Recombinant Human Netrin-G1a His-tag Protein, CF

Catalog # Availability Size / Price Qty
10019-NG-050
Recombinant Human Netrin-G1a His-tag Protein, CF Binding Activity
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Product Details
Citations (1)
FAQs
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Recombinant Human Netrin-G1a His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human NGL-1/LRRC4C (Catalog # 4899-NR) is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Netrin-G1a that produces 50% optimal binding response is 0.2-1 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Netrin-G1a protein
His29-Gly503, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Val19 & His29
Structure / Form
Monomer
Predicted Molecular Mass
55 kDa
SDS-PAGE
69-76 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10019-NG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human NGL-1/LRRC4C (Catalog # 4899-NR) is coated at 2 μg/mL, 100 μL/well, Recombinant Human Netrin-G1a (Catalog # 10019-NG) binds with an ED50 of 0.2‑1 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Netrin‑G1a was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 69-76 kDa and 55- 66 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Netrin-G1a

NTNG-1, known as Netrin-G1 or alternatively Laminet-1 (Gene name: NTNG1), is a member of the UNC-6/Netrin family of proteins. Human Netrin-G1 is a 55 kDa protein and shares 100% homology with its crab-eating macaque, 98% homology with its rat homolog, and 97% homology with its mouse homolog. Netrin-G1 is synthesized as a 539 amino acid (aa) precursor with a 28 aa signal sequence, a 251 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, a GPI (glycosylphosphatidylinisotol) anchor (Ser510), and a C-terminal 28 aa propeptide that is removed in mature Netrin-G1 (1, 3, 4). Netrin-G1 interacts with its ligand, Netrin-G ligand 1 (NGL1) to regulate neuron growth and patterning, axonal subdendritic differentiation, and synapse formation throughout development (1, 2, 4, 6). Abnormal expression of Netrin-G1 via SNPs (single nucleotide polymorphisms) have been implicated in pathogenesis for schizophrenia (5). Netrin-G1 has widespread expression only in vertebrates, occurring in olfactory mitral cells, cells of the inferior colliculus (hearing), dorsal thalamus (behavior), and cells of the deep cerebellar nuclei and inferior olive (motion) (3).

References
  1. Seiradake, E. et al. (2011) EMBO J. 30:4479.
  2. Song, Y. et al. (2013) J Cell Sci. 126:4926.
  3. Nakashiba, T. et al. (2000) J Neuroscience. 20:6540.
  4. Matsukawa, H. et al. (2014) J Neuroscience. 34:15779.
  5. Zhu, Y. et al. (2011) J Genet. 90:499.
  6. Nishimura-Akiyoshi, S. et al. (2007) Neuroscience. 104:14801.
Entrez Gene IDs
22854 (Human); 80883 (Mouse)
Alternate Names
NetrinG1a; Netrin-G1a; NTG1; NTNG1

Citation for Recombinant Human Netrin-G1a His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Isolation and characterization of myogenic precursor cells from human cremaster muscle
    Authors: N Naldaiz-Ga, M Goicoechea, IM Aragón, V Pérez-Lópe, S Fuertes-Al, B Herrera-Im, A López de M, R de Luna-Di, PM Baptista, MA Fernández, MF Lara, A Izeta
    Sci Rep, 2019;9(1):3454.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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