Recombinant Human KIR3DS1/CD158e2 Fc Chimera Protein, CF

(1 citations)   
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to bind HLA on MDA‑MB‑231 human breast cancer cells. The ED50 for this effect is typically 0.03-0.18 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Human KIR3DS1
    Accession #Q14943
    IEGRMD Human IgG1
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
  • Predicted Molecular Mass
    61.9 (monomer) kDa
    75-90 kDa, reducing conditions
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human KIR3DS1/CD158e2 Fc Chimera (Catalog # 4136-KR)binds to
MDA-MB-231 human breast cancer cells. The ED50 for this effect istypically
0.03-0.18 μg/mL.
Background: KIR3DS1/CD158e2

KIR3DS1 (3DS1, CD158e2) is a type I transmembrane protein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56dim NK cells and T cell subsets where they differentiate normal from abnormal cells, and regulate effector functions in the innate immune system (1 - 3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. Like other activating KIRs, KIR3DS1 has a short cytoplasmic tail and a positively charged amino acid (aa) within the transmembrane domain that interacts with the ITAM-bearing signaling adaptor, DAP12 (2, 4). Crosslinking of KIR3DS1 activates cytolysis and induces IFN-gamma production, confirming it to be an activating receptor (4). Approximately 38% of the population expresses KIR3DS1 on the surface of NK cells (1, 4, 5). Variants lacking the N-terminal Ig-like domain and/or with substitutions in the cytoplasmic tail have been described (1, 6). The 50 kDa, 387 aa KIR3DS1 shows 97% aa identity with KIR3DL1 within the ECD, and the two segregate as alleles (3, 7). Some activating KIRs bind weakly to the ligands recognized by their corresponding inhibitory KIR. KIR3DS1 does not bind appreciably to cells transfected with ligands for HLA-Bw4 KIR3DL1 (4, 5). However, HIV-infected people who express the combined phenotype of KIR3DS1 with Bw4 alleles that contain an isoleucine at aa 80, show delayed progression to AIDS and fewer AIDS-related opportunistic infections (7, 8). KIR receptors have no structural orthologs in nonprimates, although mouse Ly-49 proteins perform similar functions (2).

  • References:
    1. Dohring, C. et al. (1996) Immunogenetics 44:227.
    2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
    3. Uhrberg, M. et al. (1997) Immunity 7:753.
    4. Carr, W. H. et al. (2007) J. Immunol. 178:647.
    5. O’Connor, G. M. et al. (2007) J. Immunol. 178:235.
    6. Valiante, N. M. et al. (1997) Immunity 7:739.
    7. Martin, M. P. et al. (2002) Nat. Genet. 31:429.
    8. Qi, Y. et al. (2006) PloS Pathog. 2:e79.
  • Long Name:
    Killer Cell Immunoglobulin-like Receptor, Three Domains, Long Cytoplasmic Tail, 1
  • Entrez Gene IDs:
    3813 (Human)
  • Alternate Names:
    CD158e2; KIR-G1; nkat10
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. HLA-Cw*0102-Restricted HIV-1 p24 Epitope Variants Can Modulate the Binding of the Inhibitory KIR2DL2 Receptor and Primary NK Cell Function.
    Authors: Fadda L, Korner C, Kumar S, van Teijlingen NH, Piechocka-Trocha A, Carrington M, Altfeld M
    PLoS Pathog., 2012;8(7):e1002805.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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