Recombinant Human MuSK Avi-tag His-tag Biotin Protein, CF

Catalog # Availability Size / Price Qty
Biotinylated Recombinant Human MuSK Avi-tag His-tag Protein SDS-PAGE.
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Recombinant Human MuSK Avi-tag His-tag Biotin Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When 2.00 μg/mL of Biotinylated Recombinant Human MuSK Avi-tag His-tag protein is immobilized onto Streptavidin coated plate (Catalog # CP004), in the presence of Recombinant Human Agrin (Catalog # 6624-AG), it binds to Recombinant Human LRP-4 (Catalog # 5948-LR) with an ED50 of 0.500-3.00 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human MuSK protein
Accession # O15146.1
Accession #
N-terminal Sequence
Structure / Form
Biotinylated via Avi-tag.
Predicted Molecular Mass
55 kDa
59-67 kDa, under reducing conditions.

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human MuSK Avi-tag His-tag Protein (Catalog # AVI10189) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 59-67 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: MuSK

Musk (muscle-specific kinase) is a 53 kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family (1). It contains a 472 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 353 cytoplasmic domain (1). Within the ECD, Human Musk shares 91% and 92% aa sequence identity with mouse Musk and rat Musk, respectively. It is found in the postsynaptic membrane of skeletal muscle motor endplates (2). Human Musk has multiple isoforms. One contains deletions at residues 307‑394 and 454‑461, while a second is a short soluble form that contains residues 120‑209 plus a unique 24 aa C‑terminal tail (3). Musk binds the heparin sulfate proteoglycan Agrin to promote acetylcholine receptor clustering. It has also been found to bind with low-density lipoprotein receptor-related protein 4 (LRP4) (4), Wnt ligands (5), Biglycan (6), and ColQ (7). Recent studies have showed that Musk does not bind Agrin directly, but enhanced the MuSK-LRP4 interaction (7, 8). When Agrin binds to the N-Terminal region of LRP4, this promotes the association of LRP4 and Musk, which then stimulates Musk kinase activity (9, 10). Our Avi-tag Biotinylated human Musk features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

  1. Valenzuela, D.M. et al. (1995) Neuron. 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
  4. Kim, N. et al. (2008) Cell 135:334.
  5. Banerjee, S. et al. (2011) Development 138:3287.
  6. Amenta, A.R. et al. (2012) J Neurosci 32:2324.
  7. Otsuka, K. et al (2015) Scientific Reports. 5:13928.
  8. Zhang, B. et al. (2008) Neuron 60:285.
  9. Zhang, W. et al. (2011) J Biol Chem. 286(47):40624.
  10. Burden, S.J. et al. (2017) CSH Perspectives Biol. 5:a009167.
Long Name
Muscle-specific Receptor Tyrosine Kinase
Entrez Gene IDs
4593 (Human); 18198 (Mouse); 81725 (Rat); 102127677 (Cynomolgus Monkey)
Alternate Names
EC 2.7.10; EC; MGC126323; MGC126324; muscle, skeletal, receptor tyrosine kinase; MuSK; skeletal receptor tyrosine-protein kinase


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