Recombinant Human MFG-E8 Protein, CF

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2767-MF-050
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Recombinant Human MFG-E8 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 5 x 104 cells/well are added to Recombinant Human MFG‑E8 coated plates (10 µg/mL, 100 µL/well), approximately 45‑75% will adhere after 60 minutes at 37 °C.
Source
Mouse myeloma cell line, NS0-derived human MFG-E8 protein
Leu24-Cys387, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu24
Predicted Molecular Mass
41.6 kDa
SDS-PAGE
40-58 kDa, reducing conditions

Product Datasheets

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2767-MF

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2767-MF

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: MFG-E8

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66-75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Human MGF-E8 contains one N-terminal EGF-like domain and two C‑terminal F5/8-type discoidin-like domains (2). It shares 63% and 61% aa sequence identity with comparable regions of mouse and rat MFG-E8, respectively. Shorter isoforms of human MFG-E8 may have N-terminal deletions (beginning near the end of the first discoidin-like domain), internal deletions (lacking either the EGF-like domain or the central region of the second discoidin-like domain), or C‑terminal deletions (truncated within the second discoidin-like domain) (3). A 50 aa internal proteolytic fragment of human MFG-E8 (known as Medin) is a major component of aortic medial amyloid deposits (4). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (5, 6). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (7). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (8, 9) and of apoptotic B cells during germinal center reactions (10, 11). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (12, 13). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (14). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (15).

References
  1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
  2. Couto, J.R. et al. (1996) DNA Cell Biol. 15:281.
  3. Yamaguchi, H. et al. (2010) Eur. J. Immunol. 40:1778.
  4. Haggqvist, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:8669.
  5. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
  6. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
  7. Hanayama, R. et al. (2002) Nature 417:182.
  8. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
  9. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
  10. Hanayama, R. et al. (2004) Science 304:1147.
  11. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
  12. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
  13. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
  14. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
  15. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.
Long Name
Milk Fat Globule EGF Factor 8
Entrez Gene IDs
4240 (Human); 17304 (Mouse)
Alternate Names
BA46; Breast epithelial antigen BA46; EDIL1; hP47; HsT19888; lactadherin; Lactahedrin; Medin; MFG1; MFGE8; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; Milk fat globule-EGF factor 8; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47

Citations for Recombinant Human MFG-E8 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Unravelling heterogeneous effects of cancer?associated fibroblasts on poor prognosis markers in breast cancer EM?G3 cell line: In vitro?targeted treatment (anti?IL-6, anti?VEGF-A, anti?MFGE8) based on transcriptomic profiling
    Authors: Urban, L;Novák, Š;?oma, M;Dvo?ánková, B;Lacina, L;Šáchová, J;Hradilová, M;Svato?ová, P;Kolá?, M;Strnad, H;B?ezinová, J;Smetana, K;Gál, P;Szabo, P;
    Oncology reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. EDIL3 as an angiogenic target of immune exclusion following checkpoint blockade
    Authors: Tabasum, S;Thapa, D;Giobbie-Hurder, A;Weirather, JL;Campisi, M;Schol, PJ;Li, X;Li, J;Yoon, CH;Manos, MP;Barbie, DA;Hodi, FS;
    Cancer immunology research
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  3. Development of human cell-expressed tag-free rhMFG-E8 as a radiation mitigator and a therapeutic for acute kidney injury
    Authors: Chaung, W;Zhang, F;Ma, G;Yen, H;Jacob, A;Brenner, M;Wang, P;
    Research square
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. MFG-E8 stabilized by deubiquitinase USP14 suppresses cigarette smoke-induced ferroptosis in bronchial epithelial cells
    Authors: Y Cui, L Luo, Z Zeng, X Liu, T Li, X He, Y Ma, W Meng, H Zeng, Y Long, D Zong, Y Chen
    Cell Death & Disease, 2023-01-03;14(1):2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. MFG-E8 (LACTADHERIN): a novel marker associated with cerebral amyloid angiopathy
    Authors: P Marazuela, M Solé, A Bonaterra-, J Pizarro, J Camacho, E Martínez-S, HB Kuiperij, MM Verbeek, AM de Kort, FHBM Schreuder, CJM Klijn, L Castillo-R, O Pancorbo, D Rodríguez-, F Pujadas, P Delgado, M Hernández-
    Acta neuropathologica communications, 2021-09-16;9(1):154.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Identification and Validation of Nutrient State-Dependent Serum Protein Mediators of Human CD4+ T Cell Responsiveness
    Authors: K Han, K Singh, MJ Rodman, S Hassanzade, Y Baumer, RD Huffstutle, J Chen, J Candia, F Cheung, KER Stagliano, M Pirooznia, TM Powell-Wil, MN Sack
    Nutrients, 2021-04-28;13(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Extracellular vesicles produced by bone marrow mesenchymal stem cells attenuate renal fibrosis, in part by inhibiting the RhoA/ROCK pathway, in a UUO rat model
    Authors: Z Shi, Q Wang, Y Zhang, D Jiang
    Stem Cell Res Ther, 2020-06-26;11(1):253.
    Species: Human, Rat
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  8. KCNJ13 Gene Deletion Impairs Cell Alignment and Phagocytosis in Retinal Pigment Epithelium Derived from Human-Induced Pluripotent Stem Cells
    Authors: Y Kanzaki, H Fujita, K Sato, M Hosokawa, H Matsumae, F Shiraga, Y Morizane, H Ohuchi
    Invest. Ophthalmol. Vis. Sci., 2020-05-11;61(5):38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. MFG-E8 mediates arterial aging by promoting the proinflammatory phenotype of vascular smooth muscle cells
    Authors: HY Chiang, PH Chu, TH Lee
    J. Biomed. Sci., 2019-08-30;26(1):61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Bacillus anthracis Edema Toxin Inhibits Efferocytosis in Human Macrophages and Alters Efferocytic Receptor Signaling
    Authors: Z Pan, EK Dumas, C Lawrence, L Pate, S Longobardi, X Wang, JA James, S Kovats, AD Farris
    Int J Mol Sci, 2019-03-07;20(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Recombinant milk fat globule-EGF factor-8 reduces apoptosis via integrin ?3/FAK/PI3K/AKT signaling pathway in rats after traumatic brain injury
    Authors: YY Gao, ZH Zhang, Z Zhuang, Y Lu, LY Wu, ZN Ye, XS Zhang, CL Chen, W Li, CH Hang
    Cell Death Dis, 2018-08-28;9(9):845.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  12. Method for measuring extracellular flux from intact polarized epithelial monolayers
    Authors: MA Calton, MO Beaulieu, G Benchorin, D Vollrath
    Mol. Vis., 2018-06-25;24(0):425-433.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Atorvastatin Inhibits the HIF1?-PPAR Axis, Which Is Essential for Maintaining the Function of Human Induced Pluripotent Stem Cells
    Authors: Y Nakashima, C Miyagi-Shi, H Noguchi, T Omasa
    Mol. Ther., 2018-06-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells
    Authors: G Inana, C Murat, W An, X Yao, IR Harris, J Cao
    J Transl Med, 2018-03-13;16(1):63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Quantified F-Actin Morphology Is Predictive of Phagocytic Capacity of Stem Cell-Derived Retinal Pigment Epithelium
    Authors: C Müller, C Charniga, S Temple, SC Finnemann
    Stem Cell Reports, 2018-02-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. MFG-E8 overexpression promotes colorectal cancer progression via AKT/MMPs signalling
    Authors: Q Zhao, L Xu, X Sun, K Zhang, H Shen, Y Tian, F Sun, Y Li
    Tumour Biol., 2017-06-01;39(6):1010428317707.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. A novel affinity-based method for the isolation of highly purified extracellular vesicles
    Sci Rep, 2016-09-23;6(0):33935.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  18. Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8.
    Authors: Abe T, Shin J, Hosur K, Udey M, Chavakis T, Hajishengallis G
    J Immunol, 2014-06-23;193(3):1383-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. ERK5 activation in macrophages promotes efferocytosis and inhibits atherosclerosis.
    Authors: Heo K, Cushman H, Akaike M, Woo C, Wang X, Qiu X, Fujiwara K, Abe J
    Circulation, 2014-04-28;130(2):180-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Neutrophil microparticles modulate cytokine production by natural killer cells.
    Authors: Pliyev B, Kalintseva M, Abdulaeva S, Yarygin K, Savchenko V
    Cytokine, 2013-12-16;65(2):126-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. The interaction between CD300a and phosphatidylserine inhibits tumor cell killing by NK cells.
    Authors: Lankry D, Rovis T, Jonjic S, Mandelboim O
    Eur J Immunol, 2013-06-21;43(8):2151-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  22. Comparative proteomic analysis of rat aorta in a subtotal nephrectomy model.
    Authors: Lin YP, Hsu ME, Chiou YY
    Proteomics, 2010-07-01;10(13):2429-43.
    Applications: ELISA (Standard)
  23. Transglutaminase 2 is needed for the formation of an efficient phagocyte portal in macrophages engulfing apoptotic cells.
    Authors: Toth B, Garabuczi E, Sarang Z, Vereb G, Vamosi G, Aeschlimann D, Blasko B, Becsi B, Erdodi F, Lacy-Hulbert A, Zhang A, Falasca L, Birge RB, Balajthy Z, Melino G, Fésüs L, Szondy Z
    J. Immunol., 2009-02-15;182(4):2084-92.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance

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Recombinant Human MFG-E8 Protein, CF
By Jason Miller on 01/13/2016
Application: In vitro bioactivity in cell culture
Reason for Rating: Bioactivity is reproducible, and endotoxin levels are consistently low as promised.

We use MFG-E8 as a ligand for phagocytosis assays involving AVB5 integrin receptor. Our reading is whether or not we see lipofuscin buildup from phagocytic overload. With MFG-E8 at 1.5ug/mL, we see significantly more lipofuscin buildup than without the recombinant protein, as applied to primary cell culture.