Recombinant Human MBL Protein, CF

Catalog # Availability Size / Price Qty
9086-MB-050
Recombinant Human MBL Protein, CF Bioactivity
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Product Details
Citations (6)
FAQs
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Reviews (1)

Recombinant Human MBL Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Mannan is immobilized at 0.1 µg/mL (100 µL/well), the concentration of Recombinant Human MBL that produces 50% of the optimal binding response is approximately 25-150 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human MBL protein
Glu21-Ile248
Accession #
N-terminal Sequence
Analysis
Glu21
Predicted Molecular Mass
24 kDa
SDS-PAGE
31-36 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9086-MB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

When Mannan is coated at 0.1 µg/mL (100 µL/well), Recombinant Human MBL (Catalog # 9086-MB) binds with a typical ED50of 25-150 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: MBL

Human mannose/mannan-binding lectin (MBL) is a 25-30 kDa secreted glycoprotein in the collectin family of pattern-recognition molecules (1, 2). Mature human MBL contains a cysteine-rich region, a collagen-like segment, and a C-type lectin domain that binds to neutral bacterial carbohydrates (3). Human MBL shares 61% amino acid sequence identity with mouse and rat MBL and carries variable post-translation modifications including O-glycosylation and proline and lysine hydroxylation (4). Its collagen-like region mediates MBL association into disulfide-stabilized trimers which further associate into complexes containing three or four copies of the basic trimer (4, 5). MBL is secreted by hepatocytes and opsonizes bacteria through interactions with microbial carbohydrates (5). Tetrameric complexes of MBL show greater carbohydrate binding capacity compared to the trimers (5). MBL multimers can associate with the serine proteases MASP-1, -2, and -3 and promote their cleavage of Complement Component C3 (5-8). Proteolytic cleavage of C3 triggers activation of the complement system and formation of the membrane attack complex, leading to destruction of opsonized bacteria (2). In addition, MBL binds to the scavenger receptor CD91 which mediates the clearance of apoptotic material (9).

References
  1. Scorza, M. et al. (2015) Clin. Chim. Acta 451:78.
  2. Matsushita, M. et al. (2013) Arch. Immunol. Ther. Exp. (Warsz.) 61:273.
  3. Ezekowitz, R.A.B. et al. (1988) J. Exp. Med. 167:1034.
  4. Jensen, P.H. et al. (2005) J. Biol. Chem. 280:11043.
  5. Teillet, F. et al. (2005) J. Immunol. 174:2870.
  6. Moller-Kristensen, M. et al. (2007) Int. Immunol. 19:141.
  7. Thiel, S. et al. (2000) J. Immunol. 165:878.
  8. Vorup-Jensen, T. et al. (2000) J. Immunol. 165:2093.
  9. Duus, K. et al. (2010) FEBS J. 277:4956.
Long Name
Mannose Binding Lectin
Entrez Gene IDs
4153 (Human)
Alternate Names
COLEC1; COLEC1collectin-1; Collectin-1; HSMBPC; Mannan-binding protein; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin (protein C) 2, soluble; Mannose-binding lectin; mannose-binding protein C; MBL; MBL2; MBL2D; MBLmannan-binding lectin; MBP; MBP1; MBP1mannose-binding lectin 2, soluble (opsonic defect); MBP-C; MGC116832; MGC116833

Citations for Recombinant Human MBL Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. The role of Mannose Binding Lectin in the immune response against Borrelia burgdorferi sensu lato
    Authors: J Coumou, A Wagemakers, S Narasimhan, TJ Schuijt, JI Ersoz, A Oei, OJ de Boer, JJTH Roelofs, E Fikrig, JW Hovius
    Sci Rep, 2019;9(1):1431.
    Species: Parasite - Borrelia burgdorferi
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Highly modified and immunoactive N-glycans of the canine heartworm
    Authors: F Martini, B Eckmair, S Štefani?, C Jin, M Garg, S Yan, C Jiménez-Ca, A Hykollari, C Neupert, L Venco, D Varón Silv, IBH Wilson, K Paschinger
    Nat Commun, 2019;10(1):75.
    Species: Parasite - Dirofilaria immitis (Heartworm)
    Sample Types: Glycans
    Applications: Binding Assay
  3. Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma
    Authors: J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani
    PLoS ONE, 2019;14(7):e0219480.
    Species: Human
    Sample Types: Natural Protein
    Applications: Bioassay
  4. Mannose-Binding Lectin: A Potential Therapeutic Candidate against Candida Infection
    Authors: NM Hammad, NE El Badawy, HA Ghramh, LM Al Kady
    Biomed Res Int, 2018;2018(0):2813737.
    Species: Yeast
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Ross River virus envelope glycans contribute to disease through activation of the host complement system
    Authors: BM Gunn, JE Jones, RS Shabman, AC Whitmore, S Sarkar, LK Blevins, TE Morrison, MT Heise
    Virology, 2018;515(0):250-260.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Expression of Lewis-a glycans on polymorphonuclear leukocytes augments function by increasing transmigration
    Authors: JC Brazil, R Sumagin, SR Stowell, G Lee, NA Louis, RD Cummings, CA Parkos
    J. Leukoc. Biol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human MBL Protein, CF
By Anonymous on 12/19/2018
Application: Immunoassay Standard