Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF Summary
Accession # O75473
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Lgr5 (leucine-rich repeat G-protein coupled receptor 5), also called GPR49, is a seven-transmembrane glycoprotein receptor in the Lgr family of cell surface receptors (1, 2). While this family includes receptors for hormones such as LH, FSH, TSH, and HCG, the subfamily comprising Lgr4, Lgr5, and Lgr6 are G-protein independent mediators of the potentiating effect of R-Spondins on Wnt signaling (1-6). Lgr5 binds and forms complexes with R-Spondins, Frizzled Wnt receptors and LRP Wnt coreceptors (3-5). It acts at least in part by enhancing Wnt-dependent LRP phosphorylation, internalization of LRPs, and accumulation of beta -catenin (3-5). Human Lgr5 cDNA encodes 907 amino acids (aa), including a long N-terminal extracellular domain (ECD, aa 22-561) with 16-17 LRR domains that mediate ligand interaction (2). The LRR-containing ECD of human Lgr5 shares 90% aa sequence identity with mouse and rat Lgr4, and 50-60% aa identity with human Lgr4 and Lgr6. Lgr5 is expressed by embryonic and adult epithelial stem cells, especially in the intestines, stomach, hair follicles, inner ear and mammary glands (1, 5, 6). Lgr4 is also present in epithelial stem cells, but shows wider expression elsewhere, making Lgr5 the preferred marker for these cells (5). Lgr5+ stem cells are sufficient to produce all epithelial cell types of the intestinal crypts in vivo or in vitro, when supported by secreted factors from neighboring Paneth or goblet cells (1, 7). In the embryo, Lgr5 is involved in development of the kidney and craniofacial structures (1). It is up-regulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas, and may promote carcinogenesis (6).
- Barker, N. et al. (2013) Development 140:2484.
- Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1830.
- Carmon, K.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:11452.
- Glinka, A. et al. (2011) EMBO Rep. 12:1055.
- de Lau, W. et al. (2011) Nature 476:293.
- Leushacke, M. and N. Barker (2012) Oncogene 31:3009.
- Sato, T. et al. (2011) Nature 469:415.
Citation for Recombinant Human Lgr5/GPR49 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Clostridioides difficile infection damages colonic stem cells via TcdB, impairing epithelial repair and recovery from disease
Authors: SJ Mileto, T Jardé, KO Childress, JL Jensen, AP Rogers, G Kerr, ML Hutton, MJ Sheedlo, SC Bloch, JA Shupe, K Horvay, T Flores, R Engel, S Wilkins, PJ McMurrick, DB Lacy, HE Abud, D Lyras
Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.
Sample Types: Whole Cells
Applications: Cell Culture
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Average Rating: 4 (Based on 1 Review)
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We used this protein in an in-house ELISA along with mAb & pAb's from Santacruz to quantify Lgr5 in human serum and plasma. This combination could not detect Lgr5 in our samples.