where they participate in identifying normal and abnormal cells and regulating effector functions of the innate immune system (1 - 4). KIR are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD) and whether they have long or short (L, S) cytoplasmic tail. As with other inhibitory KIR, KIR3DL2 has two ITIM domains within its long tail (3). KIR3DL2 is diverse, with twelve alleles identified and as many as five single amino acid (aa) polymorphisms found in a single individual (4 - 6). Unlike most other KIR, gene transcripts of KIR3DL2 are expressed by all individuals (4). KIR3DL2 is present on the cell surface as a disulfide-linked homodimer of two 70 kDa, 434 aa subunits (4). KIR3DL2 has shown peptide-specific binding to some HLA-A antigens, including A3 and A11 (4, 7, 8). It also binds the abnormally folded HLA-B27 homodimer found in spondylarthritides, but not the normal heterodimer of HLA-B27 with beta 2-microglobulin (9, 10). NK and CD4+ T cells from patients with spondylarthritides show increased KIR3DL2+ expression and this may play a role in disease pathology (10). KIR3DL2 is also a marker for atypical mononuclear (Sezary) cells in the blood of patients with Sezary syndrome, an erythrodermic form of cutaneous T cell lymphoma (11). Human KIR3DL2 ECD shows 88 - 92% aa identity to KIR3DL2 of other primates. KIR receptors have no structural orthologs in non-primates, although mouse Ly-49 proteins are functional orthologs (3). KIR are highly related. The closest relative, KIR3DL1 shows 86% aa identity with KIR3DL2 within the ECD.

References
  1. Colonna, M. and J. Samaridis (1995) Science 268:405.
  2. Pende, D. et al. (1996) J. Exp. Med. 184:505.
  3. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  4. Uhrberg, M. et al. (1997) Immunity 7:753.
  5. Meenaugh, A. et al. (2004) Tissue Antigens 64:226.
  6. Kwon, D. et al. (2000) Mol. Cells 10:54.
  7. Dohring, C. et al. (1996) J. Immunol. 156:3098.
  8. Hansasuta, P. et al. (2004) Eur. J. Immunol. 34:1673.
  9. Kollnberger, S. et al. (2002) Arthritis Rheum. 46:2972.
  10. Chan, A. T. et al. (2005) Arthritis Rheum. 52:3586.
  11. Poszepczynska-Guigne, E. et al. (2004) J. Invest. Dermatol. 122:820.
Long Name
Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 2
Entrez Gene IDs
3812 (Human)
Alternate Names
killer cell immunoglobulin-like receptor 3DL2; CD158k; CL-5; killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 2; KIR3DL2; NKAT4; NKAT-4; NKAT4A; NKAT4B; p140

Citations for Recombinant Human KIR3DL2/CD158k Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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  1. High affinity anti-TIM-3 and anti-KIR monoclonal antibodies cloned from healthy human individuals
    Authors: S Ryser, A Estellés, E Tenorio, LM Kauvar, ML Gishizky
    PLoS ONE, 2017;12(7):e0181464.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats.
    Authors: Marroquin Belaunzaran O, Kleber S, Schauer S, Hausmann M, Nicholls F, van den Broek M, Payeli S, Ciurea A, Milling S, Stenner F, Shaw J, Kollnberger S, Bowness P, Petrausch U, Renner C
    PLoS ONE, 2015;10(6):e0130811.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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Reviews for Recombinant Human KIR3DL2/CD158k Fc Chimera Protein, CF

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Recombinant Human KIR3DL2/CD158k Fc Chimera Protein, CF
By Anonymous on 12/06/2018
Application: SPR assay
Reason for Rating: good quality

Recombinant Human KIR3DL2/CD158k Fc Chimera Protein, CF
By Anonymous on 11/30/2018
Application: Binding assay/Protein-protein interaction