Recombinant Human MFRP Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Supplemental Products

Recombinant Human MFRP Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit the activity of Recombinant Human BMP‑1/PCP (Catalog # 1927-ZN) on the cleavage of a fluorogenic peptide substrate Mca-YVADAPK(Dnp)-OH (Catalog # ES007).

Recombinant human MFRP at 30 µg/mL will reduce rhBMP-1 activity by 30‑60% when measured with 1 µg of BMP-1 and 10 µM ES007 in 100 µL of 10 mM HEPES, 0.01% Brij-35, pH 7.5 at room temperature.

Mouse myeloma cell line, NS0-derived human MFRP protein
Ser101-Pro579, with an N-Terminal 10-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
52.7 kDa
90-105 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: MFRP

MFRP (membrane-type frizzled-related protein) is a 65 kDa, type II transmembrane protein related to both Tolloid proteases and frizzled-domain containing Wnt pathway proteins (1 - 4). Human MFRP is 579 amino acids (aa) in length (3, 5, 6). It contains a 69 aa cytoplasmic region, a 21 aa transmembrane segment, and a 489 aa extracellular domain (ECD). The ECD is characterized by the presence of two LDLR class A repeats, two CUB domains, and a C-terminal cysteine-rich/frizzled domain. The mRNA for MFRP is highly unusual in that it is dicistronic; that is, it contains two independent ORFs, one for MFRP and one for a functionally-related protein termed CTRP5/C1qTNF5 (4, 7). CTRP5 is a secreted, 25 kDa short-chain collagen that contains a C1q-type domain (6, 8). In prokaryotes, polycistronic transcripts exist that contain functionally-interactive molecules. This would also appear to be the case for MFRP and CTRP5. CTRP5 is suggested to bind to membrane MFRP via the C1q and CUB domains, respectively. This is positioned to generate a receptor-coreceptor complex that binds select Wnts such as Wnt-1 and/or Wnt-10b (4, 6, 7). MFRP has multiple documented mutations. In human, these are associated with hyperopia (severe farsightedness). The mutations result in premature truncations (3, 9). MFRP is expressed in retinal pigment epithelium, ciliary epithelium, and keratinocytes (4, 7, 10, 11). Human MFRP ECD is 70% aa identical to mouse ECD.

  1. Katoh, M. and M. Katoh (2005) Oncol. Rep. 14:1381.
  2. Jones, S.E. and C. Jomary (2002) BioEssays 24:811.
  3. Sundin, O.H. et al. (2005) Proc. Natl. Acad. Sci. USA 102:9553.
  4. Mandal, M.N.A. et al. (2006) Invest. Ophthalmol. Vis. Sci. 47:5514.
  5. Katoh, M. (2001) Biochem. Biophys. Res. Commun. 282:116.
  6. Shu, X. et al. (2006) Hum. Mol. Genet. 15:1680.
  7. Kameya, S. et al. (2002) Hum. Mol. Genet. 11:1879.
  8. Hayward, C. et al. (2003) Hum. Mol. Genet. 12:2657.
  9. Ayala-Ramirez, R. et al. (2006) Mol. Vis. 12:1483.
  10. Yang, L. et al. (2006) J. Dermatol. Sci. 42:111.
  11. Ayyagari, R. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46:3363.
Long Name
Membrane Frizzled-related Protein
Entrez Gene IDs
83552 (Human); 259172 (Mouse)
Alternate Names
MCOP5; membrane frizzled-related protein; Membrane-type frizzled-related protein; MFRP; MGC32938; NNO2; rd6


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Fluorogenic Peptide Substrates

Recombinant Enzymes

Reconstitution Buffers

Reviews for Recombinant Human MFRP Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human MFRP Protein, CF and earn rewards!

Have you used Recombinant Human MFRP Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review