Recombinant Human LRRTM4 Protein, CF

Catalog # Availability Size / Price Qty
5377-LR-050
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Recombinant Human LRRTM4 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When rhLRRTM4 is coated at 5 μg/mL (100 μL/well), rhNeurexin 1 beta /Fc Chimera (Catalog # 5268-NX) binds with an apparent KD <15 nM.
Source
Mouse myeloma cell line, NS0-derived human LRRTM4 protein
Gln31-Lys424, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln31 predicted
Predicted Molecular Mass
46.0 kDa
SDS-PAGE
60-70 kDa, reducing conditions

Product Datasheets

5377-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: LRRTM4

LRRTM4 (Leucine-rich repeat transmembrane protein 4) is a member of the LRRTM family of molecules (1). All LRRTMs are type I transmembrane proteins that contain multiple leucine rich repeats, one PDZ consensus cytoplasmic binding domain, and show expression somewhere in the central nervous system. The LRRTM family is iterated across vertebrate (but not invertebrate) species. Human LRRTM4 is synthesized as a 590 amino acid (aa) precursor that contains a 30 aa signal sequence plus a 560 aa mature region. The mature molecule contains a 394 aa extracellular domain (aa 31 ‑ 424), a 21 aa transmembrane segment, and a 105 aa cytoplasmic region (1, 2). The extracellular domain is characterized by the presence of ten Leu-rich repeats, flanked by two cysteine-rich flanking sequences. Based on other LRRTMs, eukaryotic expression of LRRTM4 will likely yield an 82 ‑ 86 kDa glycosylated molecule (3, 4). There are at least two splice variants. Both show a Val substitution for aa 518 ‑ 590, one of which is also coupled to an alternative start site at Met312 (5, 6). Mature human LRRTM4 is 95% aa identical to mouse LRRTM4 (1). LRRTM4 is widely expressed, occurring in embryonic distal limb bud mesenchyme plus anterior sclerotome, and in adult testis, lung, muscle and prostate (1, 7). All LRRTMs are also found in brain. LRRTM4 has been identified in adult granular and mitral neurons of the olfactory bulb, neurons of cerebral cortex layers 2, 4, 5 and 6, and on neurons of the dentate gyrus (1). Functionally, it appears that LRRTM4 is involved in both pre-and post-synaptic modeling. Although embedded in the postsynaptic membrane, LRRTM4 seems to induce excitatory/glutamatergic presynaptic differentiation. Postsynaptically, LRRTM4 likely facilitates excitatory synapse formation on dendrites, acting in conjunction with multiple ligand-receptor combinations. In particular, the NR1 NMDA subunit has been shown to coalesce, or aggregate, in regions that experience LRRTM4 clustering (3, 8).

References
  1. Lauren, J. et al. (2003) Genomics 81:411.
  2. SwissProt # Q86VH4.
  3. Linhoff, M.W. et al. (2009) Neuron 61:734.
  4. Majercak, J. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17967.
  5. GenBank Accession # EAW99585.
  6. GenBank Accession # AAH17769.
  7. Haines, B.P. & P. Rigby (2007) Gene Expr. Patterns 7:23.
  8. Brose, N. (2009) Neuron 61:650.
Long Name
Leucine Rich Repeat Transmembrane Neuronal 4
Entrez Gene IDs
80059 (Human); 500219 (Rat)
Alternate Names
FLJ12568; leucine rich repeat transmembrane neuronal 4; leucine-rich repeat transmembrane neuronal 4 protein; leucine-rich repeat transmembrane neuronal protein 4; LRRTM4; MGC120633; MGC120636

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