Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF

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Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human R‑Spondin 3 (Catalog # 3500-RS/CF) is coated at 0.5 μg/mL, Recombinant Human Lgr5/GPR49 Fc Chimera binds with an apparent Kd <0.1 nM.  Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.5-7.5 µg/mL in the presence of 20 ng/mL Recombinant Human R‑Spondin 2 (Catalog # 3266-RS) and 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Chinese Hamster Ovary cell line, CHO-derived human Lgr5/GPR49 protein
Human LGR-5/GPR49
Accession # O75473
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
87 kDa (monomer)
90-110 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Lgr5/GPR49

Lgr5 (leucine-rich repeat G-protein coupled receptor 5), also called GPR49, is a seven-transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1, 2). While this family includes receptors for hormones such as LH, FSH, TSH, and HCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G-protein independent mediators of the potentiating effect of R-Spondins on Wnt signaling (1-6). Lgr5 binds and forms complexes with R-Spondins, Frizzled Wnt receptors and LRP Wnt coreceptors (3-5). It acts at least in part by enhancing Wnt-dependent LRP phosphorylation, internalization of LRPs, and accumulation of beta -catenin (3-5). Human Lgr5 cDNA encodes 907 amino acids (aa), including a long N-terminal extracellular domain (ECD, aa 22-561) with 16-17 LRR domains that mediate ligand interaction (2). The LRR-containing ECD of human Lgr5 shares 90% aa sequence identity with mouse and rat Lgr4, and 50-60% aa identity with human Lgr4 and Lgr6. Lgr5 is expressed by embryonic and adult epithelial stem cells, especially in the intestines, stomach, hair follicles, inner ear and mammary glands (1, 5, 6). Lgr4 is also present in epithelial stem cells, but shows wider expression elsewhere, making Lgr5 the preferred marker for these cells (5). Lgr5+ stem cells are sufficient to produce all epithelial cell types of the intestinal crypts in vivo or in vitro, when supported by secreted factors from neighboring Paneth or goblet cells (1, 7). In the embryo, Lgr5 is involved in development of the kidney and craniofacial structures (1). It is up-regulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas, and may promote carcinogenesis (6).

  1. Barker, N. et al. (2013) Development 140:2484.
  2. Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.
  3. Carmon, K.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:11452.
  4. Glinka, A. et al. (2011) EMBO Rep. 12:1055.
  5. de Lau, W. et al. (2011) Nature 476:293.
  6. Leushacke, M. and N. Barker (2012) Oncogene 31:3009.
  7. Sato, T. et al. (2011) Nature 469:415.
Long Name
Leucine-rich Repeat Containing G Protein-coupled Receptor 5
Entrez Gene IDs
8549 (Human); 14160 (Mouse); 299802 (Rat); 102120216 (Cynomolgus Monkey)
Alternate Names
FEX; G protein-coupled receptor 49; GPR49; GPR49G-protein coupled receptor HG38; GPR67; G-protein coupled receptor 49; G-protein coupled receptor 67; GRP49; HG38; leucine-rich repeat containing G protein-coupled receptor 5; leucine-rich repeat-containing G protein-coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; Lgr5; MGC117008; orphan G protein-coupled receptor HG38

Citation for Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Clostridioides difficile infection damages colonic stem cells via TcdB, impairing epithelial repair and recovery from disease
    Authors: SJ Mileto, T Jardé, KO Childress, JL Jensen, AP Rogers, G Kerr, ML Hutton, MJ Sheedlo, SC Bloch, JA Shupe, K Horvay, T Flores, R Engel, S Wilkins, PJ McMurrick, DB Lacy, HE Abud, D Lyras
    Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture


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Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF
By Anonymous on 06/09/2020
Application: ELISA standard

We used this protein in an in-house ELISA along with mAb & pAb's from Santacruz to quantify Lgr5 in human serum and plasma. This combination could not detect Lgr5 in our samples.